We narrowed to 321 results for: cloning

...developed and implemented by applying the Golden Gate cloning method to human proteins known as Pumilio/fem-3...

...purchasing competent cells. However, for most everyday cloning, homemade competent cells will work just fine.  ...

...around 20 minutes, they are perfect for molecular cloning and metabolic engineering.  The E. coli genome ...out the GreenGate Toolkit which allows for easy cloning of constructs specifically designed for use in ...

...) (Latynski and Valentovich 2014). We routinely clone the purified amplicons upstream and downstream of...transcriptional readthrough into the MCS to facilitate the cloning of toxic products. To allow manipulation of naturally... they are just spontaneous antibiotic resistant clones. But if they are blue or magenta, they are real...

...expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids -  If you're familiar...

...Monoclonal antibodies contain a single antibody clone and therefore a single isotype while polyclonal ...advantages. Since monoclonal antibodies contain a single clone they have higher lot to lot specificity and tend...Since polyclonal antibodies contain a variety of clones and thus the potential to bind many different sites...

...to exons 1 and 3 of the targeted gene have been cloned into the vector on either side of an antibiotic...For this purpose, the sequence to be inserted is cloned into the vector between the homologous sequences...the one shown below, where the sequence of GFP is cloned together with the Neomycin resistance (NeoR) gene...

...Pathway Clone Collection 2.0. The collection is a set of vectors for use with the Gateway Cloning Platform...

...website to verify plasmids or validating your new clones, try these tips to make the most of your time and... to help other labs speed up their plasmid and cloning verification steps and free up more time for using...

...protocol. Or maybe you can’t find the molecular cloning guide you were sure was in one of those four white...

...two vectors are part of the larger pMVP/pMAGIC cloning system, which contains Cas9 fusions for several...Arlotto ME, et al (2018) Creation of versatile cloning platforms for transgene expression and dCas9-based...

...researchers to insert targeting oligos with minimal cloning required. As with other model systems, multiple...containing vectors ready for Golden Gate mediated cloning of your gRNA of interest. Figure 1: Simplified...

...Formosa has deposited six plasmids with a multiple cloning site in place of the epitope tag for creation of...

...specific genomic locus, which can require multiple cloning steps (Fujita et al., 2013). CRISPR-mediated purification...can be targeted using a gRNA, which can easily be cloned into a gRNA expression plasmid. Experimental use...

...easy creation of long hpRNA with Gateway-based cloning and straightforward positive selection of plants...assembly of intron-spliced hpRNAs through Golden Gate cloning. Artificial micro RNA and short hairpin RNA The...

...assembled with other elements using golden gate cloning. This toolkit is designed to be used across many...

...thanks to their small size of 12–15 kDa Ease of cloning and expression from plasmids, due to only needing...

... passing. We’ve recently piloted Addgene’s new cloning grade DNA service, and viral vector distribution...

...ITRs does not get packaged in the AAV vector. The cloning capacity between the ITRs is ~4.4 kb. ITRs are ...

...resources on the Addgene blog Learn about Gateway cloning Read about genome engineering in flies Learn more...