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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 48 results for: hyper

Showing: 1 - 20 of 48 results

  6. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...437-43. Tobias Dick Hydrogen Peroxide (H 2 O 2 ) HyPer biosensor to measure changes in intracellular hydrogen...hydrogen peroxide concentration Belousov Lab HyPer Plasmids Vsevolod Belousov Hydrogen Peroxide (H 2 O...Peroxide (H 2 O 2 ) Hydrogen peroxide biosensor HyPer7 Caveolae respond to acute oxidative stress through...H 2 O 2 ) Expresses hydrogen peroxide biosensor HyPer2 in plant cells Photosynthesis-dependent H 2 O 2...

  7. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...mobile DNA, called a transposon, that’s a bit hyperactive and likes to jump around from one location in... at rates similar to retroviral vectors. The hyperactive version of the SB transposase, SB100X, is ~100...transcription start sites, CpG islands, and DNaseI hypersensitivity sites. Like Sleeping Beauty, piggyBac has ...human cells similar to retroviral vectors. The hyperactive PB transposase (hyPB) is ~10-fold more active...transcription start sites, CpG islands, and DNaseI hypersensitivity sites. Tol2 is only active in vertebrates ...

  8. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...treated with sucrose or other osmolytes to induce hypertonicity display enhanced monoclonal antibody production...Shen, 2011). To our knowledge the effect of hyperosmolarity on AAV production yields had yet to be examined... and antibody production under conditions of hyperosmotic stress." Advances in animal cell biology and...18597326. 5. Shen, Chun Fang, and Amine Kamen. "Hyperosmotic pressure on HEK 293 cells during the growth ...

  10. Hot Plasmids - January 2023

    Type
    Blog Post
    ...constructs Anti-GFP [N86/8R] ready-to-use antibody Hyperfolder YFP CRISPR-Csm: A novel RNA targeting system...eLife.43322. PMID: 30667360; PMCID: PMC6377228. Hyperfolder YFP- a chemically stable tool for microscopy ... The Liu and Petsko labs recently engineered hyperfolder YFP (hfYFP), a FP well-suited for the demands...

  11. CRISPR Guide

    Type
    Collection
    ...spectrum of single-nucleotide variants (somatic hypermutation) in a larger editing window. These systems are...REPAIR , developed by the Feng Zhang lab , used a hyperactive adenosine deaminase that acts on RNA, ADAR2(E488Q...Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nature Methods . 13 (12)...

  12. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...lab screened many SB tranposase variants for hyperactivity in mammalian cells. Their efforts resulted in...Mates L, et al. Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable...

  14. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...Community Relations Director for Curiosity Collider (hyperlink https://curiositycollider.org/) , which she co-founded...and the Outreach Manager for Science Borealis (hyperlink http://scienceborealis.ca/)    ...

  15. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...autosomal dominant mutation in MYBPC3 that leads to hypertrophic cardiomyopathy. An adult male with a heterozygous...applicable in many situations. In the case of hypertrophic cardiomyopathy and other genetic disorders, ...

  17. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    .... https://pubmed.ncbi.nlm.nih.gov/24390935/ Hypercompact genome editor CRISPR-Casɸ By Melina Fan The ...toolbox with their discovery of CRISPR-Casɸ, a hypercompact genome editor from the Biggiephage clade of ...

  18. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...photoactivatable, optogenetic accuators to depolarize or hyperpolarize membranes. Traditional FRET reporters could ...depolarization increases the BRET signal whereas hyperpolarization decreases the signal. The authors additionally...

  19. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...results in efflux of chloride ions, subsequent hyperpolarization, and inhibition of action potential generation...spectrum as HR and also results in membrane hyperpolarization but unlike HR, this is achieved through hydrogen...

  20. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...spectrum of single-nucleotide variants (somatic hypermutation) in a wider editing window, and are thus well...
Showing: 1 - 20 of 48 results