... derived from Streptococcus pasteurianus  or the type II CRISPR3 module of Streptococcus thermophilus ...thermophilus (the popular Streptococcus pyogenes system does not work well in Caulobacter). To test their CRISPRi...

...SpyCatcher system, based on a protein isolated from Streptococcus pyogenes that locks itself together, to produce...they call SpyLigase (Spy comes from the “S” in Streptococcus and the “py” in pyogenes), capable of locking...links in the chain.  Addgene: What about the Streptococcus pyogenes protein domain made it ideal for this...

... Species/Variant of Cas9 PAM Sequence Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E...Neisseria meningitidis (NM) 3' NNNNGATT  Streptococcus thermophilus (ST) 3' NNAGAAW  Treponema ...

...control of CRISPR-Cas9. By combining AcrIIA4, a Streptococcus pyogenes Cas9 inhibitor, with the LOV2 photosensor...

...infection of certain tissues. However, packaging Streptococcus pyogenes (SpCas9) and a gRNA together (~4.2 ...Cas9 include the use of St1Cas9 (~3.3 kb) from Streptococcus thermophilus and a rationally-designed truncated...

...below.  Cas9 species PAM sequence ’) Streptococcus pyogenes (Sp) 3' NGG Staphylococcus aureus... Campylobacter jejuni (Cj) NNNNRYAC Streptococcus thermophilus (St) NNAGAAW Treponema denticola...

...deaminase (TadA) to a catalytically impaired Streptococcus pyogenes Cas9. They called this editor Target-ACE...

...types. Purpose  SpCas9 This plasmid expresses Streptococcus pyogenes (Sp) Cas9 from a Cbh promoter. Cbh ...

...system derived from Streptococcus pyogenes. An alternative Cas9, from Streptococcus aureus, is as effective...

...mesophilic (medium heat loving) bacteria, such as Streptococcus pyogene’s SpCas9. These Cas9 proteins don’t ...

...sequence targeted by the Cas9 nuclease. The Streptococcus pyogenes Cas9 (SpCas9) requires an NGG PAM to...

...species of bacteria. The stain colored one type, Streptococcus pneumoniae, while the alcohol used in the staining...

... the most frequently used Cas9, spCas9 from Streptococcus pyogenes, this sequence is 3’-NGG. But Cas9s...

...plasmid from the Zhang lab expresses Cas9 from Streptococcus pyogenes with an invariant sgRNA scaffold and...

... repression in the Gram-positive bacterium, Streptococcus pneumoniae. Robert Husson lab's M. tuberculosis...enzyme. They identified the Cas9 orthologue from Streptococcus thermophilus, Cas9sth1 as a robust and highly...

...conventionally used SpCas9 (derived from the bacterium Streptococcus pyogenes). Consequently, fewer genetic tools...

...Addgene Sc++ is a codon-optimized, engineered Streptococcus canis Cas9 nuclease that has a broad 5′-NNG-...

...meningitidis (Nme) (Pawluk et al., 2016). While Streptococcus pyogenes Cas9 (SpyCas9) is the most used and...

...Frederick Griffith used mouse models to prove that Streptococcus pneumoniae bacteria could be transformed from...

...looking at, let’s peek at unbound—“apo”—Cas9 from Streptococcus pyogenes.   Figure 2:  Crystal structure...