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  1. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...transcriptase (RT), which induces two double-strand breaks and deletes the intervening sequence. As with other...

  2. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...AAV8 vectors with broadly active promoters from subretinal injections of neonatal mouse eyes at two different...

  5. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ... tags influence behavior. A conference and a breakthrough! During my graduate studies I attended a scientific...

  7. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...broke down RNases before RNases had a chance to break down RNA upon cell lysis.  In the late 1970’s, Chirgwin...

  10. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...proteins to form a tetramer. A double-strand DNA break occurs within the core spacer of each loxP site ...

  13. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...doubt that CRISPR/Cas9 technology has been a breakthrough for the genome-editing field and the greater...

  14. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...Search-and-replace genome editing without double-strand breaks or donor DNA. Nature, 576(7785), 149–157. https...

  16. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ... the Fragmid system, the Fragmid website, which breaks the design process down into four easy steps, provides...
Showing: 221 - 240 of 316 results