... current research. Understanding the depth and breadth of this bias, and then using that understanding...cancer cell lines (left). Heatmap indicating the breakdown of ancestry associated dependencies across ancestry...

... are likely to order several plasmids at once, breaking the statistical independence assumption of the...log-normal likelihoods. Effects of weekends, winter break, and COVID-19 on plasmid requests Figure 5...

...good ideas in biological imaging, the initial breakthrough for in-cell labeling techniques was provided... try out the JF dyes. Acknowledgements I thank Brett Mensh, a wordsmith soldier of fortune, for helpful...

...restaurant chains including Chipotle and Panera Bread have pledged to eliminate the use of antibiotics... machinery, so a CRISPR-induced double-stranded break (DSB) in the genome is lethal. If this DSB occurs...

...cool with your legal department) right in your breakroom. 5. Throw some physical activity into the mix ...happening! Addgene actually has chimes outside of our break room. A ring of the chimes means something fun is...

...single-stranded nicks instead of the double-stranded breaks used in other insertion systems. Off-target nicks...nicks are much less harmful than off-target breaks, almost always resolved without any toxic effects to...

...centrifugation between washes which can cause loss of beads, break weak protein interactions, and limit high throughput...buffer has an optimal pH and ionic strength that breaks weak interactions from residual non-specific proteins...

...group used an approach called BLESS (direct in situ breaks labeling, enrichment on streptavidin and next-generation...overcome with split AAVs. In general split AAVs break a large transgene in two pieces and package each...

...on studies in many disciplines has been a long brewing frustration for me. I read a variety of psychology... missing out on. Yet I believe that the big breakthroughs in my discipline, education, will come from ...

...variety of topics. Individual forums are hosted on subreddits (r/”topic”) which are essentially lists of forums...engaged, and has great questions! 2. The r/Science subreddit is well moderated. One of our original worries...

... healthcare consultant and what you could do to break into the field. What is healthcare consulting? ...from taking up such complex challenges, quickly breaking them up into smaller achievable tasks, and then...

...gRNA Figure 1: Validation of double strand break (DSB) mediated homology dependent repair by enhanced...better that gRNA is at causing a double stranded break at the target site, allowing the two halves of EGFP...

...offense to BRET, CRET and other RET techniques which have never outclassed the Master. Note: BRET= Bioluminescence...

...bases, adenine, thymine, cytosine, and guanine (abbreviated to A, T, C, and G respectively) that are organized...process of molecular cloning enabled scientists to break chromosomes down to study their genes, marking the...series covers topics ranging from the newest breakthroughs in plasmid technologies and research, to overviews...

...frameshift mutations through aberrant double strand break repair, it has proven more challenging to develop...editing via the introduction of a double strand break by using a targeted nuclease. Asuri et al, for example...

...fit a big gene into a tiny vector like AAV? By breaking the transgene into smaller pieces. Reassembly ...this approach is dependent on double-strand DNA break repair, if non-homologous end joining (NHEJ) disrupts...

... (HDR) of a CRISPR/Cas9 induced double-stranded break. This site-directed genome editing requires a template...researcher at Cornell University.       References 1. Breslauer, Kenneth J., et al. "Predicting DNA duplex stability...

...to specific loci without inducing double strand breaks. Below you'll find some of the CRISPR-based epigenetic...programmable and targeted de novo DNA methylation in breast cancer. Oncogene 34:5427–5435 . https://doi.org...

...Part 1: Genetically Engineered Mice and Part 2: Breeding and Crossing Mice. My first CRISPR mouse experiment...endonuclease that creates the DNA double-strand break (DSB) at the target site, and a donor oligonucleotide...

...standard DNA plasmid transfection, a double-stranded break may occur in your cargo. Additionally, with piggyBac...as CRISPR/Cas9) to create a double-stranded DNA break. After DNA cleavage, the piggyBac™ donor is used...