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  2. Science Career Options

    Type
    Blog Post
    ...roles, including: Livestock Breeder Scientific Illustrator Plant Breeder Zoological Worker Conservationist...

  3. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...important biological information, amino acid abbreviations, and structures into one convenient resource...say that I still have the single and triple abbreviations of the amino acids memorized, but the structures...

  4. Components of CRISPR/Cas9

    Type
    Blog Post
    ... Cleave target DNA resulting in a double-strand break (DSB) Cas9 and its variants have two endonuclease... which is E) cleaved leaving a F) double-strand break. A “gRNA” is a researcher-designed hybrid of the...

  5. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...and ideas ranged from a biological breathalyzer to pesticide breakdown, production of fire-resistant housing...

  6. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...elicit downstream signaling cascades.  So let’s break down the name "G-protein coupled receptor" into ...via bioluminescence resonance energy transfer 2 (BRET2). Check out our blog post on GPCR signaling using...

  7. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...principle of bioluminescence resonance energy transfer (BRET). Photons emitted by a Renilla luciferase variant...exhibited 3-5 fold increased brightness over previous BRET probes, with sufficient signal for in vivo and in...

  8. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...unedited site distal tothe predicted double strand break, and a NHEJ/ “drop-off” probe designed to bind directly... directly at the predicted double strand break site (Figure 2A). The single molecule resolution of digital...

  9. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...upregulation is correlated with poor survival in breast cancer patients. By digging into the literature...containing RNAs could play a role in the severity of breast cancer. Infectious Disease. RaPID can also help...

  10. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...untreatable until 2008. Following a single dose of subretinal administration of rAAV2 carrying the human gene...enhances endonuclease delivery for double-strand break repair." Gene therapy 17.9 (2010): 1175-1180. PubMed...

  13. 6 Tips for Grant Writing

    Type
    Blog Post
    ... else to read and / or give yourself a bit of a break before you read over it again with fresh eyes and...your institute or a private funding body. Success breeds success and I have certainly found that the experience...

  14. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...it before, during, and after the meeting. Let’s break it down into the following: the elevator pitch, ...networking for the day (or whenever you have a small break), write down some notes on the card so you remember...

  16. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...mechanism to accurately repair harmful double stranded breaks, in which nucleotide sequences are exchanged between...CRISPR can target a genomic sequence and create a break that can be repaired by homologous recombination...

  17. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ..., 2018. Most of the time, plasmid prepping is a breeze. You get your stab from Addgene, streak for single...nutrient-rich broth such as Terrific Broth, 2xYT, or a home-brew optimized for plasmid yields. Spiking your media...

  18. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...your colleagues. Top of the plasmid page The ‘breadcrumb’ trail at the top of any plasmid page gives the...nested in. You can see in the example above that the bread crumbs trail starts in the browse page, continues...

  19. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...resistance gene you select? In this blog, we’ll break down the mechanism of action of the most common ...death by intercalating into DNA and generating DNA breaks. The Sh ble gene provides zeocin resistance by ...

  20. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...is used to separate the beads from the media (Brechmann et al. al., 2021) while the media is aspirated....2019.12.017. Epub 2019 Dec 28. PMID: 31891733. Brechmann NA, Schwarz H, Eriksson PO, Eriksson K, Shokri...
Showing: 141 - 160 of 373 results