... rare genetic eye disease, Leber’s congenital amaurosis. This is the first of what I, scientists, doctors...childhood-onset form of blindness name Leber’s Congenital Amaurosis (LCA). Shortly after the cloning of the RPE65 ...efficiently transduce various types of retinal neurons. Nowadays a toolbox of naturally occurring and ...muscular dystrophy, spinal muscular atrophy, neurodegenerative diseases and metabolic diseases are also underway...

...expressing validated antibodies for neuroscience research (see the NeuroMab Collection for details). The following..., I wanted to find a specific plasmid from the NeuroMab collection for an antibody against PARIS, a zinc...zinc finger protein involved in neurodegeneration in Parkinson’s disease. Knowing the primary investigator...

...Domains Tweeted by Biologists. 7. Biologists, neuroscientists and ecologists (re)tweet scientific domains...Domains, Categorized by Discipline. Biologists, neuroscientists, and ecologists (orange box) post tweets containing...however, ~20% of life scientists  (biologists, neuroscientists, ecologists) tweet scientific domains 20% or...

...image the calcium response in the dendrites of T5 neurons by placing the calcium indicator GCaMP6f in the...optogenetically control the depolarization of R2 neurons using CsChrimson::tdTomato, an optogenetic tool...projection-specific brain-wide reconstruction of single neurons. Nature Methods 15:1033–1036 . https://doi.org/...

...heard of a Freshers Fair? If you’re from outside of Europe, the answer is probably no, but these fun and informative... of the main ways budding young scholars across Europe get introduced to the cultures and opportunities... and scientific community activities throughout Europe and the world. So get in touch! Resources at Addgene...

...marker such as puromycin, you can (also) select for the positive colonies by puromycin selection. You ...transfected cells (for example, plasmid with a puromycin resistance cassette, such as PX459). After you...

...iPSCs) to take on a “neuron-like” phenotype when targeting genes involved in neurogenesis, which had proven...EGFP, mCherry, iRFP, tdTomato, luciferase, LacZ, puromycin or CreERT2 can be combined with custom-cloned ...a separate reporter gene (EGFP, iRFP, IFP1.4, puromycin, neomycin or luciferase) to create a final lentiviral... voltage indicator for use in the detection of neuronal action potentials. The Cohen lab wished to improve...the genome. In this work, they use AAVS1_Puro_PGK1_3xFLAG_Twin_Strep and nuclease driven recombination ...

...Technologies and Methods for Targeted Neuronal Manipulation. Front Neuroanat 13: . https://doi.org/10.3389/fnana...expression, while the human synapsin 1 promoter is neuronal specific (Haery et al., 2019). Most AAV transfer...

...already available allowing you to chemically control neuronal activity while studying your phenotype of interest...interest. Optogenetic tools for similarly controlling neuronal activity with light will be available soon. We...we have retrograde AAV that you can use to map neuronal connections. Expanding the viral vector knowledge...

... Frank Graham showed HEKs display many immature neuron-like qualities, which is inconsistent with kidney...is next to the kidney and is known to have some neuronal qualities.) While HEKs probably aren’t a great..., et al., Preferential transformation of human neuronal cells by human adenoviruses and the origin of ...

...genes and wanted to express them within a specific neuronal compartment, you could express them individually...J. C. Kagan, Environmental Stress Causes Lethal Neuro-Trauma during Asymptomatic Viral Infections, Cell...binary transcriptional systems in Drosophila., Nat. Neurosci. 9, 703–709 (2006). PubMed PMID: 16582903. 8. ...

...started yet! @neurosymp will be interesting this year. Everyone interested in #Neuroscience should come ...networking within the app. For example, the NeuroSymposium had virtual tables that you can join to connect...

...proteins will be tested. Investigating changes in neuronal activity after ischemic brain injury Dr. Gohar... are urgently needed. Therefore, understanding neuronal networks, their interactions and information processing...microscope will allow Tsakanova to image cortical neurons and study changes in neural activity after ischemic...

...channels that can be used to silence the activity of neurons. Earlier this year, the Hegemann lab and collaborators... cysteine.  With the introduction of MerMAIDs, neurons can be turned off quickly and with high precision... a valuable optogenetics feature when studying neuronal activity. The researchers identified MerMAIDS ...

...region and ~75% of neurons were co-transduced with Cpf1 and GFP.  Of these neurons, ~15% had indels at...multiplex edit 3-4 genes in a primary culture of mouse neurons and in vivo. For these experiments, cells were ...

...Deficiency (CTD) is a rare X-linked inherited neurometabolic condition and one of three Cerebral Creatine...affected signaling pathways and how they lead to neurological phenotypes observed.” Using genetically-encoded...genetically-encoded biosensors and iPSC-derived cortical neurons from patients with GNB1 mutations, the lab hopes to...

...wanting to target specific neuron subtypes, since AAVs tend to be very neuro-permissive (Issa, 2023). Time...Postdoctoral Fellow in Connie Cepko’s Lab investigating neuronal circuits of the retina using viral tools.   References...

... cells infected with AAV-Cre-DOG retain normal neuronal function, this system may make optogenetics in...domains. Both systems are easily adaptable to neuroscience applications, including optogenetics, and they...manipulation with GFP-dependent Cre recombinase.” Nat. Neurosci. 18(9) (2015): 1334-1341. PubMed PMID: 26258682...

...and Memory Formation. Neuron 79, 6 P1086-1093. https://doi.org/10.1016/j.neuron.2013.08.032.  Morita S...lentiviral backbones expressing blasticidin or puromycin resistance genes.  It’s that simple! Good luck...

...Rochester Medical Center! Patrick is equal parts neuroscientist, writer, and extreme sports enthusiast. He ...He recently obtained his Ph.D. in neuroscience from the University of Rochester Medical Center, where he...biology techniques to study inflammation and neurodegeneration. He now spends his time learning and writing...