...specificity and binding affinity, can be produced in E. coli, and are both soluble and easily engineered.  Monobodies...

... students to propagate the plasmids in either E. coli (ori and ampR) or S. cerevisiae (2µm ori and URA3...

...infections. The Cas3 enzymes target several strains of E. coli and lethally chop their genomes into many, many ...

...CRISPR libraries that target mouse, human, fly, E. coli, and T. gondii genes.  2) What genetic modification...

...programs to engineer 14 chromoprotein sequences into E. coli BioBrick plasmids (meffRed, eforRed, asPink, spisPink...

...thetaiotaomicron, Mycobacterium, Pseudomonas, and E. coli. However, many non-model bacterial species lack ...

...is to express and purify His-tagged Cas9 from E. coli using a plasmid such as pET-28b-Cas9-His from Alex...

...transformation amplification protocol is the Chong Zhang E. coli Genome-wide Inhibition Library. DNA and cell amounts...

...  Most Addgene plasmids are maintained in the E. coli lab strain DH5a, but others may require strains ...

...to use in an experiment. E. coli strains for propagating plasmids E. coli are gram-negative, rod shaped...Addgene blog posts relating to common E. coli lab strains and E. coli strains specialized for protein expression... with Plasmids Introduction Types of Plasmids E. coli strains for propagating plasmids Antibiotics commonly...For instance, most plasmids are replicated in E. coli and are relatively small (∼3000 - 6000 basepairs.... When a plasmid exists extrachromosomally in E. coli , it is replicated independently and segregated ...many different naturally occurring strains of E. coli , some of which are deadly to humans. The majority...majority of all common, commercial lab strains of E. coli used today are descended from two individual isolates...

...single-copy gene expression in E. coli TALENs Gene targeting TALEN kits - Construct a ...

...and activation). Genome support for bacteria ( E. coli, B. subtilis ), yeast ( S. cerevisiae ), worm ( ...

...fluorescence-activated cell sorting (FACS) of E. coli transformed with aptamer libraries, the Jaffrey ...nucleotide aptamer they called Broccoli. As a product of SELEX and FACS, Broccoli is inherently more stable...new RNA-based scaffolds to contain and stabilize Broccoli cassettes, thereby extending their use into mammalian...mammalian cells. They’ve also dimerized Broccoli to further enhance its biosensor capabilities (see Fig...et al. “In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies....Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene View All Fluorescent Protein...

...Activity Learn more about protein tags Read about E. coli strains for protein expression Additional Resources...

...Nanobodies are easily expressed in the cytosol of E. coli, however the nanobody proteins tend to get stuck...inclusion body problem and makes nanobody production in E.coli as simple as following a DNA miniprep protocol!... were expressed in SHuffle T7 Express competent E.coli (NEB) using IPTG induction. To biotinylate the ...

...propagated in the endA-mutated NEB Stable strain of E. coli. In addition, plasmids are typically prepared using...

...Berkmen, M. (2012). SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding...

...purification process simpler. Due to its high yields, E. coli is often used for the production of Fab fragments...

...strep-tag and, more recently, OLLAS (Escherichia coli OmpF linker and mouse langerin) and Sun Tag (Viswanathan...

...beta-lactam and quinolone resistance genes in E. coli using plasmid and phagemid delivery systems. Phagemids...