... knockout.
If knockout samples are unavailable, try substituting with knockdown approaches using siRNA...weak, then it is best to reassess the protocol and try to find a way to reduce, if not eliminate, the noise...W. “Controls for Immunocytochemistry.” Journal of Histochemistry & Cytochemistry, vol. 59, no. 1, ...al. “Controls for Immunohistochemistry.” Journal of Histochemistry & Cytochemistry, vol. 62, no. 10...that the target protein was measured on.
Flow cytometry
In addition to the controls listed above, viability...compensation controls are recommended for flow cytometry. A viability control allows you to detect dead...fluorophore and are critical to analyze multiplex flow cytometry experiments.
We hope that this blog post will ...