...expresses multiple reprogramming factors driven by one promoter. While lentivirus has similar rates of reprogramming...control. Additionally, Sendai virus doesn’t rely on promoters for transcriptional regulation, so it requires... mini-plasmids. They contain only a eukaryotic promoter and the cDNA(s) to be expressed and they don’t...phosphatase, expression of embryonic proteins, and promoter demethylation and expression of endogenous pluripotency...

...either the Thy1 or CAG promoter, while Brainbow AAV is under control of the EF1a promoter. Livet et al., ...known as SpyLigase and is a protein domain that promotes the formation of an isopeptide bond between 2 ...insert fragments of DNA containing basic parts (promoters, UTRs, coding sequences, terminators, etc) into... creating a single transcriptional unit (Ex: a promoter, 5’UTR, coding region, and terminator). Next, ...individual types of pre-cloned insert modules (plant promoter, N-terminal tag, coding sequence of the gene of...

... Prior to engineering a transgenic strain, the promoter being used should be tested in cell lines to determine...with expression controlled by a tissue-specific promoter). And, Cre may also be either constitutively expressed...

...vector containing homologous ends to the breakpoint promotes homologous recombination of the targeting vector... process. The addition of small molecules that promote homologous recombination or the use of long single-stranded...

...neuron-specific expression (hSyn promoter) and pan-mammalian cell expression (CAG promoter). An improved version ...

...very realistic. Envisioning GFP as a substrate promoting dimerization, Tang et al. tested pairs of their... they activate gene expression from different promoters. Moving to an in vivo system, Tang et al. electroporated...

...CRISPR, Addgene gave to me: a method to capture promoter-enhancer 3D chromatin structures using CRISPR....

...components, such as your CRISPR enzyme or Pol 2 promoter. The options available in each class, or module...out reporters, Cas proteins, guide cassettes, promoters, N’ and/or C’ terminus, and selection markers....

... the pYES2.1 plasmid is controlled by the GAL1 promoter. The cloning procedure adds both V5 and His6 epitope...grow in the absence of methionine. Since the GAL1 promoter controls MET/Met gene expression, complementation...

...well as several derivatives encoding alternative promoters and/or fluorescent tags are available at Addgene...transcriptional control of a strong constitutive promoter (BBa_J23100) and expresses N-demethylases and ...

...recombinase. If GFP is under control of an endogenous promoter, you can use expression GFP to track cells participating... physiopathological events to which the chosen promoter responds. You can also use this method to tag ...

...expression vector Promoter driving Cas9 expression can be constitutive or inducible. U6 promoter is typically...

...under the control of the powerful mammalian CMV promoter. Envelope proteins are a key component of lentiviruses...

...to measure time. These plasmids with different promoters driving expression of Cas9 were used in this study...ready-to-use AAV vectors expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with...

...expresses S. pyogenes Cas9 from the PGK promoter) and U6 promoter-driven guide RNA constructs, pAdSh.U6....

... toxin gene (regulated by a rhamnose-inducible promoter) and a chromoprotein gene. Allelic exchange occurs...it is under the control of a rhamnose-inducible promoter. The resolution step leads to the desired mutation...

...allows for customization with strain-specific promoters, antibiotic resistance selection markers, gRNAs...nature allows for the customization of guides, promoters, selection markers, and different versions of ...

...studies have shown that one base change in the promoter region of gamma hemoglobin (HGB1/2) leads to continuous.... ABE8 efficiently edited the fetal hemoglobin promoter and caused persistent expression of fetal hemoglobin...

...Addgene are perfect for expressing GFP from a given promoter, and the technique can be adapted to other transgenes...observed will be dependent on both the endogenous promoter and the 3’ UTR of the locus of interest, since...

...plant lab, so it includes everyone’s favorite promoters, plus a few new ones that are not used so much...know how to start. They might want to use our promoters and terminators, but then they aren’t sure how...