CRISPR Guide
Type
Guide
...marker like GFP, researchers have turned dCas9 into a customizable DNA labeler compatible with fluorescence...typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive cells, or selection...transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify and enrich positive...detecting multiple genomic loci, and compatible with live cell imaging. Compared to techniques like fluorescence...nuclease pair for every genomic target. Due to its comparative simplicity and adaptability, CRISPR has rapidly...it meets two conditions: The sequence is unique compared to the rest of the genome. The target is present...gRNA targeting sequence is for the genomic target compared to the rest of the genome. Ideally, a gRNA targeting...