We narrowed to 458 results for: invs
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TypeBlog PostPublishedJuly 16, 2020, 1:15 p.m.... placing orders per day across regions. Our investigation of model fit revealed some potential instabilities...University; and Howard Hughes Medical Institute Investigator. Liu’s research integrates chemistry and evolution...
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Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog PostPublishedFeb. 9, 2023, 2:15 p.m....ownership of their work and become more actively involved in the learning process. As trainees become more... and effort and/or perceived professional risk involved in setting up a CURE, especially if it’s a new... -
Droplet Digital PCR for AAV Quantitation
TypeBlog PostPublishedJan. 16, 2020, 2:18 p.m.... using droplet digital PCR Droplet digital PCR involves partitioning a PCR reaction mixture into approximately... Witte, Anna Kristina, et al. "A systematic investigation of parameters influencing droplet rain in the... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog PostPublishedApril 23, 2014, 1:08 p.m.... in using the 3rd-generation system is that it involves transfecting four different plasmids into the ...quickly check that a construct is functional before investing time and resources into making a stable cell line... -
Lab to Office Culture Shock
TypeBlog PostPublishedFeb. 5, 2019, 1:04 p.m....culture shock? Working in an academic research lab involves a few key features that can be difficult to replicate...sometimes wish for a stretch of time that doesn’t involve critical thinking. Former Addgenie Jessica Welch... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog PostPublishedSept. 13, 2018, 1:55 p.m....high-throughput systematic approach for identifying genes involved in a biological process. These screens provide...with ricin regulation, including almost all genes involved in the production of a cell surface glycan that... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog PostPublishedMay 8, 2018, 1 p.m....researcher see which of the multiplexed sgRNA was involved in a particular edit, and which type of edit was...open source and free for all non-profit uses. We invite you to try ICE today and let us know how it works... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog PostPublishedSept. 21, 2017, 2:07 p.m....gene commonly associated with CRISPR arrays and involved in spacer acquisition following infection, as ... we appreciate the opportunity to share these invaluable reagents with the scientific community. Note... -
How to Write a Scientific Review Article
TypeBlog PostPublishedFeb. 16, 2017, 3:30 p.m.... Perhaps you, a colleague, or your adviser was invited to write on a particular topic. Alternatively, ...review articles Whether submitting a review by invitation or by your own accord, once you have these rules... -
Plasmids 101: Gateway Cloning
TypeBlog PostPublishedJan. 12, 2017, 3:30 p.m....resources, time, and experimental needs. Since its invention in the late 1990s, Gateway cloning technology ...technology The Gateway cloning method, developed by Invitrogen, is an in vitro version of the integration and... -
Plasmids 101: Shuttle Vectors
TypeBlog PostPublishedMay 20, 2025, 1:15 p.m....vectors? The production of viral vectors typically involves shuttle vectors, also sometimes called transfer...target cells. However, while shuttle vectors are involved in the production of viral vectors, we consider... -
Antibodies 101: The Four ELISAs and When to Use Them
TypeBlog PostPublishedJuly 25, 2024, 1:15 p.m....in a competitive ELISA the reporter signal is inversely proportional to the level of antigen in the sample...in a competitive ELISA the signal intensity is inversely proportional to the amount of antigen in the sample... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog PostPublishedNov. 10, 2016, 3:30 p.m....resistance testing is requested clinically, this involves growing a standardized amount of the bacteria ...focus on creating new antibiotic therapies, and investigate more effective drug targets (ones that are less... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog PostPublishedOct. 1, 2015, 2:30 p.m....in which your circuit of interest is putatively involved in regulating the behavioral output. For example...suggests that other regions of the brain may be involved in retrieval of remote contextual memories. This... -
Adeno-associated Viruses (AAVs) for Genome Editing
TypeBlog PostPublishedMarch 27, 2018, 1:32 p.m....delivered through transfection of naked DNA. Other investigators who had been working to develop high efficiency..., which is particularly well suited for strand invasion, a key step in the homologous recombination pathway... -
Five Popular Model Organisms, Part 2
TypeBlog PostPublishedSept. 29, 2020, 1:15 p.m....making them more useful for certain studies that involve surgery or imaging. Rattus norvegicus. Image...entire developmental cycle. A majority of studies involving axolotls focus on embryonics and biochemistry.... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog PostPublishedSept. 5, 2017, 1:58 p.m....DNA repair pathway. Accomplishing this usually involves simultaneously introducing a homologous DNA repair... (Paquet, Kwart, et al 2016). For those of you involved in the tedious business of iPSC clone picking,... -
March for Science
TypeBlog PostPublishedApril 21, 2017, 2:30 p.m....march is meant to inspire scientists to be more involved in their communities (something already encouraged...mathematical constant “pi” supports scientists getting involved in politics. Disclaimer: The views presented ... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog PostPublishedNov. 16, 2021, 2:15 p.m....sample type. Sample Preparation This step can involve multiple smaller steps that all help to ensure ... Similar to Sample Preparation, this step can involve multiple smaller steps, but the aim is to preserve... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog PostPublishedJune 17, 2025, 1:15 p.m....silencing. RNA interference RNAi is a useful tool to investigate the roles of specific genes or to knock down ...term for a number of different applications that involve different types of small RNA molecules, such as...