...non-viral) reporter gene (e.g. firefly luciferase or eGFP) flanked by the EBOV-specific gene start and gene... genes in this plasmid would be more difficult compared to if each gene were encoded in separate (and ...performed in the absence of some viral proteins (compared to viral infection). Therefore, the roles/functions...PMCID: PMC3586226. 4. Mühlberger, Elke, et al. "Comparison of the transcription and replication strategies...

...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence...nuclease pair for every genomic target. Due to its comparative simplicity and adaptability, CRISPR rapidly became...it meets two conditions: The sequence is unique compared to the rest of the genome The target is present...throughout the genome, called off-targets, that can impact your experiment. There are many online tools available...effects Sniper-Cas9 - less off-target activity; compatible with truncated gRNAs to increase specificity ... offer high efficiency and low indel formation compared to HDR. Prime editing requires more optimization...) includes tnsA , tnsB , tnsC , and tniQ . In comparison, type V-K includes just tnsB , tnsC , and tniQ...

... complex secondary structure. Today, multiple companies offer long single-stranded DNA production services...maximum Easi-CRISPR efficiency. Easi-CRISPR is compatible with standard microinjection and electroporation...commonly used cassettes, such as fluorescent proteins (EGFP, mCherry etc.), recombinases (Cre, Flp etc.), and...placed to mediate electroporation. Ohtsuka et al. compared traditional microinjection and i-GONAD knock-in...efficiency using a small ssDNA, and they found comparable efficiencies (52 vs 49%). In contrast, Easi-CRISPR...

...derived from AAV9, their efficacy is generally compared to the AAV9 serotype or an engineered precursor...to be administered concurrently to minimize the impact of neutralizing antibodies developed in response...doesn’t lead to enhanced transgene expression, compared to AAV9, in BALB/cJ mice but is more effective... by the Gradinaru lab (Deverman et al., 2016). Compared to AAV9, it effectively transduces neurons and...toward astrocytes, but its neuron transduction encompasses more molecularly-defined neuronal subtypes and...astrocytes and neurons, with greatly increased efficacy compared to AAV9 (Hanlon et al., 2019). This capsid was...relatively even distribution of target gene expression (EGFP, in the depositing manuscript) across the brain....