...GAGTCCGAGCAGAAGAAGAA 47508 cut S. pyogenes 23792628 Joung ETC2, TRY, and CPC A. thaliana 71288 cut S. pyogenes 26193878...

...addition to doing some of the quick things above, try joining or starting a sustainability project. This...

... The PAM sequence is of particular concern when trying to edit a gene using homology directed repair, ...of reagents available through Addgene! If you're trying to decide which CRISPR tools are best for your ...

...lentiviral preps unless absolutely necessary. Many labs try to use freshly collected lentivirus for downstream...well to other cell types. To overcome this issue, try transducing with a range of dilutions of your lentiviral...detection kits that use detector cell lines, ELISA, fluorometry, or PCR-based approaches to detect even low levels...

... associations across different universities are trying to compile more comprehensive demographic information...beyond those required at the lab bench. At Addgene we try to do our part in boosting awareness of various career...

...to probe interactions in locations of interest.  Try BioID for your proximity labeling experiment! APEX...favorite protein or RNA motif, give these methods a try.  References:  Branon, Tess C., et al. "Efficient...antibody based affinity purification and mass spectrometry approaches. Recently however, scientists have...classic antibody based affinity purification/mass spectrometry methods.  BioID: a promiscuous biotin ligase...purified using streptavidin and separated via flow cytometry.  Find plasmids for EXCELL here! Since BioID was...

...the world so it feeds a lot people. This lab is trying to improve homologous recombination efficiency ...injection into adult female mosquitoes. Now they are trying to replace embryo injection (hard to do, expensive...

...taking a look at what works and what doesn’t, and trying all over again. Here’s that process!   Figure...Shot without anyone else in the office.    I try to build every setup with an eye to the future. I...

...Jennifer Doudna’s lab analyzed metagenomic datasets to try to determine whether simpler and maybe smaller Cas...

...online is a little trickier -- but at Addgene, we try to make the process as smooth and simple as possible...

... should also be able to understand what you are trying to convey without having to extensively use Google...

...Assembly Kit! Tips and tricks For those interested to try the PUF Assembly Kit out for themselves, she does...

... does have one special tip for anyone looking to try this at home in their own lab: be very careful with...

...growth after overnight incubation. What went wrong? Try growing the culture for more time. Some bacterial...following into a 500 mL glass bottle: 4 g NaCl 4 g Tryptone 2 g Yeast Extract and dH 2 O to 400 mL Note: If...

...for career skills. Download a summary PDF here or try out the online Researcher Development Framework Planner...lab that has multiple collaborations, even with Industry labs Work on a big project that relies on a division...inviting others (be on a speaker committee) Follow industry publications like FierceBiotech to “learn the ...

... to have as much context as possible. When I am trying to resolve a conflict between two different teams... of conflict resolution. In compromise, you are trying to come to a consensus that all conflicted parties...

... knockout.  If knockout samples are unavailable, try substituting with knockdown approaches using siRNA...weak, then it is best to reassess the protocol and try to find a way to reduce, if not eliminate, the noise...W. “Controls for Immunocytochemistry.” Journal of Histochemistry & Cytochemistry, vol. 59, no. 1, ...al. “Controls for Immunohistochemistry.” Journal of Histochemistry & Cytochemistry, vol. 62, no. 10...that the target protein was measured on.    Flow cytometry In addition to the controls listed above, viability...compensation controls are recommended for flow cytometry. A viability control allows you to detect dead...fluorophore and are critical to analyze multiplex flow cytometry experiments. We hope that this blog post will ...

...GATC) Preparing unmethylated DNA, important when trying to cut with certain restriction enzymes (ex: ClaI...CCWGG) Preparing unmethylated DNA, important when trying to cut with certain restriction enzymes that are...

...entire series as it is published. For more reading try The Discipline of Teams by Jon R. Katzenbach and ...

...freezer. No more issues deciphering handwriting or trying to find to where the binder was moved. You’ll have...and you can’t remember where you put your flow cytometry protocol. Or maybe you can’t find the molecular...