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Showing: 141 - 160 of 320 results

  1. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ... recombinant antibody resource called NABOR (Neuroscience AntiBody Open Resource). The antibodies distributed...of monoclonal antibodies publicly available. The Neuro Mab Sequencing Initiative uses high-throughput DNA...

  2. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...stably integrate a reporter gene that contains a puromycin resistance gene followed by the target site (the...genome edit:  A donor plasmid that contains a puromycin resistance gene linked to an in-frame H2B-GFP....

  4. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...international, collaborative project—funded by the European Commission, the US National Science Foundation...increasingly, corporations in the US and Western Europe have taken three strategic approaches to gender...

  5. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...cell types including endothelial cell-pericyte, neuron-astrocyte, and cancer-stromal cell pairs.   ...new additions.  Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f, now available as a ready-to-use...

  6. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...for Gordon Fishell Lab constructs targeting GABA neurons. Serotypes include AAV1, and AAV9. Find viral ...channelrhodopsin (C1V1) targeting parvalbumin cortical interneurons is now available as a ready to use viral prep...

  8. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ... form of blindness known as Leber’s congenital amurosis (LCA). This disease is caused by mutations in ...central and peripheral nervous systems." Nature Neuroscience 20.8 (2017): 1172-1179. PubMed PMID: 28671695...

  10. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ... morphology, and circuit mapping of a targeted neuronal population.    Figure 2 (modified from...activated and inactivated transgene expression in neurons.  PubMed PMID: 2286602. PubMed Central PMCID: PMC3406316...

  11. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...selectively in Parvalbumin containing cortical interneurons. We now provide ready-to-use AAV vectors expressing...this promoter. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f are now available as a...

  12. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs have enabled discovery...YFP, Strep, TEV-V5) with either blasticidin or puromycin selection. Researchers at the Allen Institute ...

  13. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...and endocrine cells such as AtT-20, PC12, Rin5F, Neuro2A and GH4C1, among others (see Lindberg papers in...of Medicine. Iris Lindberg is a professor of Neurobiology and Anatomy at the University of Maryland Medical...

  14. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...postdoc I’m currently doing an industry postdoc in neuroscience at AstraZeneca’s Waltham site after doing a ... and is applying this towards researching neurodegenerative diseases.   Additional resources on the Addgene...

  15. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...placing FP timer DsRed-E5 under the control of Neurog3 (a gene controlling pancreatic differentiation)...preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric...

  16. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...cells where recombination has occurred. Neomycin, puromycin, and hygromycin resistance genes are commonly ...GFP, as seen in blue flame plasmid OCT4-eGFP-PGK-Puro from the Jaenisch lab.   Figure 3: A knockout...

  17. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...Jake Watson is a postdoctoral researcher in neurophysiology at the MRC Laboratory of Molecular Biology....Laboratory of Molecular Biology. He is interested in neuronal signal transduction, and uses electron cryo-microscopy...

  18. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...pseudoreplication in neuroscientific studies: is it affecting your analysis? Lazic SE. BMC Neurosci. Jan 14;11:...

  19. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...calmodulin-dependent protein kinase II promoter Specific Used for neuronal/CNS expression. Modulated by calcium and calmodulin...expression than U6. May have better expression in neuronal cells. U6 small RNA expression shRNA From the...

  20. The Challenges of Cell Culture

    Type
    Blog Post
    ...Outreach Scientist at LGC Group, London, which is the European distributor for ATCC. He studied biochemistry ... high level of experience he now travels around Europe giving talks and seminars on cell culture and other...
Showing: 141 - 160 of 320 results