...pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP with the transgene of interest...vectors that contain IRES-GFP pShuttle Class of transfer vectors that do not contain GFP Additional Vocabulary...throughout virus production. During experiments, GFP can be used to sort cells infected with adenovirus...pAdEasy-1 packaging plasmid 911E4 cells Cell line compatible with the pAdEasy-2 packaging plasmid; Supplies...

...typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive cells, or selection...transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify and enrich positive...repair template and displays reduced efficiency compared to NHEJ knockout. Base editors can make a limited...our AAV Guide ) ∼4.5 kb packaging limit (only compatible with smaller Cas enzymes) Transient or stable...Cas9 and gRNA Choose a delivery method that is compatible with your experimental system. CRISPR efficiency...

...another marker, such as GFP. A researcher can use FACS to sort cells expressing GFP and later expand these...restriction sites but compatible with the Gateway® cloning system? Gateway® compatible vectors use recombination...generation or 3rd generation packaging system. For a comparison of the key differences between the 2nd and 3rd...lentiviral preference for transcribed genes. Studies comparing the lentivirus HIV and the retrovirus MMLV indicate...using this site include subcloning or appending compatible restriction sites onto the insert of interest...until the gene of interest is flanked by sites compatible with those in the vector into which one ultimately...

...plasmids allow cloning of protein coding sequences with GFP-LOVpep, cpPDZ, ePDZb and ePDZb1 as tags. pDawn/pDusk...H134 Widely used variant with larger photocurrent compared to CrChR2 450 ChETA E123T mutation; creates faster...applying optogenetic tools derived from direct comparative analysis of microbial opsins. Nat Methods. 9(...

...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence...nuclease pair for every genomic target. Due to its comparative simplicity and adaptability, CRISPR rapidly became...it meets two conditions: The sequence is unique compared to the rest of the genome The target is present...throughout the genome, called off-targets, that can impact your experiment. There are many online tools available...effects Sniper-Cas9 - less off-target activity; compatible with truncated gRNAs to increase specificity ... offer high efficiency and low indel formation compared to HDR. Prime editing requires more optimization...) includes tnsA , tnsB , tnsC , and tniQ . In comparison, type V-K includes just tnsB , tnsC , and tniQ...