...This post was contributed by ATCC Outreach Scientist Nick Amiss. Cell culture in the present day Cell...major cell lines on public databases. Image attributions (from top to bottom) Database Center for Life...culture outlined in this article. Now many journals strictly require evidence of cell line provenance and identity...Nature Publishing Group recently reiterated its strict stance on the matter. Funding bodies such as the... at LGC Group, London, which is the European distributor for ATCC. He studied biochemistry at Warwick ...

...This post was contributed by guest blogger, Stephanie Hays, a scientist with a passion for photosynthetic...States government? Politics and science are intrinsically linked: governments exist in reality and science...said, government must have the same fundamental attribute that enables science: “humility in the face of...supporting many different positions. It would be detrimental for them to be homogenized or thought of as just...Scott Pruitt says carbon dioxide is not 'primary contributor' to global warming The New York Times: With Trump...

...DNA, important when trying to cut with certain restriction enzymes (ex: ClaI or XbaI) dcm DNA cytosine...DNA, important when trying to cut with certain restriction enzymes that are methylation sensitive. dnaJ...relA1 Relaxed phenotype, mutation eliminating stringent factor Allows RNA synthesis in absence of protein...plasmid maintenance, blue/white screening, partly restriction-deficient; good strain for cloning repetitive...methylated, allows for methylation sensitive restriction enzymes to cut the plasmid after preparation....

...labs have deposited plasmids to Addgene and we distribute over 130,000 plasmids in 2014. Thus, we are in...on CRISPR technology, for example, the authors Patrick Hsu, Eric Lander, and Feng Zhang acknowledge the...accelerated through a combination of open-source distributors such as Addgene, as well as a number of online...with data comparing Addgene global deposit and distribution trends for Zinc Finger, TALE, CRISPR, and SynBio...

...announce that we recently acquired the ability to distribute plasmids with the piggyBac™ transposon. These...piggyBac™ transposase (available from a licensed distributor) allow you to quickly transfer a DNA sequence...transposon vector to one of many TTAA sequences distributed throughout the genome. We encourage you to deposit...expand this useful resource. While Addgene cannot distribute plasmids with the piggyBac™ transposase itself...from the folks at Transposagen. This post was contributed by Amar Singh and Mathew Goodwin from Transposagen...

...preparation for our upcoming affinity reagent distribution launch - and we're excited to say our long naming...to help us with our upcoming affinity reagent distribution launch! There was only one problem - we didn't... biggest discovery yet: Addgene will soon be distributing affinity reagents. Name Our New Antibody Friend...luck and happy naming!   Fig. 2: The mascot trio!     ...

...This post was contributed by Kartik Lakshmi Rallapalli, a graduate student at the University of California...manipulate it. The term genetic engineering itself is attributed to SciFi writer Jack Williamson who used it in... Zinc finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding...template.  Each zinc finger recognizes a nucleotide triplet and when several ZFNs are strung together, it can...the ZFNs, but instead of recognizing nucleotide triplets, the components recognize individual nucleotides...

...This post was contributed by guest blogger Eleanor Wang, a Research Specialist in Patrick Hsu's lab at ...problems. Biomedical research, for instance, contributes heavily to medical practice, and understanding... and applied. The current pandemic serves as a striking example of the applicability and importance of...Eleanor Wang is currently a Research Specialist in Patrick Hsu’s lab at UC Berkeley. She will be starting ...

...This post was contributed by guest blogger Søren Hough, a Biochemistry PhD candidate at the University...mismatch along the length of the gRNA given the stricter sequence requirement at the terminal 3’ PAM site... Dillard, and Ayokunmi Ajetunmobi for their contributions to this work. Søren Hough is a Biochemistry ... mismatch cleavage assays to detect mutations triggered by engineered nucleases." G3: Genes| Genomes| ...PMID: 27087594. 16. Tycko, Josh, Vic E. Myer, and Patrick D. Hsu. "Methods for Optimizing CRISPR-Cas9 Genome...

...scientists at 5,200 institutions in 82 different countries. We continue to ship out more plasmids each year...beginnings in 2004 to today, you can see how plasmid distribution has grown. Additionally, more and more scientists...research. *2015 total reflects the number of distributed plasmids as of March 26, 2015. How does Addgene... our depositors to allow us to help store and distribute their plasmids. The partnerships and collaborations...

...SaCas9 genome editing plasmids String assembly gRNA cloning (STAgR) String assembly gRNA cloning (STAgR)...studied Cas12b nuclease from Alicyclobacillus acidoterrestris (AacCas12b) exhibits optimal DNA cleavage at...STAgR), developed by Stefan Stricker’s lab, is a single step gRNA multiplexing system that generates vectors...

...component of Golden Gate and GreenGate are type IIS restriction endonucleases. They are like other endonucleases... should in principle work in any plant species. Tricks of the trade Lohmann does have one special tip ...their own lab: be very careful with the type IIS restriction endonuclease of choice, BsaI, which can be very... will get busy with GreenGate, producing and contributing more modules into the public domain in the process...

...cells that have been infected with a microbe, to trigger immune cascades that clear these infections. Anything...letters and be able to guess whether or not that string of letters is a part of the word you’re searching... that you want the antibody to recognize. This triggers an immune response within the animal, and its ...antibodies (i.e., immunofluorescence), or by a colorimetric reaction facilitated by an enzymatic reaction... antibodies for neuroscience research from the Trimmer lab ...

...detergents such as triton-x, saponin, or tween. Take care when using strong detergents like triton-x and tween...fluidics system they pass through a flow cell which restricts the size of the stream and forces the cells to...cell expressing low levels of the protein. The electrical system converts the fluorescence intensity to...you would need a way to determine the relative contribution of yellow and orange from FITC and that from...

...This post was contributed by Doug Richardson, Director of the Harvard Center for Biological Imaging and...focus fluorescence from reaching a detector or restricting fluorescence to specific excitation volumes to...reduction in signal intensity. 2-Photon microscopes restrict excitation (and bleaching) to a single focal point...microscopy uses a number of optical and chemical ‘tricks’ to turn subsets of fluorescent molecules on and... a large axial range. 2-photon imaging, which restricts excitation and photobleaching to the focal plane...

...various grades of plastic lumber and distribute it to industrial and residential markets. Rethinking our...this, we can use the ACT label, which is an eco-nutrition label for laboratory products that emphasizes ...typically is destined for a landfill. However, Triumvirate Environmental developed Red2Green, a program ...

...in the same vector backbone with very similar restriction enzymes. You can essentially cut and paste quite...mind that a very simple sequential or double restriction enzyme digest will open up possibilities exponentially...these vectors to the scientific community and contribute in a way that everyone could utilize what we ...really the one who had been very adamant that we distribute to as many researchers as we could, and Addgene...

...programs either. But with a little Googling and some trial and error, I found some free design software that...number of drawing tools, so it’s harder to draw intricate figures, like a brain or a mouse. Vectr Vectr ...and digital articles where it’s easier to give attribution, than for journal publications. Google Image ...includes images that can’t be reused without attribution, despite filtering for images labeled for reuse...

...let us know in the comments. Note:Marcy Patrick contributed to the writing of this article. ... Short segment of DNA which contains several restriction sites allowing for the easy insertion of DNA....utilize one of a variety of cloning methods (restriction enzyme, ligation independent, Gateway, Gibson...

... al., 2020). A transducer complex consists of a trimer of three subunits: alpha, beta, and gamma (Gα, ..., Gβ, and Gγ), collectively known as a heterotrimeric G protein. This complex converts a signal received.... When inactive, these subunits are found in a trimeric complex bound to the intracellular surface of ...called BRET2. Figure 1: TRUPATH measures heterotrimeric G protein dissociation by bioluminescence resonance...