Skip to main content
Addgene
Showing: 181 - 200 of 425 results

  1. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...and requires only one RNA rather than the two (tracrRNA and crRNA) needed by Cas9 for cleavage. Now, two...NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED TO DEMONSTRATE...

  2. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ... produce low levels of virus and can be used to track carryover. Functional titer Functional titer is ...Learn How Lentivirus Has Been Used for Lineage Tracing Additional Resources on Addgene.org Learn How...

  3. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ... violet light and (2) exposure to increased intracellular calcium. It has been applied to mark active ...Whether you’re studying the brain, muscles, or intracellular landscape, remember: Keep Ca+2lm and Ca+2rry...

  4. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...near point mutations to create 3-base (or longer) tracts of edited bases. DNA mismatch repair (MMR) is less... protect the end of the pegRNA. Adding 3′ polyU tracts to the end of pegRNAs (but not epegRNAs) can improve...

  5. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...China’s Ribobio, the illustrious speaker list attracted a host of sponsors based all over the world. With...Using the Tet-on system, his lab has created a tetracycline cas9 inducible knock-out system. His lab is ...

  6. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...our ideas got pretty good. We started doing extracurricular research on some of them to see how feasible...SynBio Axlr8r We limped along with very little traction for quite a while, and even had some serious self-confidence...

  7. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage numbers Keep an eye on the ...

  9. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...business suits discussing multimillion dollar contracts behind closed doors. Fig. 1: The mythical... committees as a tenured professor or signing contracts when working for a business development team, ...

  10. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...photocleavable gRNAs within seconds. A CRISPR-based lineage tracing tool captures rare clones that represent 0.001%...where gRNA expression is induced by anhydrous tetracycline. Three new anti-CRISPRs were identified that...

  12. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...is typically purified using methods like ultracentrifugation, while lentivirus supernatants are centrifuged...collection 2–4 days 5–10 days Purification Ultracentrifugation (AAV); centrifugation (lenti) Centrifugation...

  13. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...centrifugation (e.g., iodixanol) using a high-speed ultracentrifuge. During the spin, the virions will form a band...ddPCRAAV Purification by Iodixanol Gradient UltracentrifugationAAV Production in HEK293 Cells...

  14. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...sequences (blastn)” program for blastn so that you can extract practically every single reliable base pair from...longer alignments to compare with your sequencing trace file. Unlike megablast, the regular blastn program...

  15. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...1993). But why did this technique fail to gain traction? It is likely that both vector amplification with...performing multiple modifications will require ultracompetent (109 CFU/μg) cells. Primer design Using specific...

  16. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ... and regulations impacting grant management, we track grant opportunity deadlines, and we ensure that ...questions when you’re negotiating a new job or a new contract. Connect with other grant professionals on LinkedIn...

  18. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ... the transformation and gene expression can be tracked. During a transient leaf transformation, the engineered...was successful at all. Once we were on the right track, we switched to luciferases to more easily quantify...

  19. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...with AAV sgRNA delivery make the SPH mouse an attractive tool to probe gene-function relationships in ...cell populations makes Cre-dependent SPH mice an attractive alternative. Whatever your application, first...

  20. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ... to tackle it. It’s easy to get distracted by an email or sidetracked by a colleague. To innovate, you...
Showing: 181 - 200 of 425 results