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We narrowed to 301 results for: pET

Showing: 121 - 140 of 301 results

  2. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...-mimetic sequences that imitate this motif and compete with both small molecule integrin inhibitors and...their targeted integrin subtype, even when in competition with other similar integrins, as reported by ...

  4. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...your primers should be free of palindromic and repetitive sequences, but if present, a minor extension ...template should be isolated from a methylation competent bacterial strain (e.g. DH5α which is dam+). Methylation...

  5. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...equipment, categorize all of your minor tools, like pipets, or mini-centrifuges, etc. Be sure to capture as...phones.  As for reagents and consumables (such as pipet tips, glassware, etc), get a simple list from your...

  7. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...experiences! These applications can be highly competitive, so asking for help from mentors and other students... Imposter: The Effect of Perceived Classroom Competition on the Daily Psychological Experiences of First-Generation...

  8. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...with fluorescent derivatives of its substrate trimpethoprim (TMP-tag) (Miller et al., 2005). Although this...Benzylguanine (BG) Many Easy eDHFR 159 Trimpethoprim  (TMP-tag) Few Easy HaloTag 295 ...

  11. Typing CRISPR Systems

    Type
    Blog Post
    ...hypotheses that they are involved in plasmid competition or can hijack machinery from other CRISPR systems...CRISPR–Cas systems are highly diverse and involved in competition between plasmids. Nucleic Acids Research, 48(...

  12. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...Unnatural amino acid incorporation in mammalian cells Peter Schultz and colleagues at the Scripps Research Institute...Giannaks et al 2003, Bert et al 2000). In 2000, Peter Cockerill and his lab developed an improved luciferase...available, as well as 5S RNA F30-2xdBroccoli fusion and pET28c-F30-Broccoli controls. Don’t just eat more broccoli...

  14. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...activity than BE3; editing window ~1-2 nt  87438  pET42b-HF-BE3  HF-Cas9 (NGG)  Very low off-target activity...et al. Cell Systems. 2016. PubMed PMID: 28189581 Peters J, et al. Cell. 2016. PubMed PMID: 27238023 Listen...

  16. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ...submission.    Our Cultured Warrior (made by Nate using pipette boxes, wire, cable ties) received a festive makeover...

  18. Deck the Lab 2022!

    Type
    Blog Post
    ... world. Cap your Eppendorf tubes, hang up your pipettes, and seal up those plates – it’s time to turn ...

  20. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...organization we thrive on 3 things: fun, teamwork, and competition. And our Halloween celebration this Friday will...
Showing: 121 - 140 of 301 results