...fluorophore-conjugated streptavidin protein, which binds the primary antibody through the biotin-streptavidin interaction...detection. The advantage of this system is that streptavidin conjugates are commercially available for a ...fluorophore selection for panel design. Find streptavidin plasmids and biotin plasmids at Addgene!   ... two-protein labeling by utilizing(a) biotin-streptavidin binding and (b) antibody host species interactions...or protein-protein interactions, like biotin-streptavidin binding and antibody host species reactivity...

... are tagged with streptavidin, the lateral flow strip first captures the streptavidin portion of the probe...biotinylated primers. These are then immobilized with streptavidin coated beads. Fluorescently labelled dCas9 complexes... concept to lateral flow strips that contain streptavidin which can bind to the biotinylated targets. ...

...the glycoprotein, avidin or a similar protein, streptavidin. This strong interaction allows scientists to...hours biotinylated proteins can be captured on streptavidin affinity beads or matrices. The bait protein...Biotin tagged proteins can then be purified using streptavidin and separated via flow cytometry.  Find plasmids...

...egg-white protein avidin (or its derivatives streptavidin and neutravidin) is for all practical purposes...purification of biotinylated protein on blots with Streptavidin-phosphatase (Jackson ImmunoResearch, 016-050...

...mammalian cells with biotin ligase and monovalent streptavidin.” Nature Protocols 3.3 (2008): 534–545. PubMed...Biotinylation Guidelines Expedeon: The Biotin Streptavidin Interaction  ...

... but it has a strong affinity for avidin and streptavidin, both of which can bind up to four biotin molecules...

...the detection antibody is biotin-conjugated, streptavidin (which has a high affinity for binding biotin...

...little was one minute BASU generated a strong streptavidin signal (~250-fold enrichment) when compared ...

...and labeled protein interactors purified via streptavidin pulldown. Following trypsin digest, PPIs are...

...into pieces small enough to be pulled down via streptavidin IP. Biotinylated dCas9 goes along for the ride...

...dCas9, with bound DNA, can then be purified by streptavidin high-affinity purification. This system is both...

...), the synthetic peptide FLAG, the synthetic streptavidin-binding strep-tag and, more recently, OLLAS ...

...direct in situ breaks labeling, enrichment on streptavidin and next-generation sequencing). Using this ...

...this proof-of-concept study) are captured on streptavidin-coated magnetic beads through an assembly of...

...then label that with fluorescently-conjugated streptavidin to get more fluorophores per antibody, or conjugate...

...direct in situ breaks labeling, enrichment on streptavidin and next-generation sequencing (BLESS) (Crosetto...

...fused to a streptavidin core that are able to retain reporter proteins fused to the streptavidin-binding ...