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CRISPR Guide

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Guide

...can enhance CRISPR specificity. SpCas9 (from Streptococcus pyogenes ) is the most popular Cas endonuclease...Sequences Species/Variant of Cas9 PAM Sequence* Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant...NNNNRYAC Neisseria meningitidis (NM) 3' NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola...