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We narrowed to 390 results for: promoter

Showing: 101 - 120 of 390 results

  1. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...extreme self-starters and especially good at self-promoting—No one is going to tell you to pick up the pace...diversity and acceptance can be an issue in getting promoted (especially race and gender) Interpersonal issues...

  4. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...limited by the lack of well characterized inducible promoters. Far removed from the bio-industry’s vision of... the same dCas9 protein. By using an inducible promoter to modulate dCas9 expression, multiple scRNA transcriptional...

  5. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...researchers test many different combinations of promoters, terminators, solubility and purification tags...2016). The variety of constitutive and inducible promoters, linkers, and tags makes EcoFlex a great option...been used to examine the effects of different promoters, terminators, enhancers, and cross-cassette interactions... et al., 2019). Many plasmid elements such as promoters or fluorophores are interchangeable across organisms...

  7. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ... vectors within this kit contain a variety of promoters to facilitate gene expression in bacteria, yeast...plasmids within it contain a variety of different promoters followed by leader sequences (encoding small leader...different genes was predictable based solely on the promoters and Shine-Delgarno sequences used in this so-called...

  8. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...protein whose coding sequence is closest to the promoter will be expressed. This system differs from Brainbow...translation-blocking STOP cassette immediately following the promoter. The STOP cassette includes a mutant YFP that .../FRT recombinase. When placed under different promoters, Brainbow and Flpbow can be used to label distinct...

  10. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...activated, this DREADD acts through the Gq pathway and promotes neuronal firing. Plasmid Serotype Name Depositor...more accurate cell and tissue targeting. While promoters and AAV serotypes can sometimes be used to target...

  12. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...efficient AAV prep for a 4.2 kB insert with the CMV promoter using the AAV-BR1 capsid? A: It is our understanding...4.7kB.  If your insert alone is 4.2 kB and CMV promoter+enhancer is 0.5 kB, then you would be oversized...

  13. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...light chain, each from a constitutively active CMV promoter. Constructs were then verified by both diagnostic... and heavy (blue) chains as driven by two CMV promoters (orange). Figure from Andrews, et. al., Elife  ...

  14. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...that flank the gene cassette, which contains a promoter, the gene of interest, and a polyA signal. Created...sequences are from AAV2 because they demonstrate high promoter activity (Earley et al. 2020), but non-AAV2 ITRs...

  16. Hot Plasmids - January 2023

    Type
    Blog Post
    ...depicted here: The heat-inducible Hsp (heatshock promoter) drives expression of the tumor-seeking CAR (chimeric...chimeric antigen receptor), whereas a ubiquitous promoter drives expression of an mCherry reporter.  After...

  17. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...search scoped to viral preps shows filters for promoter and serotype, among others. We also created a ...example, there is only one viral prep filter for promoter “Syn”, that, when selected, will match variations...

  19. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...suitability of various antibiotic resistance markers, promoters, and inducible gene expression systems in bee ...fusion is under control of the arabinose-inducible promoter. Cells are first induced with arabinose to begin...
Showing: 101 - 120 of 390 results