...site (MCS), but does not cut your insert, you could try using two different enzymes that have compatible ...

...do controls. See Tips and FAQ below for details. Try different vector to insert ratios to optimize the...

...highlighted in red above. When searching, it is worth trying alternate names, particularly for gene names. If...

...in an Open Source Lab Resources on Addgene.org Try the Plasmid Land Game ...

...sensitivity, which can be problematic if you are trying to show that your antibody can distinguish between...Additional resources on the Addgene blog:  Still trying to choose an antibody? Check out our Selecting ...This is a good option for applications like immunohistochemistry on human tissues, when (1) other strategies...correspond to protein levels, so you perform immunohistochemistry with your antibody on these tissues (A)....recognized by your antibody. You perform immunocytochemistry on these mutant cells as well as a non-mutant...epitopes of this protein.   You perform flow cytometry using both antibodies, as well as a third unrelated...sample using the antibody and then uses mass spectrometry to identify the captured proteins. If the antibody...

...one you use, of course, depends on what you are trying to do – there’s no “best” tool among them. While...best” gRNA depends an awful lot on what you are trying to do: gene knockout, a specific base edit, or ...Rees HA, Liu DR (2018) Base editing: precision chemistry on the genome and transcriptome of living cells...

...or two hanging out around the lab and especially try to attend a lab meeting. Observe how the lab members...scientific meetings (and present)? Making the decision Try to rotate or interview in more than one lab when ...(topic and/or technical aspects) applicable to industry if this path interests you  (e.g. human disease...drug/device development? Does the advisor have industry connections such as serving on Boards? Does the...the publication record show evidence of industry collaboration? If you are considering a career at a small...

...secondary antibody may be binding non-specifically – try optimizing your blocking and washing conditions or...are always new and improved variations you could try. We hope this post helped you start planning for ...referred to as immunocytochemistry (ICC) when the sample is cultured cells or immunohistochemistry (IHC) with....3001161 McLaughlin, K. (2019). Multiplexing Immunohistochemistry. Materials and Methods, 9. https://doi.org...antibodies available at Addgene Protocols for Immunocytochemistry Protocols for other antibody applications...

...unpublished evidence shows otherwise. Others will go on to try to apply these findings in their own work ultimately...likely that we’ll find a fluke when we’re simply trying to get our experiments to work, but part of good... sciences and featured speakers from academia, industry, nonprofits, and publishing. The livestream video...publishing community views reproducibility, how industry relies on reproducibility, and how you can push...

...comments below. And if you want to give GeoCas9 a try, as always, the plasmids are available from Addgene...

...Your Science Why Scientists Should Give Twitter a Try ...

...service of COVID-19 research? We decided to give it a try, by creating a service for COVID-19 researchers. ...

...plasmids out there Beyond these 3 big pushes, we try to increase your work’s visibility in a few other...

...with high marker expression level. Labs have been trying different approaches to improve CTC capture, but... is small, highly stable and has this amazing chemistry locking the domain together - no one knows what.... Many other Gram-positive bacteria have this chemistry in their surface proteins. It is best studied ...

...fewer and fewer people use plastic bags.  We also try to recycle our discards according to the Cambridge...plastic materials are fairly inexpensive in this country and we are quick to throw out chipped or imperfect...

... a microscope’s eyepiece can take some practice. Try to keep both eyes open and adjust the position of...

...actively testing it in other applications! If you try it out in a new assay, let us know. We’d love to ...

...failing, over and over again until you don't, of trying to understand your completely unexpected findings...

... the protein as it exists in your assay?  First, try to understand, to the best of your ability, what ...protein that is being used as a marker for flow cytometry, for example, can only be recognized by antibodies...samples. If, however, you are performing an immunohistochemistry assay, you may want to rule out monoclonal...

...particular sections. The focus groups allowed us to try out techniques that were discussed in the larger ...