...Addgene is very excited to share the very first edition of our Antibodies 101 eBook! The latest addition... always, don’t hesitate to reach out to help@addgene.org with any thoughts, questions, or feedback. Happy...

... hear from Addgene’s former blog editor Tyler Ford on how initiative landed him at Addgene. What is initiative... Once in-person, peer-mentoring groups at Addgene have gone virtual!   2. Practice public speaking...almost every career talk I present, I’m asked what Addgene “looks for” when hiring a scientist. Of course,... comments below! Additional resources on the Addgene blog Read our science career blog posts Check ...

...Find plasmids from this paper at Addgene. Additional Resources the Addgene Blog Learn More about SunTag... remained elusive. Two techniques developed by Addgene depositors have made it easier to track translation...PMC2746483 Find ribosome profiling plasmids at Addgene. 2. Halstead, James M., et al. “Translation. An...: PMC4451088 Find plasmids from this paper at Addgene. 3. Yan, Xiaowei, et al. “Dynamics of Translation... Brightest Fluorescent Protein Resources on Addgene.org Find Fluorescent Protein Plasmids Check Out ...

...number to the capsid. Most vectors provided by Addgene are pseudotypes using the genomic structure of ...repository and can be selected as a serotype for Addgene’s Packaged on Request service. Some AAV1 tools are...repository and can be selected as a serotype for Addgene’s Packaged on Request service. Some AAV2 tools are...repository and can be selected as a serotype for Addgene’s Packaged on Request service. Some AAV5 tools are...service and can be selected as a serotype for Addgene’s Packaged on Request service. Some AAV8 tools are...2023). AAV9 packaging plasmids are available at Addgene. AAV9 vectors are not available through our Packaged...repository and can be selected as a serotype for Addgene’s Packaged on Request service.  Some AAVrg tools...

...posts @Addgene: The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years Addgene’s Top...2011, it was only recently made available through Addgene and we want to highlight it for use in your research...systems have been developed and deposited with Addgene which allow scientists to use CRISPR-Cas technology...determining the structures are now available from Addgene - bfloGFPa1 and bfloGFPc1. Bomati et al., Sci ...further reading about SpyLigase technology, read the Addgene interview with Mark Howarth. Shining a light...ChrimsonR, CsChR, and CoChR have been deposited to Addgene, including lentiviral and AAV expression vectors... of various optogenetics plasmid tools, visit Addgene’s optogenetics guide. More optogenetics tools...

...addition to useful plasmids and toolkits available at Addgene.  C. elegans open resources The C. elegans Neuronal...Kyoda et al., 2012).  C. elegans plasmid tools at Addgene In addition to the wealth of open resources described...highlight a few toolkits that we have available at Addgene that were discussed primarily at the workshops ...interest.  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene, we have the entire SapTrap toolkit from the Jorgensen...resources about C. elegans and the plasmids we have at Addgene check out our worm expression resources page. This...PMCID: PMC4689222. Additional resources on the Addgene blog Learn more about SapTrap Read about CRISPR...Browse our Plasmids 101 blog posts Resources on Addgene.org Visit our worm plasmids and resources page Browse...

...maps. Resources on Addgene.org Sequence Analysis of your Addgene Plasmid Addgene Plasmid Guide     ...This post was updated on Dec 4, 2017. At Addgene, we continually use the Basic Local Alignment Search...databases, and elements of the BLAST search pages. At Addgene, we use blastn to identify any discrepancies in... viewing blastx results. When using blastx at Addgene, we use the default “Non-redundant protein sequences...NCBI BLAST website. Additional Resources on the Addgene Blog: Plasmids 101: How to Verify Your Plasmid...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...discuss the importance of actionability on the Addgene blog and how SITN short-form blog posts help readers...Additional Resources on the Addgene Blog Resume Writing for Non-academic Science Careers 10 Steps to a Perfect...

...was contributed by guest blogger, member of the Addgene Advisory Board, and Associate Director of the Genetic... that model. Both libraries are available from Addgene as pools provided in two sets, each with 4 gRNAs...individual plasmid formats A first for a library at Addgene, you can also order the individual lentiGuide gRNA...PubMed Central PMCID: PMC4744125. Resources on the Addgene Blog Learn to Conduct Genome-wide Screens with...CRISPR for Single Base Editing Resources on Addgene.org Browse Our New and Improved Pooled Library Page...

...Additional resources on the Addgene blog Read about company culture at Addgene See some of our previous ...costumes, and spooky science stories. Yes, please! At Addgene, we love finding ways to incorporate science themes...teams dressed up as plasmids, Blugene, and the Addgene ping pong table. This year, in addition to the ...Halloween is no exception. For the past 10+ years, Addgenies have formed teams, chosen a theme for the costume...

...to highlight some of the top articles from the Addgene blog this year. Spoiler alert: many of the top ...SARS-CoV-2, including many with plasmids deposited at Addgene! 2. DNA Purification Without a Kit by Beth Kenkel...

...community, so email us at blog@addgene.org if you are interested. Addgene's Most Popular Posts of 2014 ...year in science – as seen through the lens of Addgene’s blog and plasmid repository. Our blog is just ...

...the wide array of Cas9s (many available through Addgene’s repository) and make it easy to find your perfect...variety of species and cell types can be found on Addgene’s website. Activating target genes using dCas9-based...variety of species and cell types can be found on Addgene’s website. Fig. 3: Cas9 repressors include...What do i do next? Designing or selecting a sgRNA Addgene may carry a validated sgRNA for your target gene...Next CRISPR Experiment Cas9 expression systems Addgene carries a variety of Cas9 containing plasmids that... ). 368, 290–296 (2020). More resources on the Addgene blog Read all the posts in our CRISPR 101 Series...Library Experiments Additional resources on the Addgene website Find CRISPR Plasmids Catch up on your ...

...promoter in rat. Contribute your AAV data Just like Addgene’s plasmid repository, the AAV Data Hub is a community-driven...only accepting data for materials distributed by Addgene.  Future directions As the AAV Data Hub grows, ...accelerate your research. Sincerely, Melina & The Addgene Team ...

...plasmids you’d like to deposit with Addgene, be sure to visit addgene.org/deposit. Who knows, maybe yours ... articles cite the use of pMD2.G obtained from Addgene. Skimming the list, one gets a sense of the broad...searchable list here). Tips for using pMD2.G At Addgene, we use pMD2.G for producing our ready-to-use lentivirus...Lentiviral Transduction Additional Resources on Addgene.org Check out our Viral Service Learn more about...

...plasmids with Addgene. Additional control plasmids for the TKO library will be available at Addgene soon. Hart...cellular compartments (cytosol, Addgene catalog #71245; mitochondria, Addgene catalog #71247) Rosella provides...read? Listen to our hot plasmids segment on the Addgene Podcast. View Plasmids from:  March (Q1) | June...sequencing. The STARR-seq backbone is available from Addgene in both human and fly versions. Arnold, et al....alongside a growing number of CRISPR libraries from Addgene’s repository that can be used to perform screens...PMID: 24395366 Oxford Drosophila CRISPR library Addgene has had the privilege of distributing several human...promoter and Cas9 from the actin 5C promoter. Addgene’s fly library web page offers more details and protocols...

...tools. Find the mouse CRISPR knockout library at Addgene. Henriksson et al., Cell, 2019. PubMed PMID: 30639098...cardiovascular disease. Find the LipoGlo plasmids at Addgene! Lawler et al, QJM: An International Journal of...systems. Find these CRISPR activation plasmids at Addgene. Liu et al., Nature Communications, 2019. PubMed...worms and have made these constructs available at Addgene. These include Arch(D95N) and QuasAr, as well as...biosensors at Addgene. Hashemi et al., PNAS. 2019, PubMed PMID 31371514. ... Various Addgenies...

...Additional Resources on the Addgene Blog Read Additional Cancer Blog Posts Addgene's Cancer Collection Pages... between tumor and immune cells in real time. Addgene depositor Professor Max Krummel from UCSF showed...engineering (Ref 5)  as described by Blue Flame Addgene depositor Professor Michel Sadelain from the Memorial...poster is pTomo-Ras which has been deposited at Addgene was used to produce the glioblastoma model for ...with the scientific community, contact deposit@addgene.org to find out more about our free deposit and distribution...: Deciphering the Relationship Resources on Addgene.org Browse Cancer Plasmids Browse Immunology Plasmids...

... planning an experiment to performing one.  At Addgene, over 120 labs have deposited CRISPR reagents, ...already been shown to work in CRISPR experiments. Addgene’s dedication to resource sharing has led us to develop...table columns are target gene, species, sequence, Addgene plasmid ID (if we have one associated with the ...Sharing. 2016. In press. Additional Resources on the Addgene Blog Learn How to Design Your gRNA Use the CRISPR...This post was written jointly by Addgenies Brook Pyhtila and Nicole Waxmonsky Resource sharing shortens...of Our CRISPR Posts Additional Resources on Addgene.org Browse gRNA Design Tools Read Our CRISPR Guide...

...adeno-associated viral (AAV) vectors available at Addgene are suitable for in vivo studies, our scientists...measuring viral titer and assessing purity. But Addgene is also conducting more rigorous quality control...plexWell technology, we can now perform NGS at Addgene to sequence all the DNA packaged inside the viral...AAV-68544, the best match for the contaminant is Addgene plasmid #44362. Method validation, limitations,...commonly performed for research-grade materials. Addgene is going the extra mile and pioneering the systematic... Press, pp 247–278 Additional resources on the Addgene blog Check out our Viral Vectors Featured Topic...discuss. We are also available by phone or at help@addgene.org to answer your questions about our quality control...