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We narrowed to 333 results for: cloning

Showing: 141 - 160 of 333 results

  3. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...of E. coli for DNA propagation. While great for cloning purposes, these E. coli strains are not usually... (DE3)** Rifampicin RecA-deficient; allows for cloning and expression in same strain F- recA1 hsdR(rK12...

  4. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...directly transfect cloning grade DNA into cells? Cloning grade DNA is designed for use in cloning applications... analyses are performed on my cloning grade DNA order? For each cloning grade DNA prep, the plasmid sequence...like to see available as cloning grade DNA, please let us know using the Cloning Grade DNA Suggestion Form... ordering cloning grade DNA for over 200 of our most popular plasmids! DNA... DNA Service: Cloning Grade DNA ...Details Plasmids Available FAQ Addgene is offering Cloning Grade DNA (cgDNA) for over 200 plasmids in our ...variety of popular plasmids and backbones with high cloning potential. By making these plasmids available as...

  9. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ... al., 2017). Our vectors have two Bsa1 sites to clone the single guide RNA (protospacer+scaffold) in a...single polycistronic tRNA-gRNA (PTG) gene system to clone multiple protospacers which enables us to target...

  10. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...developed for another decade when in 1988 they were cloned by two separate labs (Bird et al., 1988; Huston...an scFv?  scFv sequences have traditionally been cloned by amplifying the VLand VH antibody sequences of...

  11. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...around 20 minutes, they are perfect for molecular cloning and metabolic engineering.  The E. coli genome ...out the GreenGate Toolkit which allows for easy cloning of constructs specifically designed for use in ...

  14. Synthetic Biology - Cloning and Genomic Tools

    Type
    Collection
    ...related to Cloning and Genomic Tools for library construction, genomic modification, and cloning systems....Biology plasmids for cloning and genomic tools. Plasmid... Plasmid Collections Synthetic Biology Cloning and Genomic Tools...Tools Synthetic Biology: Cloning and Genomic Tools SynBio Resources SynBio Home Browse All SynBio Assembly...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...systems. Jump to Table: Cloning and Genomic Tools Reporters and Tags Library Construction Integration Recombination...Recombination Shuttle Vectors Plasmids for Cloning and Genomic Tools Search the table by keyword or sort by...

  16. CRISPR References and Information

    Type
    Collection
    ...gRNA design and cloning gRNA cloning vector PDF, 118 KB Church gRNA design and cloning for Cas9 orthologs... Cas9 PDF, 66.8 KB Fujii gRNA design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo... Fly: gRNA cloning pAc-sgRNA-Cas9 PDF, 169 KB Marraffini Bacteria: pCas9 new spacer cloning pCas9 PDF,...Lander gRNA cloning into pLX-sgRNA pLX-sgRNA PDF, 129 KB Sontheimer gRNA design and cloning All-in-one ... helps design (10 different prediction scores), clone (primer design), and evaluate gRNAs, as well as ...plasmids PDF, 107 KB Chen and Wente Zebrafish: gRNA cloning, in vitro transcription, injection gRNA core ; ...PDF, 110 KB Goldstein Nematode: gRNA design and cloning Cas9-sgRNA construct PDF, 364 KB Goldstein Nematode...

  17. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ... Protocols Plasmid Cloning by PCR Plasmid Cloning by PCR You may also...Designing Primers for PCR Based Cloning The basic PCR primers for molecular cloning consist of: Leader Sequence... for primer design: Because we are cloning an ORF, we want to clone from the start codon (ATG) to the ...PCR based cloning carries a much higher risk for mutation than restriction enzyme based cloning. DNA replication...Protocols for plasmid cloning by PCR. Protocols...Ligation Bacterial Transformation Summary PCR based cloning is incredibly versatile and allows for nearly any...limitations. Background In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at...

  18. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...researchers to insert targeting oligos with minimal cloning required. As with other model systems, multiple...containing vectors ready for Golden Gate mediated cloning of your gRNA of interest. Figure 1: Simplified...

  19. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...cell lines is to transfect, select, and isolate clones. This procedure is cumbersome and inefficient, ... somewhere between one hundred and one thousand clones to find a cell line with the edit of interest. ...
Showing: 141 - 160 of 333 results