Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
Type
Blog Post
...labeling. Size is also a factor. Bulkier tags, such as GFP, may interfere with protein expression or function...realizing it. Yet, this selection is crucial as it will impact downstream outcomes of an experiment, including...increased versatility in probe attachment, and compatibility with far-red and near-infrared fluorophores....Grimm & Lavis, 2022). These variations directly impact the way you can utilize these dyes. The most-used...may prove challenging to stain intracellular compartments in in vivo model systems, so extra care must...emission of labeled agonists and antagonists, impacting the interpretation of experimental results. It's...dopamine D2/D3 receptors (Allikalt et al., 2020)
Impact of labeling process: The process of dye labeling...