...wanting to target specific neuron subtypes, since AAVs tend to be very neuro-permissive (Issa, 2023). Time...Postdoctoral Fellow in Connie Cepko’s Lab investigating neuronal circuits of the retina using viral tools.   References...

...think resources like this are why my brand of neuroscience has exploded. Because we have a resource like... visiting Addgene’s booth at the Society for Neuroscience 2023 conference. In 2020, the world changed ... recombinant antibodies service, part of the Neuroscience AntiBody Open Resource (NABOR) platform, supported...

... cells infected with AAV-Cre-DOG retain normal neuronal function, this system may make optogenetics in...domains. Both systems are easily adaptable to neuroscience applications, including optogenetics, and they...manipulation with GFP-dependent Cre recombinase.” Nat. Neurosci. 18(9) (2015): 1334-1341. PubMed PMID: 26258682...

...and Memory Formation. Neuron 79, 6 P1086-1093. https://doi.org/10.1016/j.neuron.2013.08.032.  Morita S...lentiviral backbones expressing blasticidin or puromycin resistance genes.  It’s that simple! Good luck...

...Rochester Medical Center! Patrick is equal parts neuroscientist, writer, and extreme sports enthusiast. He ...He recently obtained his Ph.D. in neuroscience from the University of Rochester Medical Center, where he...biology techniques to study inflammation and neurodegeneration. He now spends his time learning and writing...

...Chemical Synapses in the Brain. Neuron 68:843–856. https://doi.org/10.1016/j.neuron.2010.11.021  Gambarotto D...helped researchers gain a deeper understanding of neuronal synapses, centrosomes, parasites, and more (Bertiaux...

...Recombinant Antibodies in Basic Neuroscience Research. Curr Protoc Neurosci. 2020 Dec;94(1):e106. doi: 10.1002...PMC7665837. L'Abbé D, Bisson L, Gervais C, Grazzini E, Durocher Y. Transient Gene Expression in Suspension HEK293...

...experimental systems. They demonstrated LOV-Turbo in neurons, in pulse-chase experiments, and more. Find LOV-Turbo...element (TRE) or the cFos promoter. For example, neurons treated with a phorbol ester activator gave fluorescent...Images of iPAK4 fibers in cultured rat hippocampal neurons. HaloTag dyes are switched from yellow to red at...

...Technologies and Methods for Targeted Neuronal Manipulation. Frontiers in Neuroanatomy, 13. https://www.frontiersin.org...for Adeno-associated viral vector production. Neuromethods, 195, 77–101. https://doi.org/10.1007/978-1-...

...lab develops iPSC-based models of neurodegenerative and neurovascular diseases. He worked on the described... Dominik Paquet is currently a Professor for Neurobiology at the Institute for Stroke and Dementia Research...

...-dCas9-p300 Core, as well as pLV-dCas9-p300-P2A-PuroR for lentiviral expression. Figure 1: dCas9... deposited pdCas9-DNMT3A-EGFP and pdCas9-DNMT3A-PuroR for targeted cytosine methylation in mammalian cells... cells. Co-expression markers EGFP and PuroR enable sorting and selection of transduced cells. Grant Challen...

...generating zebrafish transgenic lines to study neurodegenerative diseases. Listen to Our Segement on the Tol...Plasmid 2: AAV Vectors for tageting GABA-ergic interneurons. Listen to Our Segment on these new AAV Vectors...

...Getting ready for Society for Neuroscience Conference 2023? So are we! Come say hi to us at Addgene's...Conference New to SfN? Try A Novice's Perspective on Neuroscience - SfN17!   ...

... connections between BLA projection neurons and mPFC interneurons can be labelled with an AAV1 Cre virus...

...empty backbones for shRNA expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids...negative control, and empty backbones such as pBABE-puro for retroviral expression and pLVTHM, a lentiviral...

...test recombination efficiency. When expressed in neuronal cells, each line showed broad recombinase-dependent...hamster ovary (CHO) cells, as well as in cortical neurons in layer 2/3 of the mouse brain. Rubin’s work shows...

... Rodrigo Arturo Rivera Solís, Universidad Autónoma de Yucatán, Mexico Dr. Rodrigo Arturo Rivera Solís...

...and chemogenetics communities to activate neurons and neuronal pathways using light and chemicals respectively...

...CD90.1 selection marker, Thy1.1, in addition to puromycin resistance.  The MYC-CRISPR library targets E-...AAV tools to selectively control parvalbumin interneurons from the Dimidschstein Lab More GCaMP8 ready-to-use...

...sodium-potassium pump expressed specifically in neurons of the brain. McSweeney says they now think that...that mutations in the gene may prevent neurons from returning to basal conditions normally after periods...