... lead to a wealth of additional experiments investigating the role of individual genes or entire pathways...a population of mutant cells to identify genes involved in a particular phenotype. CRISPR can be readily...specific classes of human genes. What are the steps involved in a CRISPR screen? Performing a forward genetic...

...discovery: Unless your academic lab is closely involved with clinical trials or has ties to industry, ...work. The number of steps, decisions and people involved is staggering. The process is complicated. If ...important to you, make sure that your project will not involve proprietary compounds or information. Where publication...

... this interrogation process, they will almost invariably say something like “Oh,…I didn’t quite appreciate...educators introduce their students to this otherwise invisible molecular world. For the students, the models ...creating physical models of proteins that are being investigated in their labs. Soon after we began working with...

...which are known to be a part of an organelle or involved in intracellular transport pathways, it is possible...direct molecular interaction without further investigation. One fluorescent microscopy method commonly ...useful for your next localization experiment and invite you to make any fluorescent protein constructs ...

...Gräslund lab. Recent findings suggest that aaRSs are involved in inflammation and autoimmune diseases, so these...within a multi-tRNA synthetase complex, the team investigated whether the generated antibodies could bind ...INTRSECT viral preps available! We are expanding our inventory of ready to use viral preps from the Deisseroth...

...Pelham’s first published peptide tagging experiment involved a peptide from the neuropeptide substance P. This...suitable pair for protein tagging. In two studies investigating mechanisms of endoplasmic reticulum localization...versions of Myc1-9E10 mentioned above basically involve taking the Myc1-9E10 variable regions and sticking...

...by meeting with professors - a/k/a Principle Investigators (PIs) - whose labs does research that aligns... receiving applications, universities typically invite a select group of students to continue the application...accessible, inclusive, and accommodating for everyone involved in the process. COVID-19 has changed many aspects...

... you understand the perspective of all persons involved and be used as a guide to point you toward a creative...third example shows, the ultimate resolution can involve ideas not related to the actual data collection... How can I negotiate to show the company that investing in me is also beneficial to them? I have an idea...

... don't have good ways of converting typically invisible information into actionable signals. Let's say...sensors. Those sensors could transduce otherwise invisible molecules into regulation of gene expression including...bioeconomy, and that it’s been 40 years since the invention of genetic engineering. What's incredible is the...

...process. The majority of current cloning techniques involve the assembly of a circular plasmid in vitro, before...Laboratory of Molecular Biology. He is currently investigating the molecular biology of synaptic transmission...and plasticity.     Javier García-Nafría is an investigator scientist at the MRC Laboratory of Molecular...

...the polyA sequence is inverted only in the Cre-expressing tissues. This inversion creates a template that...Yoo BB, et al. Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous...

...protein involved in neurodegeneration in Parkinson’s disease. Knowing the primary investigator where the...available for distribution. If your experiment involves delivering a calcium biosensor using AAV, you ...

...want the works: organizing reagent storage and inventory, managing orders, tracking students and research...access your protocols at the bench, keep your inventory at your finger tips, and make shopping lists as...protcols, sign-up for shared equipment, and track inventory and orders. Quartzy caters to life science labs...

...scientist. I recently was invited to attend the first Principal Investigator meeting of the NSF EDGE (... students. You can see why I was excited to be invited to attend. My job is to ensure that, in general...

...of collecting fibroblasts, which require a more invasive skin biopsy procedure. This becomes a major benefit...grade hiPSCs, reprogramming methods that do not involve modification of the original genome by integrating...cell. The method also reduces biosafety concerns involved in production and usage of Sendai virus. Reprogramming...

... This will give you a feeling of what might be involved in such a role. In addition, it can be advantageous...extent there is the need (or opportunity) to get involved in product testing in the lab, to travel to customers...outreach, and more. Technical support roles also involve interacting with other departments such as research...

...Scrum is that it provides transparency to everyone involved about the status of each project. This creates...example, we recently reduced the number of steps involved during checkout. Demo details for this would be...writing code for common tasks, it would be like re-inventing the wheel over and over again for every single...

...cell lines can become cross contaminated and are invariably much older than those available from biorepositories... NDRG2 Expression Attenuates Proliferation and Invasion in Renal Cancer Cells, Marker-independent identification...Thermofisher Guidelines for Maintaining Cultured Cells Invitrogen Cell Culture Basics Guide Abcam Cell Culture ...

... genes mutated in human cancers have a homolog involved in yeast cell division (Pray, 2008). Scientific...common protein found in lewy bodies), have been invaluable in understanding the basic molecular mechanisms...other model organisms. Worms have been used to investigate an array of biological systems and processes ...

...2013). Since then, Spinach2 has been used to investigate intracellular viral DNA dynamics in neuronal ...developed into a split aptamer that can be used to investigate RNA assembly. The split aptamer is composed of... blog Learn more about aptamer fluorophores Investigate other fluorescent biosensors Find other uses ...