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  1. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...folder: You will often use the same data files for multiple analyses. Rather than copying these files to your...to using dates as labels, since I often work on multiple projects in a given day. For example, instead ...

  2. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ... Type IIS restriction sites to easily assemble multiple DNA fragments. This system has been used to construct...controlled by Cre recombination. Each cell can have multiple copies of the transgene that recombine independently...

  3. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...methylation like XbaI, ClaI, etc. If you are doing multiple digestion be sure that the buffers and temperature...molar ratio. Assembly of the fragments There are multiple ways you can assemble the different parts of a...

  5. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ... the visible spectrum, so they are great for multiplexing and can be used in a variety of assay types....detection of low abundance targets with significant multiplexing potential. You will find antibody-oligonucleotide...

  6. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ... transcription factors with important roles in Multiple Myeloma), it is important to ensure lack of degradation...system was employed to evaluate degradation of multiple FKBP12F36V-tagged proteins using our lentiviral...

  8. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...That way, you can run one PCR, one gel, etc. for multiple plasmids at once. Over time, this can save you... Multiple Authors...

  9. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...degradation. Cas3 DNA digestion can proceed up to multiple kilobases, but only in one direction. Cas3 is ...used in lieu of siRNA or shRNA for efficient, multiplexable RNA knockdown. Cas13 has also stirred up interest...

  10. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...tiny DNA cutting ninjas that deliver CRISPR to multiple types of targets: primary cells, embryos, and ...structural proteins, such as the MLV protein Gag, multimerize and spontaneously assemble into particles at ...

  11. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ...genome, they are not commonly used to introduce multiple mutations. This is because as more mutations are...challenge that may arise when crossing mice with multiple mutations is if the mutations are on the same ...

  12. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...elegant system that allows for the assembly of multiple DNA fragments efficiently and accurately in a ...system will allow investigators to rapidly create multiple synthetic chromatin-modifying proteins quickly...

  13. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...binding of the biosensor to endogenous calmodulin. Multiplexing Are you combining your calcium reading with ...you need to free up crowded optical space for multiplexing, GECO sensors are available in green, blue, ...

  14. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...pre-designed sets, so identical searches can return multiple answers. You might want to take advantage of their...SplitSet finds optimal fusion sites to split it into multiple fragments. You can define the number of fragments...

  15. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...which time the cells are scraped, and lysed by multiple freeze-thaw cycles. The resultant lysate is your...cytopathic effect.” Amplification can be repeated multiple times (2-4 rounds) at increasing scale over the...

  16. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...editing efficiency of prime editing, whereby a multitude of configurations can be used to introduce specific...efficiencies for diverse prime editing systems in multiple cell types. Cell, 186, 1–17. https://doi.org/10.1016...

  17. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...fast-growing cell lines (such as HEK293, which multiplies every 16 hours). So if you, for example, split... its passage number. Especially in a lab where multiple people are working on different things, this will...

  18. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...According to this logic, ω-dCas9 variants recognizing multiple PAMs should have a fitness advantage over proteins... and CAA PAMs, they saw that xCas9 3.7 cleaves multiple PAMs at a higher efficiency than SpCas9: NGT (...

  19. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ..., the widespread success of CRISPR-Cas9 across multiple model organisms (3) lies in the versatility of...which could be less desirable if one has to target multiple sites in a single experiment. Also, the NGG PAM...
Showing: 201 - 220 of 455 results