...! In most academic and nonprofit institutes, the Tech Transfer department is responsible for managing ...deposit a plasmid with Addgene, your organization’s tech transfer office is sent a deposit agreement. The...approach, which we’ve updated and refined as new technology becomes available. Our one-time deposit agreement...agreement means we only have to work with one tech transfer office when materials are requested, instead ...organization upon purchase. We recognize exceptionally fast tech transfer offices with the Golden Stopwatch award...questions about our MTA process, you can check out our Tech Transfer Webpage, help center articles, and our ...information. Happy transferring!    Many thanks to our Tech Transfer team members Maura Coughlin, Darcy Morse...

... and sequence displays are now powered by GSL Biotech's SnapGene Server Software. With the backing of ..., tags, and certain ORFs. Their sophisticated detection algorithm easily and precisely identifies plasmid... Many of these features, such as Cas9, weren’t detected by our in-house software. In fact, SnapGene’s ...includes nearly 3x as many features as could be detected by our in-house software and also eliminates many...within the plasmid. The list of enzymes that can be detected is 5x greater than our previous mapping software...The table found here lists the common features detected in the plasmid sequence. The table includes feature...contains a table listing commonly used primers detected in a given nucleotide sequence. The table includes...

...contributed by Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans Health Administration...roles and my current work as a AAAS Science and Technology Policy Fellow (STPF) at the Veterans Health Administration... come from a wide variety of backgrounds with technical expertise that ranges from astronomy to bioethics...these resources compiled by the AAAS Science and Technology Policy Fellowship Program to learn more about...could understand how to make new treatments and technologies accessible to patients. And what better place...guest blogger Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans Health Administration... Guerra, PhD, is currently a AAAS Science and Technology Policy Fellow at the Veterans Health Administration...

...higher order oligomers can be analyzed by several techniques. Gel filtration and SDS-PAGE under non-denaturing... Whether higher molecular weight complexes are detected will depend on the homodimer affinity and the ...qualitative view of dimerization tendency. Another technique that separates molecules based on size is ultracentrifugation...based on fluorescence polarization) can be used to detect homodimerization. However, all the aforementioned...these assays. However, these strategies will not detect weak dimerization tendency, as is present in EGFP... a cell-based assay that does a better job of detecting homodimerization tendency. This assay employs ...substantial (e.g, the poor performance of mRuby2 is detected by Constantini et al (2015) and by Bindels et ...

...Colorful CRISPR technologies are helping researchers visualize the genome and its organization within...specific loci has historically been difficult, as techniques like fluorescent in situ hybridization (FISH)... locus...unlike CRISPR. Early CRISPR labeling techniques allowed researchers to visualize nearly any single...and movement of this locus in live cells! This technique was developed in the lab of Addgene depositor ... the method is specific and sensitive enough to detect gene copy number based on the number of fluorescent...to mapping pairs of intrachromosomal loci. The technique successfully resolved loci with physical map distances...and engineered sgRNAs using CRISPRainbow.” Nat Biotechnol. 34(5) (2016): 528-30. PubMed PMID: 27088723....

... time working on interesting biotechnology code.There are valid technical reasons to choose a non-Python...particular plasmid and now it’s time for our lab technicians to retrieve the sample and get it ready for shipping...simply follows a linear progression directing our techs through the process. The problem? As we said above...to do when something fails. While we’re sure our techs could figure it out if they needed to, they’d have... when a tricky procedure failed and one of your techs started over with a new tube. In the first example...science_name`. You'll need to decide given the technical details of the problem you're solving, but consistency...

...generate a targeted break. These advances in gRNA technology have enhanced targeting efficacy as well as the... as they will be recognized as foreign RNA. To protect the RNA from degradation, several simple methods...added to the 2’ hydroxyl group of the ribose to protect it from nucleolytic attack. Another stabilizing...just one modification alone (Hendel, et al., Nat Biotechnol; Rozners, JACS). These modifications can be done...switches for gRNAs A major drawback of CRISPR-Cas9 technology is that most expression systems are constantly...system can initiate targeted editing events. Both technologies utilize chemical modifications to the RNA to...CRISPR-Cas genome editing in human primary cells. Nat Biotechnol 33, 985–989 (2015). https://doi.org/10.1038/nbt...

... mice had no detectable editing. Additionally, off-target spleen editing was not detected and Nanoblade...efficiency and off-target effects compare to existing technologies, and their use for HDR editing and mouse-model...compared head-to-head with existing CRISPR delivery technologies, Nanoblades had higher rates for editing efficiency... targeting the Fto gene, while editing was not detected in cells electroporated with RNPs using the same...cytoplasm or pronucleus of the zygote which is both technically challenging and can negatively impact zygote ... from Tyr Nanoblade treated zygotes, five had detectable Tyr editing at the phenotypic and genotypic levels...

...attempted to use the technique for epitope tagging but only one of them could detect knock-in of tags at...NgAgo survey Since it was first published in Nat Biotech in May 2016 (1), NgAgo has received much attention...indel formation (gene disruption) with this new technology. To dig more into what researchers felt about...responded to the survey. When asked if they could detect indels (an indication of gene disruption ability...genome editing with NgAgo, this does not mean the technology is dead in the water. On the bright side, successful...Natronobacterium gregoryi Argonaute." Nature biotechnology (2016). PubMed PMID: 27136078. 2. Burgess, Shawn...

... post was contributed by Kevin Esvelt, a Wyss Technology Development Fellow at the Wyss Institute and ...widespread trust in scientists to develop new technologies with care and humility. Nothing would damage...could not be trusted to handle such a powerful technology responsibly. An accidental release would be particularly...Finally, gene drives are an inherently collective technology: because they necessarily alter the shared environment...Thank you to our guest blogger! An inventor of technologies that harness evolution, Kevin Esvelt studies...ensuring that gene drives and other collective technologies are developed safely and transparently.  For...101: a blog series on the basics of CRISPR-Cas technology CRISPR Resources from Addgene   ...

...partners with other techniques! Minimizing false negatives: Interactions leading to detectable reporter signals...expression (Figure 2c). The reporter gene then allows detection of those cells where binding between the proteins...as using five independent protein interaction detection methods (6). In order to bind DNA and activate... Fields, S., Song, O. A novel genetic system to detect protein-protein interactions. Nature. 1989; 340...Y.l. Split luciferase as an optical probe for detecting protein-protein interactions in mammalian cells... D., and Frommer, W.B. K+ channel interactions detected by a genetic system optimized for systematic studies...

...endeavor including but not limited to biotechnology, chemistry, architecture, engineering, mathematics, physical...developments.  Further, this post may contain technical inaccuracies or typographical errors.  No action...non-bench research career such as law, medical/technical writing, clinical, regulatory, product development... sector are transferrable to industry such as technology transfer, communications, medical writing, compliance...your expert skills your letter should include technical information followed by a plain language explanation...Casseus Law. Driven by her own love of science and technology, Rachel is proud to assist companies and foreign...professionals.  We work with organizations in the biotech, medical device, and diagnostics, healthcare IT...

...Thomason et al., 2014). In recent years, CRISPR-Cas9 technology has also aided in the creation of scarless mutations...accomplish the desired genomic modification. This technique is frequently called allelic exchange. Here, a...replaces the chromosomal copy. This is a versatile technique, and is less prone to off-target mutations. Ultimately... exchange vectors Allelic exchange is a great technique for the scarless modification of bacterial genomes...continue to improve the efficiency of this versatile technique. Many thanks to our guest blogger Jacob Lazarus...tightly regulated and tunable promoter system." Biotechniques 40.3 (2006): 355-364. PubMed PMID: 16568824....genomes using CRISPR-Cas systems." Nature biotechnology31.3 (2013): 233. PubMed PMID: 23360965. PubMed...

... editing technologies have been shown to be excellent alternative genome editing technologies especially... traditional plant breeding approaches, these techniques are cumbersome, often requiring several rounds...domains, and methods of delivery - the underlying technique of CRISPR mediated genome engineering in plants... single-walled carbon nanotube carriers. Nat Nanotechnol 14:447–455 . https://doi.org/10.1038/s41565-019...confers heritable resistance to powdery mildew. Nat Biotechnol 32:947–951 . https://doi.org/10.1038/nbt.2969...Applications of CRISPR–Cas in agriculture and plant biotechnology. Nat Rev Mol Cell Biol 21:661–677 . https://...

...out at techtransfer@addgene.org and we will initiate a conversation with you and your technology transfer...limitation, we worked closely with the legal partners and Tech Transfer Offices at universities to make COVID-19... the United States, and many universities and Technology Transfer Offices were, at least, temporarily ...                           California Institute of Technology             Johns Hopkins University                           ...industry scientists, we’ve worked with many scientists, tech transfer offices, research foundations, and funding...

...thinking and problem solving abilities, increased my technical know-how, and helped develop my science communication...scientist.” For jobs away from the bench, look for “technical or product support specialist,” “field application...application scientist,” or “scientific or technical writer,” among others. Don’t discount job postings looking...work in industries you’re interested in (i.e. biotechnology, pharmaceuticals, etc.) Do their jobs sound ...deeper foundation of scientific knowledge and technical know-how that increased my autonomy and satisfaction...from the lab to a desk, although there’s often technical career tracks for PhDs who want to stay at the...

...of the final viral preparation. Through sugar detection assays, in vitro transduction, and silver stain... vectors: Enhancement in vector stability." Biotechnology and bioengineering 94.2 (2006): 322-329. PubMed...applying osmotic pressure and sodium butyrate." Biotechnology and bioengineering 42.5 (1993): 601-610. PubMed... stress." Advances in animal cell biology and technology for bioprocesses (1989): 272-278. 4. Ozturk, ...growth, metabolism, and antibody production." Biotechnology and bioengineering 37.10 (1991): 989-993. PubMed...improves adenovirus production." Journal of biotechnology 157.1 (2012): 228-236. PubMed PMID: 22154564...

...represents a major step forward in facilitating techniques that are dependent on long, error-free, reverse...potential to provide improvements to existing technologies based on polymerase processivity. Please find...allowing for quantitative analysis using different detection methods. Since the anti-GFP nanobody is able to...focuses on proteomics: developing and applying new technologies to quantify proteins in tissues, cells, and ...the group, developed a CRISPR-based approach to detect proteins that associate with specific genomic loci...-APEX2 fusion.  This Sontheimer lab used this technology, which they call C-BERST (dCas9–APEX2 biotinylation...

... Patent Lawyer Technical Writer Meteorologist Journal Editor Textile Technologist/Designer     ...those who are scientifically minded! Listen to Biotech Founder Michael Koeris' Journey from Grad Student...desired, as roles are becoming more reliant on technology, and good computer skills will give you an advantage...range of roles, including: Science Reporter Technical Writer Statistician Bioinformatician Science...with New Careers while in Grad School Listen to Biotech Founder Michael Koeris' Journey from Grad Student...

...developed a paper-based method that can be used to detect microbes and biomarkers on paper. At the heart ... a RNA hairpin that can be designed to bind and detect nearly any RNA sequence. Downstream of the hairpin... Using this system, the Collins lab was able to detect mRNA from 10 bacteria of clinical significance ...comparison to qPCR and demonstrated that it can also detect expression of toxin mRNA from Clostridium difficile...identified with DNA-based qPCR methods, the ability to detect mRNA transcripts of toxins and other transcripts...strategy for bacteria to count cell divisions. The technique, called distributed cell division counting (DCDC...

...rapidly decode the complexity? The CombiGEM-CRISPR technology was developed with the goal of providing an easy-to-use...This blog post will introduce you to this new technology, and guide you through the basics of CombiGEM-CRISPR...particularly interested in developing and applying new technologies to interrogate and understand complex biological... off-target effects of CRISPR-Cas9." Nature biotechnology (2016). PubMed PMID: 26780180. PubMed Central...lentiviral CRISPR-guide RNA library." Nature biotechnology 32.3 (2014): 267-273. PubMed PMID: 24535568....combinatorial genetics in human cells." Nature biotechnology (2015). PubMed PMID: 26280411. PubMed Central...

...opsins or chemogenetic receptors used in these techniques cannot be directly visualized with fluorescence...to ‘tag’ or ‘label’ proteins to facilitate its detection. Fluorescent proteins are most widely used tags... emit endogenous fluorescence that allow easy detection with the appropriate fluorescent illumination ...identified using antibodies and immunohistochemistry techniques. Thus, these proteins provide a versatile method...hM4Di in monkeys. The pre-embedding immungold technique was used to reveal the hM4Di coupled to mCherry...Using a combination of in vivo and anatomical techniques, her research aims to gain a deeper understanding...

...about Mammalian shRNA Tools for RNAi! Cre-Lox technology CAG-GFP-IRES-CRE (Plasmid #48201) This retroviral... and Cre-recombinase. The Cre-lox system is a technology that can be used to induce site-specific recombination...knockout, and selection marker removal. CRISPR technology pdCas9-humanized (Plasmid #44246) Expression ... the pLKO.1 vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408)   This...lentiviral prep from the Addgene viral service. CRISPR technology lentiCRISPR v2 (Plasmid #52961) This is a 3rd...Maetzig T, et al. Gammaretroviral Vectors: Biology, Technology and Application. Viruses. 2011. PubMed PMID: ...

...Interactions Difficult to detect weak and/or transient PPIs. Many PPI identification techniques rely on protein ...version of the proximity-dependent biotinylation technique BioID. The key advantage of Split-BioID is that...proteins. No spatiotemporal control. Many PPI detection methods lack spatiotemporal control or an “on/...extended incubation period could make it difficult to detect proteins with short half-lives. Interested in using...Additional Resources on the Addgene Blog Learn about techniques for visualizing protein turnover Learn about ...

...Many, many techniques are available to assess protein-protein interactions. One popular approach is to... conditions. Shyu YJ, Liu H, Deng X, Hu CD. Biotechniques. 2006 Chang-Deng Hu Green sfGFP Split super-folder...reassembly is a fast, efficient, and robust method for detecting protein-protein interactions in vivo. Blakeley... conditions. Shyu YJ, Liu H, Deng X, Hu CD. Biotechniques. 2006 Chang-Deng Hu Venus Improved N-terminal...high signal-to-noise ratio. Kodama Y, Hu CD. Biotechniques. 2010 Chang-Deng Hu mVenus pET-BiFC contains... labeling for single-molecule imaging TagBiFC technique allows long-term single-molecule tracking of protein-protein...

...revolution for antibodies. Recombinant antibody technology makes it easy for scientists to share, remix,...Antibodies are used by scientists around the world to detect, purify, quantify, deplete, and visualize proteins...antibodies or monoclonal hybridomas, but those techniques have several drawbacks. Polyclonal antibodies...proposed a shift to recombinant DNA-based antibody technologies (Bradbury & Plückthun, 2015a).   Figure...srep31730 Tsuruta LR, dos ML, Moro AM (2018) Display Technologies for the Selection of Monoclonal Antibodies for... for Clinical Use. In: Antibody Engineering. InTech Additional resources on the Addgene blog Read our...

... RNA protectors epegRNAs: epegRNAs include structured motifs at their 3′ end to protect them from...editing is a “search and replace” genome editing technique that relies on a fusion enzyme of Cas9 nickase...editor includes the fused RNA-binding protein La to protect the end of the pegRNA. Adding 3′ polyU tracts to...PE7 (right) offer two different strategies for protecting pegRNAs from degradation. Created with BioRender.com...pegRNAs improve prime editing efficiency. Nature Biotechnology, 40(3), 402–410. https://doi.org/10.1038/s41587...

... Potential Applications in Biotechnology. Critical Reviews in Biotechnology, 24(4), 155–208. https://doi.org...virulence plasmid are now used routinely in biotechnology to transform plant cells. Degradative plasmids...from the benefit of resistance plasmids, which protect the host from attacks by other organisms. To conserve...and others have proposed developing new cloning techniques based on these vectors (Shareck et al., 2004)...Nature’s toolbox for new and exciting molecular technologies!  This post was originally written by Jessica...

...localization of your protein of interest is to make it detectable. Visualization can be achieved using fluorescent...image analysis software are highly sensitive to detecting light signals not visible to the human eye and... imaging such as bleed-through (the incorrect detection of one fluorescent signal in a channel setup for...environments. Since colocalization relies upon the detection of 2 independent fluorescent signals and their...practically speaking, for common lab microscopes the detection limit is 200 nm. The size of most proteins is ...is below 10 nm and therefore colocalization detected in microscopy cannot be interpreted as direct molecular...

...learning techniques to make sense of biology, with a special focus on gene editing techniques. References...Prime editing is a versatile genome editing technology that allows precise modifications of DNA (replacements...features and DNA repair determinants. Nature Biotechnology 2023, 1–11. https://doi.org/10.1038/s41587-023...and product purity by deep learning. Nature Biotechnology, 41, 1151–1159. https://doi.org/10.1038/s41587...efficiency across diverse chromatin contexts. Nature Biotechnology. https://doi.org/10.1038/s41587-024-02268-2 ...