...month including a first of its kind for the Addgene blog - Addgene Software Engineer Kris Shamloo wrote a ... Read All Our Editor's Choice Blog Posts The Addgene Blog has had quite the up-tick in views over the...constructs. The second post was contributed by Addgene Scientist Mary Gearing and discusses a newer, CRISPR-based...advice on how to give a great science talk from Addgene Executive Director Joanne Kamens. I highly recommend...

...Professional Organization Resources on Addgene.org Check out Career's at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting... by some colleagues as the “cross-team guy” at Addgene, an unofficial title that I happily embrace (and...Management Team that steers the organization. As an Addgene vet familiar with the expertise and skills of many...possibly understand every nuance of how kits work at Addgene, but with the help of these people and others, ...existing. Here are a few types of teams that exist at Addgene - team types that could exist anywhere in science..., they must work in sync to get tasks done. At Addgene, the Scientist Team is responsible for guiding ...

...plasmids (Addgene #104102 - #104105) using in utero electroporation or with AAVs (prepared with Addgene #104110...topic page Additional resources on Addgene.org Learn about Addgene's viral service See our fluorescent...SNAP-tag, Halo-tag, and CLIP-tag (Kohl et al., 2014) (Addgene #104106 - #104108) (Figure 3) that can be introduced...PubMed PMID: 24746791. Additional resources on the Addgene blog Read about Brainbow Learn about using AAVs...fluorescent protein collection Find AAVs at Addgene ...

.../nature18308   Additional resources on Addgene.org Addgene’s General Use Antibodies Antibody guide ...applications. You can find FLAG antibodies on Addgene’s catalog including a mouse monoclonal (M2) as well...clone used today! Goat, chicken, mouse, or rabbit? Addgene has you covered with monoclonal antibodies to Myc...both cleave after the DVPD sequence. Check out Addgene’s monoclonal antibodies (mouse, rabbit, and chicken... antibodies or guidance on designing a GFP tag Addgene has you covered!   Potential tag issues Tags have...Antibody protocols Additional resources on the Addgene blog Antibodies 101: Epitope availability Plasmids...

...Here at Addgene we’re getting into the holiday spirit by kicking off our annual #DeckTheLab contest. ...and tweeting it with the tags #DeckTheLab and @Addgene. We’d love to see how you’re decorating your lab...lucky winner? Happy holidays from all of us at Addgene! We look forward to seeing how you #DeckTheLab!...

...on the Addgene blog Learn more about Blugene's make-over Get a how-to guide on the new Addgene search...the finalists will receive some newly designed Addgene swag. Have fun! Suggest a name for Blugene's new...their virus friend a gender neutral name. The Addgenies will choose up to four finalists and we’ll ask...

...plasmids from this publication at Addgene. Additional Resources on the Addgene Blog Read More about Fluorescent...cells! This technique was developed in the lab of Addgene depositor Stanley Qi and used in Chen et al. to...colorful? CRISPRainbow is available as a kit from Addgene.   References 1. Chen, Baohui, et al. “Dynamic...PMC3918502. Find plasmids from this publication at Addgene. 2. Ma, Hanhui, et al. “Multicolor CRISPR labeling...PMC4364232. Find the plasmids from this publication at Addgene. 3. Ma, Hanhui, et al. “Multiplexed labeling of...PMC4864854. Find the plasmids from this publication at Addgene. 4. Kiani, Samira, et al. “Cas9 gRNA engineering...

...how they will use plasmids and viral preps from Addgene to further their work. The Association for Creatine...channel. With the BeHeard award, the ACD will use Addgene’s RESOLUTE plasmid collection to perform a functional...keratinocytes. “We hope to use CRISPR libraries from Addgene to set up assays to see what molecules or proteins...Trolle et al., 2016). Using lentiviral vectors from Addgene, the lab will express these candidate genes in ...candidate genes in euploid iPSCs. “The BeHEARD Addgene reagents will facilitate our aims to correct X ... these pathways using biosensors as well. “The Addgene catalog would allow us to enhance our biosensor...upon and depositing their modified constructs to Addgene. Congratulations Terry Hébert! Members of...

...plasmids from this paper at Addgene. Additional Resources on the Addgene Blog Check out our CRISPR Featured...PMID: 29070703. Find plasmids from this paper at Addgene. Abudayyeh, Omar O., et al. “RNA targeting with...PMID: 28976959. Find plasmids from this paper at Addgene. Abudayyeh, Omar O., et al. "C2c2 is a single-... PMC5127784. Find plasmids from this paper at Addgene. East-Seletsky, Alexandra, et al. "Two distinct...PMID: 27669025. Find plasmids from this paper at Addgene. Gootenberg, Jonathan S., et al. "Nucleic acid... PMC5526198. Find plasmids from this paper at Addgene. East-Seletsky, Alexandra, et al. "RNA Targeting...PMID: 28475872. Find plasmids from this paper at Addgene. Abudayyeh, Gootenberg, et al. “A cytosine deaminase...

...and tactics for getting the plasmids he wanted. Addgene makes it so much easier to share your constructs...constructs from lab to lab. Promega supports the Addgene mission statement: Accelerate research and discovery...from the repository. We encourage people to go to Addgene to get new innovative tools. Afterall, isn’t science...constructs for GpNLuc and OgNLuc are available through Addgene (Cat.# 70185 and 70186, respectively). Antares...The construct for Antares is available through Addgene (Cat. # 74279). Enhanced Nano-Lanterns  Suzuki... Nano-Lantern constructs are available through Addgene: The Turquoise (CeNL; Plasmid #85199), Yellow (... explants. This construct is available through Addgene (Plasmid #83926). Live-Cell Voltage Sensor  Ingaki...

...find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources...advantages for cellular delivery. Get the plasmids at Addgene Sc++ is a codon-optimized, engineered Streptococcus...has a broad 5′-NNG-3′ PAM. Get the plasmids at Addgene The Human GlycoGene CRISPR Library is a new lentiviral...to cellular glycosylation. Get the library at Addgene CasRx is a RNA targeting Cas system that can be... Drosophila melanogaster. Get the plasmids at Addgene The Cas Hybrid for Multiplexed Editing and screening... with alternative exons. Get the library from Addgene New from the viral service By Jason Nasse We...18,000 human genes. Find the lentiviral preps at Addgene hSyn1-SIO-stGtACR1-FusionRed is a Cre-dependent...

....2024.100519 More resources from Addgene Plasmids 101: Modular Cloning Addgene's CRISPR Guide Plasmids 101: ...been looking for just the right CRISPR vectors on Addgene and found instead ones that were… pretty close,...Fragmid components are available for request on the Addgene website, both as individual plasmids and as the...component available on the Fragmid website, or through Addgene, has worked well in the hands of the Doench lab...the ID to look up the component plasmids on the Addgene website. Step 4: Naming your assembled plasmid...Fragmid component (which you can also deposit with Addgene!) We’re excited to help the Doench lab share this...

...Many of these promoter systems are available at Addgene! Chemically inducible promoters Chemically regulated... Promoter Subtype Promoter Example Activator Addgene Plasmid/Kit Alcohol-regulated AlcA promoter...the promoter, thereby activating transcription. Addgene depositors have developed heat shock-inducible ...allowing reporter gene transcription to take place. Addgene also has yellow flame light-regulated two component...writing of this article. Additional Resources on the Addgene Blog: Plasmids 101: The Promoter Region Plasmids...Tetracycline (Tet) On Expression Systems at Addgene Cre-lox Guide and Plasmids     ...posts from our Plasmids 101 series Resources on Addgene.org: Inducible Promoters for Bacteria Tetracycline...

...plasmids from this paper at Addgene. Additional Resources on the Addgene Blog Learn about Photoactivatable...proteins. To make luminescent imaging a reality, Addgene depositor Takeharu Nagai and colleagues at Osaka...ready-made Nano-lantern biosensors are available from Addgene! References Saito, Kenta, et al. “Luminescent ...: PMC3535334 Find plasmids from this paper at Addgene. Takai, Akira, et al. “Expanded palette of Nano-lanterns...: PMC4394297 Find plasmids from this paper at Addgene. Suzuki, Kazushi, et al. “Five colour variants...Use Rosella to Study Autophagy Resources at Addgene.org Check Out All Our Fluorescent Protein Resources...

...’t. Try out the new Addgene search engine! Additional resources from the Addgene blog Learn about other...other features on Addgene's website Find a step-by-step guide to using Addgene's new search Read to learn...posts or reagent items.  The technology behind Addgene’s new search  Like millions of other websites, we...data in particular and our deep understanding of Addgene’s customer’s needs. Categories We created a categorization...effort and cross-team collaboration (thanks to all Addgenies involved). The new homepage and navigational elements...

...plasmids from this publication at Addgene. More Resources at Addgene: Check out more synthetic biology... in other fields, including synthetic biology. Addgene depositors Christopher Voigt and Jeffrey Tabor ...PubMed. Find the plasmids from this publication at Addgene. Synthetic biology: engineering Escherichia coli...PubMed. Find the plasmids from this publication at Addgene. A synthetic genetic edge detection program. Tabor...PubMed. Find the plasmids from this publication at Addgene. Multichromatic control of gene expression in ...

...touch! Resources at Addgene Read Our Posts on Networking Get Career Advice from Addgene Bloggers Learn How...and some of the great biological societies that Addgene has had the opportunity to work with at Freshers...outside academia. Inspiring future scientists At Addgene, we’re incredibly glad to see these biological ...society or event which would benefit from some Addgene support? Please get in touch, as we aim to support...

...multiple protein molecules (and also available from Addgene!) Since the signal level provided by SunTag is ...pantheon of useful CRISPR/Cas9 techniques, we at Addgene are excited to see what’s coming next! References.... Find the plasmids from this publication at Addgene. Dynamic imaging of genomic loci in living human.... Find the plasmids from this publication at Addgene. A protein-tagging system for signal amplification.... Find the plasmids from this publication at Addgene. ...

...researchers as we could, and Addgene is a wonderful vehicle for us to do that.” At Addgene, we are equally excited...is available in an easily searchable format on Addgene’s Michael Davidson Fluorescent Protein Collection...collection with over 300 backbones alone,” said Addgene’s Lianna Swanson, who has been working with members...the scientific community," says Joanne Kamens, Addgene's Executive Director, "and to promote the great ...

...around the Addgene office as we peruse the #DeckTheLab submissions. We asked all of the Addgenies to pick...MerryChristmas #happyholidays2018 #DeckTheLab @Addgene @joachimgoedhart @SAbiega @EikeMahlandt @Saskia_deMan... @EmoryChem lab office looking festive 🎄🌟🎁 @Addgene #DeckTheLab pic.twitter.com/bDRqAKrWHz — Dyer Lab...winners will receive a holiday care package from Addgene. We hope all of our entrants had fun decorating... agar art from Nika Heijmans stood out to the Addgenies. Happy holidays!🎄☃️ More #agarart from our ...If you’re feeling as chilly this winter as the Addgenies are in Massachusetts, have a look at the Dyer ...