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  1. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...of saying “I have excellent communication skills” try giving an example, like “I have extensive communication... Should I write a cover letter? If you’re in a country where cover letters are common, the answer is usually...skills-based ones, may not use them very often. Similarly, entry-level jobs are less likely to require cover letters...if ever, sees cover letters submitted for their entry-level jobs and does not require or request them ...format regardless of whether you’re in academia, industry, or nonprofits. Like any other letter, they have...positions. Many did not require cover letters for entry-level positions, as at that stage, cover letters...
  2. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ... these parameters, here are a few things you can try: Increase the number of cycles. After additional...
  3. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...opportunity for your classroom, I highly recommend you try implementing one. It's likely only a matter of time... in their careers? A CURE for Biochemistry Undergraduate biochemistry courses are often associated with...Minnesota State (and now Professor of Chemistry and Biochemistry at the University of San Diego), decided...Sharpless was awarded this year’s Nobel Prize in Chemistry, he joined an elite club, becoming just the fifth...presented their findings at the American Society for Biochemistry and Molecular Biology Annual Meeting. Some of...inquiry and research-based learning in a year-long biochemistry laboratory: Part I – guided inquiry – purification...dehydrogenase, and green fluorescent protein. Biochemistry and Molecular Biology Education. 38(5):317-323...
  4. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...the scales in your favor for an HDR edit, you can try one or more of the below suggestions. Disable the...
  5. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...it was promising enough to keep exploring and to try developing a variant with an enhanced response. Evolution...researchers from a range of fields, like quantum chemistry and protein biophysics, as well as inventors and...effects relevant to avian magnetoreception. Nature Chemistry, 8(4), 384–391. https://doi.org/10.1038/nchem.2447...
  6. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...their protein of interest. After several decades of trying to address these challenges, researchers have amassed... used for western blots, IP, co-IP, IF, flow -cytometry; can occassionally interfere with protein folding... used for western blots, IP, co-IP, IF, flow -cytometry, but rarely used for purification as elution requires...
  7. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...not always a straightforward process. Rather than try to directly detect your gene of interest, scientists...advantage of an element called an Internal Ribosome Entry Site (IRES) to allow for initiation of translation...encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. Jang et...
  8. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...exhaustive, but should be a good place to start when trying to pick your perfect promoter! Eukaryotic Promoters...E. coli tryptophan operon Repressible Gets turned off with high levels of cellular tryptophan. lac General...
  9. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...Okay, let’s play. I’ll list the examples and you try to guess the category: ELISA...qPCR...Digital droplet... quantitation of DNA copy number." Analytical chemistry 83.22 (2011): 8604-8610.4. Hindson CM, Chevillet...
  10. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...hard to conceptualize where the populations are, try drawing a quadrant over the plot that divides it ...data in the literature. Data reported from flow cytometry experiments can be a little challenging to understand... on how to read a flow plot.  Dot plots Flow cytometry is a method that allows users to analyze single...Created with biorender.com.   The goal of flow cytometry is to use increasingly more stringent markers ...in the same order. However, in an actual flow cytometry run, all markers are read in parallel.  Let’s ...more things to learn about when diving into flow cytometry data. There’s different ways to use FSC and SSC...resources on the Addgene blog Conventional vs. Flow Cytometry Plasmid-based Recombinant Monoclonal Antibodies...
Showing: 201 - 220 of 450 results