... Perhaps you, a colleague, or your adviser was invited to write on a particular topic. Alternatively, ...review articles Whether submitting a review by invitation or by your own accord,  once you have these rules...

...resources, time, and experimental needs. Since its invention in the late 1990s, Gateway cloning technology ...technology The Gateway cloning method, developed by Invitrogen, is an in vitro version of the integration and...

...culture shock? Working in an academic research lab involves a few key features that can be difficult to replicate...sometimes wish for a stretch of time that doesn’t involve critical thinking. Former Addgenie Jessica Welch...

...high-throughput systematic approach for identifying genes involved in a biological process. These screens provide...with ricin regulation, including almost all genes involved in the production of a cell surface glycan that...

...researcher see which of the multiplexed sgRNA was involved in a particular edit, and which type of edit was...open source and free for all non-profit uses. We invite you to try ICE today and let us know how it works...

...in which your circuit of interest is putatively involved in regulating the behavioral output. For example...suggests that other regions of the brain may be involved in retrieval of remote contextual memories. This...

...sample type. Sample Preparation This step can involve multiple smaller steps that all help to ensure ... Similar to Sample Preparation, this step can involve multiple smaller steps, but the aim is to preserve...

...making them more useful for certain studies that involve surgery or imaging.  Rattus norvegicus. Image...entire developmental cycle. A majority of studies involving axolotls focus on embryonics and biochemistry....

...DNA repair pathway. Accomplishing this usually involves simultaneously introducing a homologous DNA repair... (Paquet, Kwart, et al 2016). For those of you involved in the tedious business of iPSC clone picking,...

... a suicide switch in cyanobacteria, synthetic inverters in E. coli, and biosensors in E. coli: Čelešnik...017129. Hsia, Justin, et al. "Modular Synthetic Inverters from Zinc Finger Proteins and Small RNAs." PloS...

...real-time PCR Southern blotting, named for its inventor Edward M. Southern (1), can be used to detect ...commonplace, researchers used restriction mapping to investigate DNA sequence content by comparing the fragments...

...membrane-impermeant. With each cell division, the (invariant) amount of dye is reduced per cell (Figure 4a)...Harvard Medical School. His projects include investigating negative regulators of T cells and cytokine ...

...march is meant to inspire scientists to be more involved in their communities (something already encouraged...mathematical constant “pi” supports scientists getting involved in politics. Disclaimer: The views presented ...

...delivered through transfection of naked DNA. Other investigators who had been working to develop high efficiency..., which is particularly well suited for strand invasion, a key step in the homologous recombination pathway...

...Traditionally, delivery is achieved through the use of invasive methods, limiting researchers to accessible, spatially...Gradinaru, V. (2017). Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous... Gradinaru, V. (2022). Engineered AAVs for non-invasive gene delivery to rodent and non-human primate ...

... fragment library of phage DNA This first step involves digesting phage genomic DNA into 1-3 kb (approximately...fragmentation strategy is preferable. Nebulization involves forcing DNA through a small opening using compressed...

... likely due to the fact that PBx relies on the inverted terminal repeats (ITRs) found on the piggyBac™... Grégoire and M. Kmita, “Recombination between inverted loxP sites is cytotoxic for proliferating cells...

...working in a rural health clinic in Ghana. My role involved teaching the nursing students and community health... future supervisors were keen for me to become involved with many different aspects of the company and...

...to perform functional screens to identify genes involved in a phenotype of interest. This blog post will... is a very low-efficiency process, and usually involves the need for single cell cloning and subsequent...

...How are their organizational skills? Are they involved with colleagues and the department? Are they ...transferable skills by attending career events or being involved in a grad or postdoc association? Will you learn...