Antibodies 101: Beyond Surface Labeling
Type
Blog Post
...fluorophore-conjugated streptavidin protein, which binds the primary antibody through the biotin-streptavidin interaction...detection. The advantage of this system is that streptavidin conjugates are commercially available for a ...fluorophore selection for panel design.
Find streptavidin plasmids and biotin plasmids at Addgene!
... two-protein labeling by utilizing(a) biotin-streptavidin binding and (b) antibody host species interactions...or protein-protein interactions, like biotin-streptavidin binding and antibody host species reactivity...