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Showing: 201 - 220 of 425 results

  1. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...with AAV sgRNA delivery make the SPH mouse an attractive tool to probe gene-function relationships in ...cell populations makes Cre-dependent SPH mice an attractive alternative. Whatever your application, first...

  2. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...preparations are purified by iodixanol gradient ultracentrifugation and concentrated to purity and titers adequate...vector, incubated for 72 hours, and genomic DNA is extracted. Primers and probes targeting integrated copies...

  3. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...cells in Lennox LB (10 g/L tryptone, 5 g/L yeast extract, and 5 g/L NaCl) or on LB-agar plates containing...intraperitoneal doxycycline injections to enable their tracking with ultrasound imaging (Fig. 2d). Creating a ...

  4. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ... circular corridor apparatus using a camera or tracking software. Locomotion is a highly complex process...PMC6333924. Howerton, Alexis R., Alison V. Roland, and Tracy L. Bale. "Dorsal raphe neuroinflammation promotes...

  5. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...mitochondria, lysosomes or they are secreted into the extracellular milieu. Many of the resident proteins within...space of mitochondria and chloroplasts, the extracellular milieu, and gram negative bacteria periplasm...

  6. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    .... For example, mouse behavioral geneticists may track the motion of multiple animals on different occasions...repositories emerge that allow efficient searching and extraction of information. Looking a little further ahead...

  7. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ... model organisms is that they are genetically tractable. Mice can be easily manipulated with tools like...al. "Efficient gene transfer in C. elegans: extrachromosomal maintenance and integration of transforming...

  9. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...Formally, a government can be defined as a social contract citizens enter into in order to protect their ...administration tells EPA to freeze all grants, contracts The Guardian: Trump to scrap Nasa climate research...

  10. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...proteins in both prokaryotic and eukaryotic cell extracts (Gagoski et al 2015). He has also constructed ....2 (2016): 93-106. FREQ-Seq To help scientists track the frequencies of specific alleles in microbial...

  11. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ... the separated guide RNA components (crRNA and tracrRNA) for maximum Easi-CRISPR efficiency. Easi-CRISPR...mCherry etc.), recombinases (Cre, Flp etc.), and tetracycline drug induction systems (ttA or rtTA) are between...

  12. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...monomeric version of the plasmid. Linearize, gel extract, and ligate the plasmid If you are having difficulty...linearized plasmid on an agarose gel. You can then gel extract the linearized band, ligate the plasmid, and re-transform...

  13. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...storyline involves the resurrection of dinosaurs by extracting the DNA from fossilized amber and supplementing... C (2000) Hugo De Vries: from the theory of intracellular pangenesis to the rediscovery of Mendel. Comptes...

  14. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...Anti-Cas9 Western blotting was performed on whole-cell extracts from stable pools of A549 cells transduced with...Anti-Cas9 Western blotting was performed on whole-cell extracts from the initial stable cell pool (pool) and from...

  15. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ... found in plants makes traditional knockouts unattractive from a practical perspective. Therefore, techniques...through the fluorescent reporter and manually extracted. This process is tedious and time consuming, as...

  17. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...include focused ultrasound (FUS), intracorporeal (IC), intracerebroventricular (ICV), intramuscular (IM), intrathecal...

  18. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...unique tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many of which ...Nucleic Acids Res. 1996. PubMed PMID: 8692687 Tracking autophagy in yeast with Rosella, a pH-sensitive...

  20. CRISPR 101 eBook...en español

    Type
    Blog Post
    Published
    Jan. 18, 2024, 2:15 p.m.
    ...excited! After receiving the translation, Addgene contracted with a professional scientific translator, L....
Showing: 201 - 220 of 425 results