...have been developed, which differ in the number, stability, and brightness of the fluorescent proteins used... (Cai et al., 2013), incorporates the highly photostable fluorophores mOrange2, EGFP, and mKate2 (red)...truncated, non-fluorescent form of YFP as the default state of the transgene such that recombination is necessary...not undergo recombination can be detected by immunostaining of the truncated YFP. In Thy1-Brainbow mice...2011) labels Drosophila neurons at the neuroblast stage, meaning that neurons of the same lineage will express...injecting a CMV-Brainbow construct at the embryonic stage. This technique could be used to target Brainbow...labeled by injecting Brainbow at the single-cell stage. Zebrabow, or “zebrafish brainbow,” is a collection...

...Recombinase-based state machines (RSMs), which take on a particular DNA-sequence configuration (state) based on...effects on the state cannot be undone and also limit the set of possible downstream states that can be achieved...order of inputs results in only one final state, then the state of the RSM will tell you precisely what ...might one read the state of an RSM? The most straightforward way to read the state of an RSM is to sequence...is to interrogate state with qPCR. The re-arrangement of DNA segments in each state of a register enables...certain states of interest. For example, in Figure 2 below we present a two-input, five-state GRSM register...green fluorescent proteins in each state. The three methods of state interrogation mentioned above (Sanger...

...In 2008 the Quake Lab at Stanford University became interested in exploring biological dark matter – ...Preparations ahead of time included contacting YNP staff to arrange permits to visit places not accessible...September we arrived in YNP just as the weather was starting to turn chilly at the 8,000 foot elevation. Due...environmental samples Since the high temperatures and unstable ground around these hot springs make them extremely...telescoping poles to collect water samples from a distance. At each collection site, we took GPS coordinates... collected small water and sediment samples, stabilizing them in ethanol and/or glycerol, since there ...universal conditions for culturing or long-term stabilization of unknown bacteria. Hiking all day and processing...

...example, I am not that interested in meeting movie stars, but when I met the astronaut Jim Lovell I was speechless...work one floor above mine in the lab of Dr. Jack Szostak, who went on to win the Nobel Prize. Dr. Doudna...whiteboard. The final image is pictured here…she started from a blank white slate and developed this over...paper in Science.  Interestingly, this project started with their labs studying the mechanisms of RNAi...

...entrepreneur must be OK with the constant fear of failure at all stages in the startup". To gain insight into "...team was unable to secure enough money to sustain a startup, he went to work for Flagship Ventures – a... his energy and effort into making the startup self-sustainable. Within a year from this decision, Mike...Development & Operations. Starting a biotech company When and how does a startup become "successful"? First... budding startup, I asked Mike to outline 3 pieces that are essential to the biotech startup puzzle: ...Assembly Standards Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)    ...occasionally pondered translating my research into a startup company. However, like many like-minded people,...

...into two stages: Dedifferentiation - Conversion of adult somatic cells into the pluripotent state. Redifferentiation...differentiated somatic cell reverts back to a pluripotent state, which then proliferates and is redifferentiated...lineage, without dedifferentiating into a pluripotent stage. Cellular reprogramming The reversal of a differentiated...differentiated cell type to an undifferentiated state and then redifferentiation into the cell type of choice...differentiated cells of choice. The dedifferentiation stage involves overexpression of four reprogramming factors...differentiated somatic cell to revert back to a pluripotent stage (iPSC formation) [2, 3]. The iPSCs then proliferate...reprogrammed to iPSCs successfully [10-12]. The next stage of reprogramming consists of redifferentiation of...

...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse the list...

...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene'...

...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene'...

...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene'...

...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene'...

...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene'...

...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene'...

...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene'...

...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic...

...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic...

...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic...

...AVEXIS palsmid set is all that you will need to start producing proteins for AVEXIS screening. It includes...

... Lab plasmids The Zinc Finger Consortium was established to promote continued research and development...

...Optogenetics The lab of Michael Davidson from Florida State University has contributed their comprehensive collection...