...when we share? NanoLuc® Luciferase-based tools are fantastic examples of technologies that can accelerate...research materials and information. Many of our technology platforms like HaloTag® Fusion Protein, codon-optimized...codon-optimized Firefly luciferase genes (e.g., luc2), and NanoLuc® Luciferase are available from the repository. ...accelerate your research. The creation of NanoLuc® Luciferase from a rather dull 19kDa Oplophorus luciferase...luciferase by Promega’s Advanced Technologies group as well as evolution of the coelenterzine substrate to the...I’ll cover two great applications of the NLuc technology. The first couples NLuc to fluorescent proteins...fluorescent proteins to make better biosensors. NanoLuc®-fluorescent protein fusions for imaging Fluorescent...

...dramatic spread of the Cas9 technology: "The rapid adoption of the Cas9 technology was also greatly accelerated...you think these technologies are going? What is the next hot molecular biology technology? Have you deposited...unique position to observe and quantify how new technologies are being disseminated through the scientific... plasmid sharing In a recent review on CRISPR technology, for example, the authors Patrick Hsu, Eric Lander...

...toolkit based on the Multisite Gateway® technology from Life Technologies. The MultiSite Gateway Kit is a...continually impressed with the amazing plasmid technologies developed by our community of depositors. With...plasmids 62887 & 62888) Zetsche et al., Nat Biotechnol. 2015 Feb 2;33(2):139-42. doi: 10.1038/nbt.3149... system. The ease and flexibility of the LACE technology makes this system widely accessible for many ...imaging and inducible gene expression Brilliant technologies adopt basic biological concepts and employ them... the Weissman lab have teamed up and advanced technology in the fields of single molecule imaging and ...possibility of dimer formation. Prosser et al., Biotechnol Lett. 2015. 37:383-389. Toolkit for constructing...

...Addgene which allow scientists to use CRISPR-Cas technology to perform genome-wide knockout screens. These... Looking for background information on CRISPR technology? See our improved CRISPR-Cas Guide. Wang et...new tool for peptide-peptide locking. The new technology is known as SpyLigase and is a protein domain...E690-7. For further reading about SpyLigase technology, read the Addgene interview with Mark Howarth... and his team at the Institute of Science and Technology Austria, decided to take a novel approach to ...doi: 10.1038/nmeth.3179. Martell et al., Nat Biotechnol 2012 Nov;30(11):1143-8.  A new tool for CRISPR...20;2(9):537-41. DREADD-based chemogenetic technologies After several years of distributing his...

... distributed plasmid technologies pSV-PGC1 - Ok, it's not actually a technology, but pSV-PGC1 was Addgene's...institutions. This has given us a unique window into technology trends in the life sciences. In this post, we'll...give you an inside look at the Top 10 plasmid technologies distributed through Addgene over our first 10...genetically encoded calcium indicators were the hot technology of the year in our collection. CRISPR/Cas9 - ...Year of the CRISPR." The newest genome editing technology has been used and modified by so many labs and...

...the limited technologies available. Over the past few years, however, remarkable technological advances ...and Jinfang Zhu. "Immunologic applications of conditional gene modification technology in the mouse." Current...revolution in modern neuroscience, an era in which technologies like FLEx and optogenetics meet, allowing scientists...mammalian neurons and alter synaptic events: a new technology was born, and the term “optogenetics” was coined... able to distribute materials containing FLEx technology. Read the genOway press release for more information...Deisseroth, Karl, et al. "Next-generation optical technologies for illuminating genetically targeted brain ...at the cellular level in the mouse." Nature biotechnology 21.5 (2003): 562. PubMed PMID: 12665802. SSharma...

...retroviral enChIP system using CRISPR technology. Can you describe this technology and some of its potential applications...development of new biological technologies. The locus-specific ChIP technologies consisting of iChIP and enChIP... to develop new technologies. Majority of them are eager to just "import" technologies developed abroad...The Fujii lab specializes in developing novel technologies to analyze molecular mechanisms of genome functions...extensively with enChIP systems. Can you describe this technology and its advantages? Fujii: In the last several...years, my lab has been working on development of technologies for biochemical analysis of genome functions... chromatin immunoprecipitation (ChIP) (iChIP) technology, which uses locus-tagging and affinity purification...

... AAV purification with iodixanol gradient ultrafugation Overview: Iodixanol gradient ultracentrifugation...brief overview of how iodixanol purifications works, shows how to create the iodixanol gradient layers, and...master iodixanol AAV purification. If you’re new to creating gradients, practice making the iodixanol layers...feel for layering the iodixanol solutions and let me double-check that the iodixanol gradient solutions ...and freeze/thaw the lystate four times in a 95% ethanol and dry ice bath to release the virus particles...then treated with benzonase immediately before iodixanol purification. This approach gives me a bit of ...uses a gradient of different concentrations of iodixanol to separate out contaminants from an impure AAV...

...transformation technologies like those pioneered by Ewen Mullins of Ireland’s Teagasc.  Plant biotechnology is full...there are a lot of unexplored areas in plant biotechnology that are ripe with opportunity. We’ve decided...One of the biggest difficulties that plant biotechnologists face is public perception of genetic modification... crops has burdened this emerging engineering technology. Monoculture and chemical spraying, mainstays...point that people cannot imagine any use for GM technology other than to sustain those techniques. We feel... current benefits and future potential of the technology to the public in an open, responsible manner....this challenge directly by developing a plant biotechnology product specifically for consumer sales. Flowers...

...that had Platforms with a capital ‘P’ focused on technology ... has been critical to the Broad’s success ...biological questions, you need a tremendous amount of technology development alongside it.” Doench starting working...Perturbation Platform and encompasses a variety of technologies to functionally investigate the human genome...genome. Doench says he enjoys working on technology development under the Platform model at the Broad Institute...Institute because they are “not just developing technology for its own sake,” but work in true collaboration...history of RNAi left him a bit skeptical of the technology at the start of the CRISPR craze, but he says...justified… it has been just an incredibly useful technology.” He goes on to explain that, “everything that...

...recombinant DNA technologies that are now commonplace in laboratories. As scientific technologies rapidly progress...computers, cell phones, and cars become more technologically advanced leaving earlier versions obsolete,...perusing classic papers or are brainstorming new technological innovations. Let’s take a look at three historical... use of Sanger sequencing. Building upon this technology, radiolabelled nucleotides were replaced with... DNA sequencing machines (9). Today, similar technologies, sometimes referred to as sequence DNA as it...properties of nucleotides (8). Compared to older technologies nanopore-based DNA sequencing machines are relatively... is projected that nanopore-based sequencing technologies could one day sequence an entire genome for ...

...Scientist at the Broad Institute, John Doench. CRISPR technology has made it easier than ever both to engineer...is true when designing gRNAs for using CRISPR technology – the “best” gRNA depends an awful lot on what...Gene knockout by NHEJ Gene knockout with CRISPR technology is usually accomplished by Cas9-mediated dsDNA... Created with BioRender.com. Two newer technologies offer an alternative to HDR for introducing ... another target C or A in the window. Another technology, prime editing (reviewed in Anzalone et al., ... although these are still early days for this technology, and the user may need to optimize numerous parameters...CRISPRa (activation) and CRISPRi (inhibition) technologies – a nuclease-dead Cas9 (dCas9) is directed near...

...please read on to learn more about this exciting technology from the folks at Transposagen. This post was...engineering approaches. Most importantly for this technology, however, is that the transposase also enables... with no known upper limit [1]. The piggyBac™ technology can be used for numerous applications, including...of interest. Fortunately, by combing nuclease technology with the piggyBac™ system, these challenges can... DNA using the piggyBac™ transposon selection technology using a site-specific nuclease to stimulate homologous...of using piggyBac™, you could use recombinase technology (Cre/loxP) to remove the donor cassette. While...summary, the piggyBac™ transposon is a powerful technology that may be used for a variety of research applications...

...acid-guanidinium-phenol solution that combines the homogenization solution and phenol addition of the ...extraction protocol with guanidinium thiocyanate-phenol-chloroform. In this method, samples are still homogenized...using cesium-chloride gradients, water-saturated phenol, sodium acetate, and chloroform are added to the...centrifugation (not ultracentrifugation!), the layers of phenol and chloroform separate, and RNA is retained in...aqueous layer is extracted and RNA can then be isopropanol precipitated. This method reduced the time it...laboratory situation or tissue collection method, so biotechnology companies have marketed several products that...of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction. Analytical Biochemistry ...

...simple technology,” Doudna further explained. “That’s why we’re seeing adoption of the technology in different...University.  There can be no doubt that CRISPR/Cas9 technology has been a breakthrough for the genome-editing...CRISPR for complex genetic diseases As CRISPR technology becomes increasingly more cost effective and ...of those off-target mutations when using this technology. When CRISPR therapies are being given in vivo... “A glance at genome editing with CRISPR-Cas9 technology.” Current genetics. https://doi.org/10.1007/s00294... Khatibi et al. 2021.  “CRISPR Genome Editing Technology and its Application in Genetic Diseases: A Review...Review.” Current pharmaceutical biotechnology. https://doi.org/10.2174/1389201021666200621161610 4. Uddin...

...media change into methanol and peroxisomal autophagy can be induced by removing methanol from the media....protein animal feed. Due to increasing prices of methanol and advancements in other biomass production techniques...Preferred carbon source Glucose Glucose Glucose, methanol, glycerol Heterothallic mating Efficient... a methylotropic yeast, meaning it can utilize methanol as its sole source of carbon and energy (as well... its powerful promoter of a gene important for methanol usage. This gene promoter, AOX1, was co-opted ...all eukaryotic cells and peroxisomes are where methanol metabolism occurs in methylotrophic organisms....Riley, R., et al., Comparative genomics of biotechnologically important yeasts. Proc. Natl. Acad. Sci.,...

...learn more about “... recent findings in basic immunology, cancer-immune cell interactions, cancer immunotherapy... conference was that Cancer Researchers and Immunologists are coming together to tackle the challenge ... from the conference which reflect how cancer/immunology researchers are preparing and carrying out this...inflammation and immunosurveillance have a look at our Immunology Research Plasmids and Resources. Studying the...the use of optogenetic tools is expanding to immunological research. Here the Zhou group presented Opto-CRAC...to calcium. We’d love to expand our cancer and immunology resources. If you have a great new plasmid you...Plasmids Browse Immunology Plasmids Find Kits for Cancer Research  ...

... CRISPR-based cytosine base editing. Nature Biotechnology, 41(5), 663–672. https://doi.org/10.1038/s41587...activity and therapeutic application. Nature Biotechnology, 38(7), 892–900. https://doi.org/10.1038/s41587...concurrent adenine and cytosine editing. Nature Biotechnology, 38(7), 861–864. https://doi.org/10.1038/s41587...engineered Cas9-cytidine deaminase fusions. Nature Biotechnology, 35(4), 371–376. https://doi.org/10.1038/nbt...optimization and ancestral reconstruction. Nature Biotechnology, 36(9), 843–846. https://doi.org/10.1038/nbt...editors generated from TadA variants. Nature Biotechnology, 41(5), 686–697. https://doi.org/10.1038/s41587...editor with low off-target activity. Nature Biotechnology, 41(5), 673–685. https://doi.org/10.1038/s41587...

...thanks to these technologies but many questions remain. The use of other technologies such as optogenetics...could also be tracked by cell sorter giving immunologists the ability to count cells and statistically...different immune cells. These methods allowed immunologists to develop a spatiotemporal view of an immune... time. This technique was first Introduced to immunology by three papers in the journal Science in 2002...optogenetics and CRISPR/Cas9 will help immunologists create even better tools and models to further our understanding...infected with Toxoplasma gondii." The Journal of Immunology 182.10 (2009): 6379-6393. PubMed PMID: 19414791...interfollicular region of lymph nodes." Nature immunology 9.2 (2008): 155-165. PubMed PMID: 18193049.  ...