We narrowed to 610 results for: gan
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TypeBlog Post...asks for strong organizational skills. You might think, “I’d be good at that! I organized my lab data so...important part of the application. Even within an organization, there can be variance. At Addgene, our content...explain why you are interested in working for that organization or group. This is particularly important for...want to talk about! You could describe your organizational system, your rationale for designing it the...title of the position] at [name of the company/organization]. I am a [adjective that describes you] scientist...that particular job, your commitment to the organization's mission, or your deep interest in the lab's...
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Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog Post...Williams, Dave Baltrus, Geof Hannigan, Irene Newton, Juliet Johnston, Kat Milligan-Myhre, Kim Dill-McFarland...discussion with my dinner partners Denise Akob and Geof Hannigan that the outline for Binning Singletons came together...a positive stir and was recognized by meeting organizers in their town hall. Iterative improvement - ...Mentor-Singleton relationship. Some of the variability was organic, as some people need less assistance than others...take some leaps. Binning Singletons is its own organization, working to make scientific meetings more productive...Eric Griffis, Janet Iwasa, Shawnna Buttery, Kat Milligan-Myhre, and Pragati Chengappa. Financial and moral... -
CRISPR Plasmids - Purify Genomic Loci
TypeCollection...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR... -
CRISPR Plasmids - Xenopus
TypeCollection...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR... -
Fluorescent Protein Guide
TypeCollection...individual plasmids or protein-protein interactions in organs and whole mammals. Regulate Biological Activity... -
27 Hot Plasmids from 2016
TypeBlog Post...orphan receptors meaning their ligands are unknown. Identifying new ligands for orphan GPCRs is of significant...Caenorhabditis elegans CRISPR/Cas9 technology has revolutionized genome editing in multiple organisms thanks ...construction of a so-called “genomically recoded organism” (3) but its portability to other bacterial strains...monitoring and analysing autophagy of cytosol and organelles in yeast cells. Named after the brightly-coloured... both in vitro cell systems and diverse model organisms. Addgene’s newest TALEN kit, the FusX assembly...screens required to identify correctly modified organisms has limited routine use of this technology. To...CRISPR/Cas9-based genetic tag insertion in the C. elegans genome, Erik Jorgensen’s lab from the University... -
CRISPR Plasmids - Zebrafish
TypeCollection...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR... -
CRISPR Plasmids - Parasites
TypeCollection...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR... -
CRISPR Plasmids - CRISPR Transposases (CAST)
TypeCollection...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR... -
Fluorescent Protein Guide: In Vivo Imaging
TypeCollection... or protein-protein interactions in deep-tissue organs and whole mammals. Genetically encoded fluorescent... -
Structural Genomics Consortium Plasmids
TypeCollection...Structural Genomics Consortium is a not-for-profit organization that aims to determine the three dimensional... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post... (XFP) of different colors to label an organ or entire organism. Because each cell can have multiple copies...The ability to track single cell dynamics at the organism level has been made possible by tools that allow.... Genes encoding the fluorescent proteins are organized in tandem with a single promoter, but only the...developed for tracking various cell types and organisms and for improving the level of detail possible...that allow for multicolor labeling of an entire organism or a specific tissue. XFPs are introduced using...systems allow single cell dynamics in a variety of organisms to be observed in more detail than ever before... -
Plasmids 101: Biotinylation
TypeBlog Post...an essential coenzyme that is required by all organisms for life. Biotin is only synthesized by plants...enzymes catalyze the transfer of carboxyl groups to organic acids to produce important cellular metabolites...post-translational protein modification. Most organisms have less than five enzymes in the entire proteome...for biotinylating only one lysine in the entire organism on the biotin carboxyl carrier protein (BCCP)....them is one of the strongest noncovalent protein-ligand interactions found in nature. Once the bond is ...is unaffected by changes in pH, temperature or organic solvents. Uses of biotin in molecular biology Biotin...these features it isn’t surprising that scientists began to exploit biotin and its strong interaction with... -
Plasmid Collections
TypeCollection...Collections A modular set of plasmids from a depositor organized by a common use. Click here to view all collections... -
CRISPR Plasmids - gRNAs
TypeCollection...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR... -
CRISPR Plasmids - Cascade-Cas3
TypeCollection...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR... -
TALEN Engineering
TypeCollection...yielded TALENs which function efficiently in: C. elegans ( Wood et al., Science 2011 ) Zebrafish ( Sander... -
Viral Vectors 101: Optogenetic Tools
TypeBlog Post...tethered ligands hold the ligand in close proximity to the receptor, when exposed to light, the ligand engages...photoswitchable tethered ligands (PTL), and photoswitchable, orthogonal, remotely-tethered ligands (PORTLs). OptoXRs... them. PORTLs use a similar concept, except the ligand is attached to a protein binding partner or nanobody..., McKnight, D. J., Yoshizawa, S., Kato, H. E., Ganguli, S., & Deisseroth, K. (2019). Cortical layer–specific... -
Understanding GPCR Signaling Using TRUPATH
TypeBlog Post...transducer complexes that are activated in response to a ligand binding a GPCR of interest. How does TRUPATH work...intracellular surface of the GPCR. Binding of a ligand to the extracellular surface of the GPCR causes...coelenterazine, followed by the addition of the endogenous ligand or drug. In the inactive state, the luminescent...emission. Following activation in response to the ligand, the complex will disassociate, resulting in a ...across the transducerome for a specific receptor-ligand pair. They further used TRUPATH to analyze a panel...This collection can be used to study endogenous ligands, the pathways they activate, as well as the activation... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog Post...designer ligands that can be simply introduced by injection. The main challenge in using ligands is ensuring...at broad tissue or system-wide targeting, as the ligand can reach target cells throughout the body. Controlling...chemogenetics, while different concentrations of ligands can be injected, this is not as precise and the...brain. One way to address this is by working with organisms with light accessible brains, such as zebrafish...while also being selective and effective. A new ligand from the Bryan Roth lab and Takafumi Minamimoto...