...issues related to AAV vectors, many from people trying to answer the exact same question I was: What AAV...

...it was promising enough to keep exploring and to try developing a variant with an enhanced response. Evolution...researchers from a range of fields, like quantum chemistry and protein biophysics, as well as inventors and...effects relevant to avian magnetoreception. Nature Chemistry, 8(4), 384–391. https://doi.org/10.1038/nchem.2447...

...factors may impact the dynamic range of the assay. Try to minimize these.  Review previous literature or...

...of saying “I have excellent communication skills” try giving an example, like “I have extensive communication... Should I write a cover letter? If you’re in a country where cover letters are common, the answer is usually...skills-based ones, may not use them very often. Similarly, entry-level jobs are less likely to require cover letters...if ever, sees cover letters submitted for their entry-level jobs and does not require or request them ...format regardless of whether you’re in academia, industry, or nonprofits. Like any other letter, they have...positions. Many did not require cover letters for entry-level positions, as at that stage, cover letters...

...and free for all non-profit uses. We invite you to try ICE today and let us know how it works for you! Read...the sample name or on its corresponding bar graph entry. Clicking to initiate the in-depth analysis will...

... against each other at the same time in order to try and access the entire interactome of a set of proteins...

...their protein of interest. After several decades of trying to address these challenges, researchers have amassed... used for western blots, IP, co-IP, IF, flow -cytometry; can occassionally interfere with protein folding... used for western blots, IP, co-IP, IF, flow -cytometry, but rarely used for purification as elution requires...

... 3,000 times each month. I looked this up after trying out the new protocols.io beta platform for sharing...pricing options for academic labs, departments, industry, and more to fit every lab's needs. Plus there...

...not always a straightforward process. Rather than try to directly detect your gene of interest, scientists...advantage of an element called an Internal Ribosome Entry Site (IRES) to allow for initiation of translation...encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. Jang et...

...implicated in human diseases. His spare time is spent trying to raise the alpha isoform of GSK-3 to the same...

...exhaustive, but should be a good place to start when trying to pick your perfect promoter! Eukaryotic Promoters...E. coli tryptophan operon Repressible Gets turned off with high levels of cellular tryptophan. lac General...

...insertion site. If you are using the ivTRT method, try to make the first two bases of the left homology ...

...different tasks. I use google calendar for this and try to give myself flexibility by never placing too many...

...Okay, let’s play. I’ll list the examples and you try to guess the category: ELISA...qPCR...Digital droplet... quantitation of DNA copy number." Analytical chemistry 83.22 (2011): 8604-8610.4. Hindson CM, Chevillet...

...question of how we can support researchers as they try to keep up with the growing amount of interdisciplinary...

...Volunteer moderators set the rules for these forums and try to keep discussions on-topic and, at least sometimes...

... know a lot about and that you care about. Don’t try to present topics that are unfamiliar to you because...Joanne's 10 Things Scientists Ask about Finding an Industry Job Talk What’s Your Story? By Herminia Ibarra...

...directed mutagenesis. In addition, you may want to try different concentrations of template (e.g. 0.1-1.0...in the polymerase chain reaction." Analytical Biochemistry 209.2 (1993): 284-290. PubMed PMID: 8470801....

... Addgene. If you’ve given any of these systems a try, we’d love to hear your feedback!   Thank you to...Jieze Zhang is an undergraduate student studying biochemistry at the University of Southern California. He...obtained his Ph.D. from Shanghai Institute of Biochemistry, Chinese Academy of Sciences. He joined Shanghai... new generation of engineered cells for green chemistry and biomedical applications.   Yiwen Zhang entered...

...Decades later and after years of painstaking work trying to isolate the causative gene for Rett Syndrome...