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  1. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...fluorophore-conjugated streptavidin protein, which binds the primary antibody through the biotin-streptavidin interaction...detection. The advantage of this system is that streptavidin conjugates are commercially available for a ...fluorophore selection for panel design. Find streptavidin plasmids and biotin plasmids at Addgene!   ... two-protein labeling by utilizing(a) biotin-streptavidin binding and (b) antibody host species interactions...or protein-protein interactions, like biotin-streptavidin binding and antibody host species reactivity...
  2. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ... are tagged with streptavidin, the lateral flow strip first captures the streptavidin portion of the probe...biotinylated primers. These are then immobilized with streptavidin coated beads. Fluorescently labelled dCas9 complexes... concept to lateral flow strips that contain streptavidin which can bind to the biotinylated targets. ...
  3. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...egg-white protein avidin (or its derivatives streptavidin and neutravidin) is for all practical purposes...purification of biotinylated protein on blots with Streptavidin-phosphatase (Jackson ImmunoResearch, 016-050...
  4. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...mammalian cells with biotin ligase and monovalent streptavidin.” Nature Protocols 3.3 (2008): 534–545. PubMed...Biotinylation Guidelines Expedeon: The Biotin Streptavidin Interaction  ...
  5. Antibodies 101: Conjugation

    Type
    Blog Post
    ... but it has a strong affinity for avidin and streptavidin, both of which can bind up to four biotin molecules...
  6. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...this proof-of-concept study) are captured on streptavidin-coated magnetic beads through an assembly of...
Showing: 1 - 20 of 20 results