Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 161 - 167 of 167 results
  1. Church Lab CRISPR Plasmids

    Type
    Collection
    ...nuclease are human codon optimized but function well in E. coli . Bacteria expressing NM and TD may grow slightly...with custom guide RNA (gRNA) in human cells: this involves co- expression of a Cas9 protein bearing a C terminus...
  2. Promoters

    Type
    Guide
    ...operon trp Repressible by tryptophan Promoter from E. coli tryptophan operon Ptac Regulated like the lac...operator preventing transcription. The trp operon involved in the production of tryptophan is an example ...
  3. Zhang Lab CRISPR Page

    Type
    Collection
    ...screening in human cells. Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelsen TS, Heckl D, Ebert BL,...) is a microbial nuclease system involved in defense against invading phages and plasmids. CRISPR loci...increase targeting efficiency. These plasmids use the inverted terminal repeats (ITR) from AAV serotype 2. SaCas9...
  4. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Osmolarity SED1 osmolarity biosensor, for expression in E. coli , yeast, or plants Intrinsically disordered ...this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource... experimental design, biosensors can enable investigation of a signaling pathway or measurement of a biomolecule...2011;6(12):e28245. Uwe Sauer Citrate Direct or inverse fluorescent biosensor for citrate (Citron/Citroff...cytosolic High-Performance Intensiometric Direct- and Inverse-Response Genetically Encoded Biosensors for Citrate...
  5. Optogenetics Guide

    Type
    Guide
    ...Optogenetics Resource Center Boyden ES, Zhang F, Bamberg E, Nagel G, Deisseroth K. 2005. Millisecond-timescale...brain, rendering the experimental procedure less invasive. Different activation wavelengths also make it...Henninger MA, Kodandaramaiah SB, et al. 2014. Noninvasive optical inhibition with a red-shifted microbial...
  6. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Anti-MBP [N128A/4R] Maltose binding protein (MBP) E. coli Mouse IgG2a 206532 Anti-Keyhole limpet hemocyanin...IgG2a 206546 Anti-Thioredoxin [N245/44R] Thioredoxin E. coli Mouse IgG2a 206547 Anti-DNAH7 [N250/21R] DNAH7...separate elements of the R-mAb expression plasmid involved in coexpression of light (green) and heavy (blue...
  7. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource...several sources (Chan Zuckerberg Initiative, MedGen, ClinVar) and is meant to be representative but not exhaustive...
Showing: 161 - 167 of 167 results