...The ABRC collects, preserves, reproduces, and distributes diverse seed and other stocks of Arabidopsis ...

...preparation from the inventory below. Lentiviruses are distributed as purified viral particles in solution that ...

...most popular virus for gene transfer in clinical trials. These viruses have a relatively high transduction...

...pAAV-CBh-mKate2-IRES-MCS (WT-WT) CBh mKate2 Constitutive 2 Patrick 114469 pAAV-CaMKIIa-mCherry CaMKIIa mCherry Constitutive...

...subunit alpha PREX2 Phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 PRKA PRKAA1 ...

...expressing recombinant phosphoproteins. These attributes make rEcoli XpS ( 192872 ) and pSerOTS-C1* ( ...

...encode a wide range of gene products. The main restrictions on a lentiviral transfer plasmid are size (i.e...

... final product should be translated as a single string of amino acids that preserves the sequence...

...on and off systems. Dox also has good tissue distribution, low toxicity, a known half-life (24 hours),...

... is typically used in neuroscience to control electrical potentials in neurons. See our Optogenetics Plasmids...

... Viral Vector Packaging Service , Addgene is distributing ready-to-use viral preparations in engineered...

... evolved AAV variant effective at targeting subventricular NSCs Schaffer 213968 AAV8R-12 Capsid AAV8R12...

...Nature . 520(7546):186-91. PMID: 25830891 Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts...

...filled depends on cellular factors. Multiple labs tried to identify beneficial factors to include in even...bases. Thus, base editors have an activity window restricted to the stretch of ssDNA created by Cas9 binding...

...There is something akin to a frenzy as the crowd tries to weigh and determine the value of every sweet ...This post was contributed by guest blogger Cameron MacPherson at the Institut Pasteur CRISPR software...falciparum (the parasite that causes malaria). I attribute their success to technical expertise, thoughtful...Cas-OFFinder provides a bare-bones data dump of ALL strings in a genome using any IUPAC encoded pattern at ...biotechnology(2014). PubMed PMID: 24880488. 2. Hsu, Patrick D., et al. "DNA targeting specificity of RNA-guided...

... antibody manufacturer for wash recommendations. Tris-buffered saline (TBS) or phosphate-buffered saline...abundant protein if you’re stripping, since you’ll lose protein with every strip cycle. Troubleshooting the...Increase volume of wash buffer Incomplete stripping (If stripping/reprobing) Check for antibody signaling...signaling after each round of stripping* Use a harsher stripping buffer Too little signal Large proteins...Western blots can be tricky to get right. If you’ve thoughtfully done the technical design of your blot...need such precise measurements, you can use a colorimetric ladder, visible to the naked eye; a fluorescent... choose from.  Transfer Transfers are a little trickier than gels and may require multiple runs to optimize...

...e0124958. LOC8-ing mitochondrial fusion with a new tri-chromatic assay Georg Ramm’s lab at Monash University...cumbersome. To overcome many of the limitations intrinsic to DNA-guided systems such as TAL effectors or...modified to use different antibiotic resistances, restriction enzymes and plasmid backbones. pUCXKT contains...gene-specific forward primer containing the desired restriction site (the MCS has several options) and a pUCX-specific...resistance cassette, a ribosome binding site, SacI restriction site and a linker region. The PCR product is ...BMC Molecular Biology. 2013 August 20th; 14(1). TRICK: A method for visualizing the first round of translation...the protein product. The system, cleverly termed TRICK, for Translation RNA Imaging by Coat protein Knock-off...

...antibody. TRIM21, an E3 ubiquitin ligase, is the basis of TRIM-away. In normal physiology, TRIM21 recruits...endogenous TRIM21 is sufficient for TRIM-Away, and you only need to deliver the target antibody. TRIM-Away ...Plasmids Podcast! TRIM-Away: Targeted endogenous protein degradation Article contributed by Mary Gearing... proteasome function. TRIM-away compared favorably to drug inhibition, with TRIM-away targeting of mTOR.... To assess TRIM21 toxicity, Clift et al. created stable cell lines overexpressing TRIM21, finding that...changes in expression of TRIM21-associated genes, indicating that TRIM21 overexpression does not aberrantly ... methods for TRIM-Away delivery. For small cell populations, co-microinjection of TRIM21 protein and the...

...journey. Figure 2: Annual distribution of items to countries with a distributor. Arrows indicate the year...amazing 111 countries (Figure 1)! But while we were making the materials available to many countries, import...country to country.   Figure 1: Number of countries Addgene distributes to per year, 2005-2023. We identified... we identified countries that would benefit from a third-party “helper”: a distributor who, among other... India, and Mexico. Table 2: Addgene distributors Distributor Partnership Start Date Country Served...in material distribution after we partnered with them (Figure 2). In others, the distributor helped solve...that we could identify the need for distributors in some countries. ‌We’ve developed systems to allow ...

...a sequence recognized by the restriction enzyme MstII. When a restriction digest with MstII and southern...than real-time PCR (4). Restriction mapping and modern sequencing Restriction mapping informs on DNA sequence...different restriction enzyme digests. Incubating a DNA sample of unknown sequence with restriction enzymes...has changed, restriction enzymes are certainly not dead yet! Labs still keep restriction enzymes on hand...This post was contributed by guest blogger, Krissy Lyon, a PhD candidate in Neuroscience at Harvard University...three historical techniques: southern blotting, restriction mapping, and sequencing gels, as well as their...fluorescence for fluorescently labeled probes. Colorimetric and luminescence based detection methods are...