...Vectors Read about Reporter Gene like Luciferase and GFP Resources on Addgene.org Find Plasmids for Your...promoter present in your plasmid backbone must to be compatible with the type of RNA that needs to be made: if...

...liver injury markers were not increased compared to a control AAV-GFP. Not only did the AAV-SaCas9-sgRNA constructs...editing is the potential for off-target effects. To compare the off-target effects of SpCas9 and SaCas9, Zhang...had modest editing rates in HEK-293T cells when compared to the full-length SpCas9. In 2016, Chew et al...cleavage of HBB and CCR5 genes in human cells using compact expression cassettes. Sci Rep 5: . https://doi....

... not endogenously-expressed (e.g., using an anti-GFP antibody), the target antigen is first transiently...containing 1 mM sodium azide. This buffer is not compatible for use in live cells and will interfere with...secondary antibody. The fluorescence pattern is compared to an untransfected control. Immunohistochemistry...secondary antibody. The tissue labeling pattern is compared to the mRNA expression pattern cited in the Tissue...HRP-conjugated secondary antibody. Protein expression is compared to an untransfected control and the protein size...

...aptamers able to bind to a small molecule derivative of GFP called 3,5-difluoro-4-hydroxybenzylidene imidazolinone...identified through microfluidic-assisted in vitro compartmentalisation for their high binding affinity to TO1-biotin...of these variants exhibit increased brightness compared to Mango, and all are photostable and useful for...

...Root 105533 pAAV.CMV.Luc.IRES.EGFP.SV40 Firefly CMV AAV expression of firefly luciferase and GFP James Wilson...phGluc Gaussia EF1α Expression of Gaussia luciferase; GFP is expressed if cells are infected with virus Christopher...Firefly TGB AAV expression of firefly luciferase and GFP James Wilson 106457 pCR3.1-Luc Firefly CMV Mammalian...Firefly luciferase. Plasmids are Gateway cloning compatible. Learn more about DULIP in our 27 Hot Plasmids...

..., Hess et al. successfully evolved wild type GFP to EGFP using CRISPR-X and subsequent FACS sorting. They...with unique tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many ... of DsRed connected to a pH-sensitive variant of GFP (SEP) by a 9 amino-acid linker (see Figure 1). The...several GMAP-compatible vectors for a wide variety of biological applications. First, GMAP-compatible backbones...including a stop codon) formats. The collection is compatible with both standard Gateway cloning and the FNLCR...sites. For instance, the authors generated a GMAP-compatible backbone containing Rosa26 homology arms. The...GMAP, there will be a continuous expansion of compatible backbones, promoters, and genes available to ...

...experiment, consider co-transfecting with GFP. This allows you to sort for GFP-positive cells and to enrich for...system, developed by the Zhang lab, which has comparable efficiency to the optimized chimeric design but...Where are double strand breaks (DSBs) induced, compared to where the target site sequence (protospacer...how closely homologous the off-target sites are compared to the on-target site, the specific site sequence...

...typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive cells, or selection...transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify and enrich positive...repair template and displays reduced efficiency compared to NHEJ knockout. Base editors can make a limited...our AAV Guide ) ∼4.5 kb packaging limit (only compatible with smaller Cas enzymes) Transient or stable...Cas9 and gRNA Choose a delivery method that is compatible with your experimental system. CRISPR efficiency...

... includes things like viral vectors that deliver GFP.   Figure 3: A) Cumulative AAV preps available..., and jGCaMP8 viral preps distributed per year compared to other AAVs preps from 2016–2023. B) Inset showing...

...England Biolabs website. For a comprehensive comparison to GFP, please refer to this table. Technology SNAP-tag...internal or surface proteins Single constructs are compatible with multiple applications (different fluorophores...

...are generated by inserting a circularly-permuted GFP into an intracellular loop of various G-protein-coupled...enhanced brightness, expression, and sensitivity compared with earlier SF-iGluSnFR. Image from Aggarwal ...size and reduced sensitivity to pH and calcium compared to previous lactate biosensors, and its affinity...

...find antibody plasmids for: Common antigens such as GFP or mCherry Monoclonals, Nanobodies, Sybodies, or ...peptide, from the popular SunTag system, fused to sfGFP for imaging. Learn more about antibodies and their... and Evolving Functional Proteins. METHODS: A Companion to Methods in Enzymology 8, 94–103 (1995). Carlos...

...pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 CAG Archon1 EGFP Cre dependent 5 Boyden 115892 pAAV-Syn-Archon1-KGC-GFP-ER2 Syn Archon1...Archon1 EGFP Constitutive 8 Boyden 115893 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] Syn Archon1 EGFP Cre dependent...Podgorski Calcium Sensor: CaMPARI 100831 pENN.AAV.CAG.Flex.CaMPARI.WPRE.SV40 CAG CaMPARI none Cre dependent ...pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Syn CaMPARI2 his Constitutive 1 Schreiter 101061 pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40...dependent 5 Looger 100832 pAAV.hSyn.CaMPARI.WPRE.SV40 Syn CaMPARI none Constitutive 1, 5, 9 Looger 101060 pAAV_hsyn_NES-his-CaMPARI2...F391W-WPRE-SV40 Syn CaMPARI2-F391W his Constitutive 1 Schreiter 101064 pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40...HaloCaMP1a 138327 pAAV-synapsin-HaloCaMP1a-EGFP Syn HaloCaMP1a EGFP Constitutive 1, 9 Schreiter Calcium Sensor...

...fluorescent protein that could be co-imaged with GFP using the same wavelength for excitation. The result... a good story (1). From a deep sea shrimp to a compact powerhouse of bioluminescence, NLuc is 100-fold...yield, brightness and improved maturation time compared to similar orange fluorescent proteins. In their...more than 2-fold brighter signal from Antares as compared to Fluc in this model. The construct for Antares...transfer of energy from one fluorescent protein to a compatible fluorescent protein. FRET can be measured by ...

...SYFP2 Clover Green Mammalian Expresses Clover (a GFP variant) commonly used with mRuby2 pLSSmOrange-N1...to Amber via a 32 amino acid linker mGFP-10-sREACh-N3 Monomeric EGFP attached to super-REACh via a 10 amino...to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian Expresses mammalian... Clover pGWF1 Cyan & Yellow Bacterial Gateway-compatible vector to express a gene of interest fused between...

...157970 pTR-UF11 chimeric CMV/Chicken Beta actin (CBA) GFP Control Sergei Zolotukhin Citation Information AAV2...intravitreal injection in mice and marmosets as compared to the AAV2 serotype. The AAV2(Y444F) serotype...in the retina following subretinal injection as compared to the parental AAV44.9 serotype. It was developed...acknowledge Shannon Boye and cite Boye, et al. 2016. Impact of Heparan Sulfate Binding on Transduction of Retina...

...Church lab uses this system to control expression of GFP.)  To turn this inducible system into a repressible... cells and ~24,000-fold in human HeLa cells as compared to basal expression. Co-expression of repressor...

... pU6-sgGFP-NT1 Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GFP (NT1)...pSLQ1658-dCas9-EGFP Human expression vector containing dCas9 that is fused to 2x NLS and EGFP for CRISPR ...targeting endogenous CD71 gene 46919 pMLS-SV40-EGFP Target EGFP gene that is stably integrated into HEK293...complementary region. The uniqueness of CRISPRi, as compared to several recently published works on applying...

...labeling. Size is also a factor. Bulkier tags, such as GFP, may interfere with protein expression or function...realizing it. Yet, this selection is crucial as it will impact downstream outcomes of an experiment, including...increased versatility in probe attachment, and compatibility with far-red and near-infrared fluorophores....Grimm & Lavis, 2022). These variations directly impact the way you can utilize these dyes. The most-used...may prove challenging to stain intracellular compartments in in vivo model systems, so extra care must...emission of labeled agonists and antagonists, impacting the interpretation of experimental results. It's...dopamine D2/D3 receptors (Allikalt et al., 2020) Impact of labeling process: The process of dye labeling...

...typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive cells, or selection...transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify and enrich positive...repair template and displays reduced efficiency compared to NHEJ knockout. Base editors can make a limited...our AAV Guide ) ∼4.5 kb packaging limit (only compatible with smaller Cas enzymes) Transient or stable...Cas9 and gRNA Choose a delivery method that is compatible with your experimental system. CRISPR efficiency...