...immune response from the body. Several factors contribute to the immunogenicity of a drug, including the... body recognizes a drug as a foreign antigen, triggering a humoral immune response. ADAs can inactivate...one of the more common open-access tools, while EpiMatrix is a commercially available algorithm to evaluate...in silico assessments, as well as analyze the contributing factors that in silico prediction algorithms...monocytes. PBMCs can be further differentiated into dentritic cells or macrophages through cytokine induction...

...In addition to single plasmids, Addgene also distributes pooled plasmid libraries containing hundreds,...versatile offerings! We recently re-amplified our distribution stock of the Brunello Human gRNA library, and...processes we use to ensure high-quality library distribution. You can also use these tips as a starting point... scaled to 1, to create a “cumulative wealth distribution” curve, or Lorenz curve. By calculating the ...gain a sense of the uniformity of the library distribution, with an AUC of 0.5 representing perfect uniformity...

...Light-mediated block of synaptic transmission Article contributed by Klaus Wanisch  Listen to the optogenetics ...imaging of microbes in mammalian hosts Article contributed by Lukas Morgan  Listen to the acoustic reporter...and create ultrasound contrast. At a very low volumetric density and with a resolution of less than 100...mechanisms of miRNA-mediated gene regulation Article contributed by Michael Lemieux  Listen to the miRNA reporter...vivo using the CRISPR-Cas9 system CHIME Article contributed by Aliyah Weinstein  Listen to the CRISPR podcast...

...This post was contributed by Mary Tamer from BioBuilder, an innovative nonprofit bringing the study of... new for themselves and when they effectively contribute as part of a team.” As a seasoned educator, Kuldell...almost every state in the U.S. and in more than 30 countries around the world. Snapshots of the BioBuilder ...open-access curriculum begins with students reading comic strips and watching animations that present a specific... lab materials and reagents such as droppers, centrifuge tubes, circuit parts, and lab-strains of bacteria...

...presentation, starvation or the use of caloric restriction mimetics trigger autophagy and, in a mouse model led ...deposit and distribution service! References: 1. Pietrocola, Federico, et al. "Caloric restriction mimetics...from a state of chronic hepatitis caused by hypernutrition and alcohol consumption. Chronic inflammation...

...collaborative group, she also joined early on and contributed her expertise in developing reproducibility workshops...Joanne Kamens, Addgene also had the opportunity to contribute to this open science collaboration. The first...workshop at the conference, but because each contributor made the materials accessible, anyone could present...present the material. For example, Addgene’s contributions by Tyler Ford included reagent sharing and were... upcoming conference or you’d like to join or contribute to this collaboration, please check out the official...

...worry about and that is something to recognize! Distributing plasmids, viral service, and more For the ...about succeeding at our core mission. Plasmid distribution is amazingly still growing at >9% every year...since 2005. We’re meeting the challenges of DNA distribution to ensure scientists everywhere can access plasmids... it. Almost 10,000 viral vector samples were distributed in 2018 and we are poised for more offerings....Addgene. There’s so much potential for service and contribution! Leadership On an organizational level, ...

...He has recently developed a third-generation intensiometric glutamate sensing fluorescent reporter (iGluSnFR3...to identify cell populations in the brain that contribute to the development of behavioral signs of opioid...connectivity patterns of these cells and the functional contribution to a variety of behaviors related to addiction...expressed in a mammalian cell line engineered to be electrically excitable. The mutants are co-expressed with...membrane voltage,” says Tian. Figure 5: Citrine-tagged voltage indicator expressed in mouse cortex...

...The post was contributed by guest blogger Londa Schiebinger, PhD, Hinds Professor of History of Science...the US Women’s Health Initiative Hormone Therapy Trial, for example, found that the study saved lives. ...was $35.2 billion (or $140 per $1 invested in the trial). The goal of gendered innovations It is crucially...research is minimal compared to that of human clinical trials. And, as Janine Clayton points out, late-stage ...because sex was not analyzed) may harm humans or contribute to failures in drug development – costs far greater...

... by a variety of methods such as ultracentrifugation, centrifugation on a sucrose gradient, spin columns...producing and using lentivirus and offer some tips and tricks for overcoming these hurdles. Talking 'bout my...relatively inexpensive and does not require an ultracentrifuge, PEG will precipitate all proteins in a solution...high-titer lentivirus preparations without ultracentrifugation. Sci Rep. 2015; 5:13875. PubMed PMID: 26348152...

...This post was contributed by Patrick Miller-Rhodes from the University of Rochester Medical Center.  ...guest blogger Patrick Miller-Rhodes from the University of Rochester Medical Center! Patrick is equal parts...in large enough quantities (and the correct stoichiometric ratios) to generate useable data. What’s more...

...dependencies they identified and their associated ancestries. While there were some exciting relationships...Dependency Map) than lines from European or East Asian ancestries. These SNPs, however, were not accounted for ...racial bias. It is easy, even unintentionally, to attribute to an ancestry group what is actually an artifact...and references References Germline variation contributes to false negatives in CRISPR-based experiments...experiments with varying burden across ancestries. Sean A Misek, Aaron Fultineer, Jeremie Kalfon, Javad Noorbakhsh...

...generally referred to as Antigen Retrieval. I chose not to get into antigen retrieval here because it’s not quite...permeabilization reagents are detergents, such as Triton X-100, Tween-20, or saponin. Each detergent has...membrane-associated proteins in place. In contrast, Triton X-100 and Tween-20 are examples of non-ionic detergents...Permeabilizing with a non-ionic detergent, like Triton X-100, can lose membrane-associated proteins, but...

...they are transferrable across most, if not all, industries and work environments. This is why they are called... bathroom mirror pep talk, forgotten and then retrieved your lucky socks, persevered through a nerve wracking...potential teammates want to be assured that you will contribute openness, clarity, approachability, and professionalism...interview, check out this article from The Muse. Kai Strickland is a former Customer Support and Operations Manager... Kai Strickland...

...This post was contributed by Katherine Rogers, a postdoctoral researcher at the Friedrich Miescher Lab...processes—not just development—are available from strain distribution centers in the US, Europe, and China. In addition...pathways—such as those regulated by one-eyed pinhead—contribute to the “sculpting” of tissues and organs during...Katherine W. Rogers is a postdoctoral researcher in Patrick Müller’s group at the FriedrichMiescher Laboratory...El-Brolosy, Mohamed A., et al. "Genetic compensation triggered by mutant mRNA degradation." Nature 568.7751 (...

... Weakly electric fish are excellent systems to study for convergent evolution because electric organs ...leave them to their own devices. Chytrid fungi (Lillian Fritz-Laylin) Chytrid fungi are useful for the study...but there are few genetic tools currently for terrestrial frogs. Cnidarian-Dinoflagellate symbionts (Virginia...

...It is also worth noting that plasmids can be symmetrically incompatible, meaning that both plasmids are... from the cell with the same probability, or asymmetrical, where one plasmid lost from the cell at a higher... higher rate than the coresident plasmid. Asymmetric plasmid loss occurs for several reasons, including...high copy incompatible plasmids that lead to asymmetrical plasmid loss of the usually large, low copy ...: 15916596 Kamruzzaman M, Shoma S, Thomas CM, Partridge SR, Iredell JR (2017) Plasmid interference for...

...controlled by collections of neurons that are intricately wired into circuits through synaptic connections...weakly expressed. As these epitopes tags have no intrinsic visual signal, they cannot be detected directly...neurons. As compared to epitopes tags, FPs are intrinsically fluorescent and can therefore be used in live... interactions. Also, overexpression of FPs can trigger protein aggregation which is toxic for the cells...darkened chromophores. These probes are both intrinsically fluorescent and can be detected with highly ...

...This post was contributed by Kusumika (Kushi) Mukherjee, a Postdoc at Massachussetts General Hospital...patients with skin disorders for research and clinical trials, as it allows access to a larger number of willing...phenomenon has been recorded in past gene therapy trials and remains a safety concern in the clinical field...Furthermore, working with Sendai virus requires stringent biosafety measures, thereby increasing the costs...Using Serial Plating of Reprogrammed Cells by Centrifugation. Stem Cells Int, 2016. 2016: p. 1329459. PubMed...

... This post was contributed by Ryan Watkins, Professor at George Washington University and developer of...may also benefit, such as researchers in other countries or ot­her disciplines, are even less likely to...research from other disciplines, or from other countries, to explore new connections to our research. So...hours of video were watched each month, in 39 countries, and in over 54 languages. While most scientific...citation rates to measure the impact of their contribution. It offers a 5-step process and several examples...