...survival tactics of prokaryotes to deliver the technological biological blockbuster known as the CRISPR (...multiplexibility, and ease of use. In order for this technology to survive the diverse demands of the biotech...various innovative biological and translative technologies. Addgene has empowered researchers to harness... idea or application that involves CRISPR/Cas technology? Let us know.  References Ishino Y, Hideo,...

...the chance to win some of the latest tools and technologies in life sciences research. The rare disease ...countries. “Over $600,000 worth of cutting-edge technologies were awarded to study 31 rare diseases,” said...ll combine induced pluripotent stem cell (iPS) technology and CRISPR-Cas9 constructs from Addgene to gene-edit...those mutations, they now plan to use CRISPR-Cas9 technology to create cell lines and/or mouse models carrying...

...DNA sequences with twin prime editing. Nature Biotechnology, 40(5), 731–740. https://doi.org/10.1038/s41587...deletions using paired prime editing. Nature Biotechnology, 40(2), 218–226. https://doi.org/10.1038/s41587...optimized, paired pegRNAs in plants. Nature Biotechnology, 39(8), 923–927. https://doi.org/10.1038/s41587...pegRNAs improve prime editing efficiency. Nature Biotechnology, 40(3), 402–410. https://doi.org/10.1038/s41587...cleavage using CRISPR-directed integrases. Nature Biotechnology, 41(4), 500–512. https://doi.org/10.1038/s41587...

... Figure 1: An image from AAV Purification by Iodixanol Gradient Ultracentrifugation First, you’ll... on our protocol page for AAV Purification by Iodixanol Gradient Ultracentrifugation. The reader may be...text: "The left panel is a profile photo of an iodixanol gradient after ultracentrifugation. The interfaces...of the 40% layer containing the virus and 60% iodixanol layer, with the bevel pointed up. A syringe at...

...many opportunities to learn about and develop technologies that will have impacts on many different facets...ready to discuss their VR helmets, gait tracking technologies, and brain monitors. Walking around the poster... addition to the researcher using sequencing technologies to study microbiome interactions with Alzheimer's...genes to many different cell types with minimal immunological effects. AAVs are used in experiments targeting...

... the best product-market fit for this set of technologies, effectively communicating the advantages embodied...successful if they are 100X better than current technology and get customers to change behavior. When you...raised and the company may begin to prove the technology. This transition takes around 1 – 2 years. ... The product is the measure of success. Technology is proven in (A) Productize, (B) Roll out to market...

...channelrhodopsin-based optogenetics minimally-invasive, Frances Arnold’s lab and Viviana Gradinaru’s lab used machine learning...design challenges To overcome these challenges, the Arnold and Gradinaru Labs developed a machine-learning...guarantee their success in vivo. Therefore, the Arnold and Gradinaru Labs expressed ChRgers in mice to..., Yang KK, Robinson JE, Mackey ED, Gradinaru V, Arnold FH (2019) Machine learning-guided channelrhodopsin...

...innovations” stimulates excellence in science and technology by integrating sex and gender analysis into research... disease being treated, and other hormonal, immunological, and environmental factors. How to include sex...international expert on gender in science and technology and currently directs the EU/US Gendered Innovations...Nations on the topic of “Gender, Science, and Technology” in 2011 and again in 2014. Resources on the ...

...Voigt: The top products out of biotechnology today are things like butanol - very simple molecules - a few...it, his lab at the Massachusetts Institute of Technology has been “working on new experimental and theoretical... collision that’s taking place between CRISPR technology and synthetic biology, the tools he’s making ...

...recombination. What is Cre-lox? The Cre-lox system is a technology that can be used to induce site-specific recombination... lethality. To facilitate the use of Cre-lox technology, transgenic mice have been constructed that express...system is another site-directed recombination technology very conceptually similar to Cre-lox, with flippase...analogous to Cre and loxP, respectively. The FLP-FRT technology can be an effective alternative to Cre-lox, and...

...revolution for antibodies. Recombinant antibody technology makes it easy for scientists to share, remix,...proposed a shift to recombinant DNA-based antibody technologies (Bradbury & Plückthun, 2015a).   Figure...monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife 8: . https://doi.org/10.7554...srep31730 Tsuruta LR, dos ML, Moro AM (2018) Display Technologies for the Selection of Monoclonal Antibodies for...

... Potential Applications in Biotechnology. Critical Reviews in Biotechnology, 24(4), 155–208. https://doi.org...virulence plasmid are now used routinely in biotechnology to transform plant cells. Degradative plasmids...Nature’s toolbox for new and exciting molecular technologies!  This post was originally written by Jessica...

...Susanna Stroik Even with the advances in CRISPR technology over the past decade, primary cells remain difficult...using peptide-assisted genome editing. Nature Biotechnology, 10.1038/s41587-023-01756-1. https://doi.org...monomeric StayGold fluorescent protein. Nature Biotechnology, 1–4. https://doi.org/10.1038/s41587-023-02018...throughout the non-human primate brain. Nature Nanotechnology, 18, 1241–1251 (2023). https://doi.org/10.1038...

...ideas (how to stop rhino horn poaching using biotechnology) were thrown on the table and dissected with...reputation. The public is can be fearful of this technology and angry at the agriculture companies that produce...opportunity has given us the chance to develop the technology we will need, and the business training to make...and Biochemistry from the Illinois Institute of Technology in 2004, she accepted a commission in the United...

...qPCR Using SYBR Green TechnologyAAV Titration by ddPCRAAV Purification by Iodixanol Gradient UltracentrifugationAAV...Figure 1: Overview of key steps of AAV production, iodixanol gradient purification, and AAV titration by qPCR...purified by density gradient centrifugation (e.g., iodixanol) using a high-speed ultracentrifuge. During the...

...Prime editing is a versatile genome editing technology that allows precise modifications of DNA (replacements...features and DNA repair determinants. Nature Biotechnology 2023, 1–11. https://doi.org/10.1038/s41587-023...and product purity by deep learning. Nature Biotechnology, 41, 1151–1159. https://doi.org/10.1038/s41587...efficiency across diverse chromatin contexts. Nature Biotechnology. https://doi.org/10.1038/s41587-024-02268-2 ...

...must stay current with literature and emerging technologies to remain competitive and relevant. Enter: the...department, we had two recurring journal clubs on Immunology and Microbial Pathogenesis that occurred once...spirited and focused as opposed to a wandering monologue?  Assuming the feedback is generally positive ... Will Arnold...

... last 5 years to incorporate more science and technology into the school curriculum. Our Parent Teacher...and the internet before it, genome engineering technology is moving along much faster than it can be officially...sugarcoat the potential dangers of people using the technology in an unethical manner, but I also tried not ... timely.” Genome engineering, and many other technologies and fields in biology, are moving so quickly...

...Figure 1). Citorik et al. targeted beta-lactam and quinolone resistance genes in E. coli using plasmid and ...plasmid. In the case of the genomically encoded quinolone resistance, mediated by a single base pair change...efficiently-delivered RNA-guided nucleases.” Nature Biotechnol. 32(11): 1141-5. (2014). PubMed: PMID 252409288...produce sequence-specific antimicrobials.” Nature Biotechnol. 32(11): 1146-50. (2014). PubMed: PMID 25282355...

... for clinical diagnostics, genotyping, and biotechnological advancements. Many methods of nucleic acid...Biosciences has been started based on DETECTR technology with the mission to “provide a CRISPR-based platform...strip format. They published this in Nature Biotechnology. Like the SHERLOCK detection system, DETECTR...CRISPR–Cas12-based detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0513-4 Chen...