...DNA. Add the diluted PEI dropwise while gently flicking the diluted DNA tube. Incubate the mixture 15–...

... 3:1 ratio is not working or when doing more complicated cloning. While 3:1 will get you in the ballpark...

...Link opens in a new window) . Gibson Assembly® is licensed to New England Biolabs by TelesisBio. Gibson DG...

...as methanol or acetone may be better for some applications. Remove the paraformaldehyde and follow your...

...cells and will interfere with conjugation. For applications such as these, the buffer can be removed by ...tissue panel in one of the antibody’s recommended applications. The specific QC experiments performed varies...

...Scientists using these zinc finger arrays in a publication should notify [email protected] and...acknowledge NIH grant number R01 GM088040 in the publication. References Rapid 'open-source' engineering of...

...lentiviral preparation allows you to control the multiplicity of infection (MOI) in downstream studies. This...

...examples of plasmid types available for each gene. Click the gene name for a full list of plasmids available...

...of certain genes that are required for viral replication. Thus, as a safety feature, viruses used in research...Instead, the genes that are required for viral replication are usually divided among many plasmids, such...

...superior for less favorable protospacers and applications requiring particularly tight binding. In these...advisable to use the consensus sequence. See the publication for details. A protocol for synthesizing gRNAs...

...information on determining DNA concentration and purity click here . Top Protocol: Kit-free Alkaline Lysis Plasmid...

... of a heat resistant DNA polymerase from the thermophilic bacterium, Thermus aquaticus (Taq) . Taq polymerase...

...-25% of all human cancers. Ras Pathway Plasmids Click on a name to find available plasmids for the gene... Research Return to top Ras Pathway - Gene List Click on a name to find available plasmids for the gene...

...loci seem to be associated with the disease and implicate a wide range of cellular processes. The vast majority...Rep. 2023 Aug 30. Enhance differentiation to mesencephalic dopaminergic (mesDA) neurons using gene knockout...

...lower dilutions depending on your downstream applications. If you’ve titered your virus beforehand, you...

...Information When using the Caltech serotypes in future publications, please acknowledge Viviana Gradinaru and cite....PHP.N with GRE or transgenic animal For PNS applications in rodents and NHPs: Broad tropism - AAV-MaCPNS2...

...IgG2a 114496 Anti-KCNT2/Slo2.1/Slick K+ channel [N11/33R] KCNT2/Slo2.1/Slick K+ channel Mouse Mouse IgG2a...extensively validated for neuroscience research applications. A substantial number of these have been cloned...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene...opens in a new window) UC Davis/NIH NeuroMab Publications (Link opens in a new window)...

...quantification. This protocol was modified from the publication Lock et al. (2014) (Link opens in a new window...

... layers are covered with media. 293T cells are delicate and detach very easily - media should always be...

... sample. This protocol was modified from the publication Wang et al. (2018) . Before Starting Thaw the...