Skip to main content
Addgene
Showing: 1 - 20 of 26 results

  1. Open Enzyme Collection

    Type
    Collection
    ...pOpen-Endonuclease IV E. coli E. coli Endonuclease IV 165561 pOpen-Endonuclease VIII E. coli E. coli Endonuclease ...Endonuclease VIII 165571 pOpen-Exonuclease III E. coli E. coli Exonuclease III 165559 pOpen-T5gene12/13 T5 Exonuclease... pOpen-T4gene30 T4-DNA Ligase 165564 pOpen-ECOligA E. coli DNA Ligase 165569 pOpen-Tthlig Tth DNA Ligase...Open Enzymes parts for expression in Escherichia coli bacteria. Researchers can use the collection as ... vector of their choice and transforming into E. coli bacteria. This can help reduce lab costs and overcome...vector of their choice and then transform into E. coli for expression and purification ( pET28a-LIC or ... . Open Enzymes are also compatible with the E. coli Protein Expression Toolkit (Link opens in a new ...

  2. Microbiology Resources

    Type
    Collection
    ...set : E. coli gene expression - Endy Lab CIDAR MoClo Parts Kit : Molecular cloning for E. coli - Densmore...plasmids is a bacterial stab (of a laboratory E. coli strain). Addgene continues to monitor its library...export laws and biosafety regulations. Looking for E. coli or yeast plasmids? Addgene has a wide variety of... Fluorescent Proteins FRET-based biosensors : E. coli expression - Pertz Lab Bacterial Fluorescence Labeling...pCri System : Heterologous protein expression in E. coli, B. subtilis and P. pastoris - Gomis-Rüth Lab pOSIP... purification and cell-free circuit design in E. coli - Freemont Lab MoClo-YTK : Assembly of single and...CRISPathBrick Multiplex Plasmid : CRISPR expression in E. coli - Koffas Lab Additional Addgene Resources Synthetic...

  3. Genetic Code Expansion

    Type
    Collection
    ...tyrosyl-tRNA synthetase E. coli pBof Mammalian TAG Huiwang Ai 68292 SepOTSλ SepRS9 E. coli Sep Bacterial TAG Jesse... E. coli photocaged cysteine Mammalian TAG Huiwang Ai 73544 pEvol-pAcFRS.2.t1 pAcFRS.2.t1 E. coli p-acetyl-l-phenylalanine...synthetase E. coli Mammalian TAG Simon Elsaesser 140019 pAS_4xEcoLeuT(CUA)_AnapRS AnapRS E. coli azido-phenylalanine... first with a control reporter gene – GFP for E. coli or mCherry-GFP for mammalian cells. You should also...Bacterial TAG Peter Schultz 48696 pANAP AnapRS E. coli fluorescent AA, Anap Mammalian TAG Peter Schultz...pAcBac2.tR4-OMeYRS/GFP* tyrosyl-tRNA synthetase E. coli various unnatural amino acids Mammalian TAG Peter...Mammalian TAG Peter Schultz 51401 pAM1 NLL-MetRS E. coli azi-donorleucine (Anl) Bacterial ATG David Tirrell...

  4. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...,170 Bikard Lab E. coli Genome-wide Inhibition Library v1 115927 Inhibition E. coli Bikard N/A 19 92,919...Bikard Lab EcoWG1: E. coli Genome-wide Inhibition Library v2 131625 Inhibition E. coli Bikard N/A ∼5 21,417...Zhang E. coli Genome-wide Inhibition Library 113134 — 113138 113146, 113147 Inhibition E. coli Chong Zhang...Other Species Human Mouse Fly Yeast T. gondii E. coli S. pneumoniae M. tuberculosis M. smegmatis Green...Protospacer flanking site (PFS) Library 79153 Knockout E. coli Zhang N/A N/A N/A - The protospacers contained in...

  5. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...phosphorylated amino acid to the genetic code of E. coli (Park et al., Science 2011). Follow up work from...the parent protein. Using the recoded strain of E. coli ( 68306 ) with the optimized phosphoserine orthogonal...flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Pirman...Enhanced phosphoserine insertion during Escherichia coli protein synthesis via partial UAG codon reassignment...described in: Expanding the genetic code of Escherichia coli with phosphoserine. Park HS, Hohn MJ, Umehara T,...lab published a new strain of recoded E.coli (rEcoli XpS 192872 ) and an optimized phosphoserine orthogonal...phosphoprotein expression in rEcoli XpS in the Methods section link below (Methods for iSPI, rEcoli XPS, pSerOTS-C1...

  6. Bacterial Expression Systems

    Type
    Collection
    ...Plasmids 101: E. coli strains for protein expression Plasmids 101: Common Lab E. coli Strains Why Bacteria...Bacteria? E. coli are commonly used to store and replicate plasmids of all types, but beyond that, researchers...researchers also use bacteria like E. coli and their relatively well understood biology to answer many interesting... an N-terminally His-tagged TALEN fused to an E. coli codon optimized FokI endonuclease domain. pET-Sangamo-His...of a C-terminally His-tagged TALEN fused to an E. coli codon optimized FokI endonuclease domain. Return...can be used to express a protein of interest in E. coli , B. subtilis and P. pastoris . All plasmids use...

  7. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...BsaI E. coli, S. pneumoniae Kanamycin none, need Cas9 plasmid Marraffini 42876 pCas9 BsaI E. coli, S. pneumoniae...

  8. Tetracycline Inducible Expression

    Type
    Collection
    ...eukaryotes. At the time, bacterial systems from E. coli presented the best candidates for inducible expression... absence of binding tTA or rTA; has high copy E. coli origin None Either Ralser 64238 pTet-IRES-EGFP Lentiviral...

  9. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Repeat) sequences were initially discovered in the E. coli genome in 1987, but their function as a safeguard...Mammalian systems (Human, mouse, and rat) Bacteria ( E. coli , Streptococcus , Streptomyces , and others) Drosophila...alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product. J Bacteriol...

  10. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...reaction (see step 3.2 ). Transform 10 μl of DH5α E. coli cells with 1 μl of reaction (from 3.2.1 - 3.2.2 ...plasmid vector. Transform the plasmid into DH5α E. coli cells and plate onto LB agar plates with the relevant.... PubMed . Transformation of plasmid DNA into E. coli using the heat shock method. Froger A, ...

  11. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...created by directed evolution of the Escherichia coli TadA, a tRNA adenine deaminase. Like cytosine base...

  13. Viral Production

    Type
    Collection
    ...propagated in the endA -mutated NEB Stable strain of E. coli . In addition, plasmids are typically prepared using...

  14. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...3xFLAG-dCas9/p-bacteria Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis...

  17. Validated gRNA Sequences

    Type
    Collection
    ...article 60224 cut S. pyogenes 25263330 Zhang LacZ E. coli TGCGAATACGCCCACGCGAT 60225 cut S. pyogenes 25263330...ACTCCAGTCTTTCTAGAAGA 64216 cut S. pyogenes 25803306 Kuhn rpsL E. coli AAAAAACCGAACTCCGCGCTGCGTAAAGTA 44505 cut S. pyogenes...AAGTTCGTATGGAAGGTTCCGTTAA 74075 interfere S. pyogenes 26829286 Lu LacZ E.coli CCCGAATCTCTATCGTGCGG 74179 cut S. pyogenes 26627737...

  18. COVID-19 Resources

    Type
    Collection
    ...plasmids. The collection contains: Plasmids for E. coli and Yeast expression Spike protein constructs for...

  19. Cre-lox system

    Type
    Collection
    ...intron, preventing Cre expression when cloning in E. coli . This alteration enables the generation of a single...construct (unmodified Cre will cause recombination in E. coli , deleting the floxed portion of a construct during...

  20. CRISPR Guide

    Type
    Collection
    ...created by directed evolution of the Escherichia coli TadA, a tRNA adenosine deaminase. Like cytosine ...the DNA and replaces it with another base. In E. coli , this strategy yields C to A base edits, whereas...harnessed for antiviral mechanisms in bacteria ( E. coli ). Like the other Cas enzymes, the Cascade-Cas3 .... (2018). Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors....
Showing: 1 - 20 of 26 results