...transcription none, need Cas9 plasmid Joung Do you have suggestions for other plasmids that should be added to this...

... predicted protein-coding genes. Do you have suggestions for other plasmids that should be added to this...

...Plasmid Collection and Resource Center Do you have suggestions for other plasmids that should be added to this...

...Insert Vector Type Promoter Tags PI Do you have suggestions for other plasmids that should be added to this...

...Promoter PI Publication Return to top Do you have suggestions for other plasmids that should be added to this...

...CRISPR Blog Posts The David Bikard Lab at the Institute Pasteur has deposited a collection of CRISPR arrays...

...needed. Add stir bar and stir slowly at 4 °C for 1 h, then keep at 4 °C for 3 h without stirring to allow...controller Hazardous waste container pH meter Stir plate Magnetic stir bar Sonicator Ear protection Vortex Reagents...other than PES. AAV particles stick to many other surfaces, but do not stick to PES. Using a PES filter ... FBS media causes less filter clogging during harvesting. The addition of sorbitol has been shown to increase...PEI powder into 100 mL of deionized water. While stirring, slowly add hydrochloric acid until the solution...deionized water and adjust to a final volume of 1000 mL. Stir at RT until fully dissolved. Pro-Tip This step is... challenging due to the high viscosity of PEG. Stirring under medium heat will promote faster dissolution...

...repository feedback form . If you have further questions or comments you can also contact them at contact...

...Activity Serotype PI 99118 pAAV-CAG-tTA CAG-driven, constitutive Expression of the tet-off transactivator (tTA...

...colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created...

...Plasmid Collection Return to top Do you have suggestions for other plasmids that should be added to this...

...Selectable Marker PI Publication Do you have suggestions for other plasmids that should be added to this...

...O'Connor-Giles, Harrison, Wildonger Do you have suggestions for other plasmids that should be added to this...

... LEU2 none, need Cas9 plasmid Lu Do you have suggestions for other plasmids that should be added to this...

...substrates and the ACP and SFP Synthases. Users may still generate their own fluorescent or non-fluorescent...

...Cre-dependent Flp-dependent Cre and Flp-dependent Constitutive (non-cre dependent) Serotype AAV1 AAV2 AAV5 ...

...Description Gene/Insert PI Return to top Do you have suggestions for other plasmids that should be added to this...

...deionized water in a 1 L bottle and stir on a magnetic stir plate. Stir until all particles have dissolved...platform set to 120 rpm 37 °C bead bath Vortex Stir bar Magnetic stir plate pH meter Reagents HEK293 cells Recombinant...check for the presence of particles still in solution. Continue to stir until all particles have dissolved...ensure that you are in compliance with your institution’s biosafety regulations. Last Update: February...

... 26269 319 Gateway Return to top Do you have suggestions for other plasmids that should be added to this...

...second plasmid ( pgRNA_bacteria ) contains a constitutive sgRNA expression cassette on a ColE1 vector ...