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  1. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...which will bind and light up a tagged protein for imaging. Given its popularity and widespread usage, a proteolysis-targeting... avoid AID tags. If your project also involves imaging at some stage, then choosing a HaloTag could pay...

  2. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...fluorescent signaling in the presence of dsDNA. Image courtesy of: Ygonaar    Taqman is more sensitive...thousands of PCR reactions using Taqman chemistry (see image, left, of droplet immersion technique, courtesy ...

  3. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ... Figure 1: Mobile-CRISPRi's modular components. Image from Peters et al., 2019 with permission.   ...recipient bacteria with an integrated copy of CRISPRi. Image from Peters et al., 2019 with permission.    ...

  4. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...confocal microscope. You’re trying to get better images for a paper and she’s trying to get some data for...manuscript is more important to you than collecting your images this week. Even though three examples depend on...

  6. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ... extend far beyond the laboratory and seriously damage public trust in scientists, experiments involving...technologies with care and humility. Nothing would damage that trust more than an accidental gene drive release...

  7. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...Fluorescent proteins and their applications in imaging living cells and tissues”  Physiol. Rev. 90:1103...et. al. (2011) “Modern fluorescent proteins and imaging technologies to study gene expression, nuclear ...

  8. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...   Figure 2: Structure of a retrovirus. Image re-used from Dong & Kantor, 2021 under CC-BY license...   Figure 3: The life cycle of a retrovirus. Image courtesy of Jeremy Luban, MD. Figure 3 depicts...

  9. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...fluorescent label, which can reduce the potential image resolution of super-resolution fluorescence microscopy...Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex...

  10. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...would help you do the job as described. Let’s imagine you’re applying to a job that requires ddPCR skills...answer to that is what goes into your cover letter. Imagine you’re applying to a job with a lot of file management...

  11. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    .... Steps 1-1 and 1-2 can be done simultaneously. Image from Li et al., 2021.   The updates involved...gene dosage on N-acetylglucosamine concentration. Image from the Yang lab.   In conclusion, the MUCICAT...

  12. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...-inducible Cre not only halted the neurological damage but completely reversed it, regardless of age or...corrected by CRISPR/Cas9 in wild-type human iPSCs. Image modified from Le et al., 2019. Gene editing ...

  13. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...with mRNA and charged tRNAs to build a peptide. Image from Mariana Ruiz Villarreal. By the late 1980s... codons are under expressed compared to others. Image from Hiss et al., 2017. Most commonly, scientists...

  14. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...knock in, base edit, activate, repress, and even image your favorite gene. With so many Cas9 reagents available...target strand based on the sequence of the pegRNA. Image from David Liu.   Activation and repression...

  15. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ... appear darker than full vector particles. This image shows that the vast majority of the vectors consist...particles (black arrowhead). Scale bar = 100nm. Image courtesy of David Bell and Svetla Stoilova-McPhie...

  16. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...groups have utilized dCas13 fusion proteins for imaging, tracking, modulating splicing, and regulating ...fluorescent fusion protein dLwaCas13a-NF for in vivo RNA imaging. In short, the Cas13a molecule brings a much anticipated...

  17. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...schools. I will emphatically deny that I have a magic formula to guarantee a reader the faculty job of...optimization of fluorescent proteins,  and live cell imaging approaches. Erik is also an avid long distance ...

  18. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...now to capture interesting key results, striking images, creative ideas, and engaging stories as you encounter...supportive audience? 7. Hone Your slides Use big images that are easy to see (and for heavens sakes, label...

  19. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...paper. Pretty much like an actual sandwich! S'mores image from Evan Amos.   After the transfer, some areas...chemiluminescence, is detected on x-ray film or with specific imaging devices. In most cases, the primary antibody, which...

  20. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...5, 6, 8, and 9 in two different brain regions.  Image modified from Aschauer et al., 2013, Figure 1. Used...both oligodendrocytes (Olig2) and neurons (NeuN). Image from Griffin et al., 2019, Figure 4. Used under ...
Showing: 181 - 200 of 387 results