...to mid-sized companies and organizations. Addgene as a nonprofit organization is very considerate and thoughtful... outside an organization The next step is to expand your efforts beyond your organization and/or scientific...people from various organizations in science and beyond. For example, I help organize scientific workshops...guide: Collaborating with others outside your organization also known as external collaborations. Interestingly...the ability to work with others outside one’s organization was found in the aforementioned survey as one... partnerships where we can leverage another organization’s expertise to our mutual benefit.  Our partnership...small: Collaboration opportunities within an organization but outside your comfort zone As part of my ...

...with low affinity for ligands. When integrins interact with extracellular ligands, their ectodomains can... binding ligands with high affinity. This extended-open state is key to strengthening ligand binding, ...almost all bind outside the ligand-binding pocket, their sheer size impeding ligand attachment. The five other...often express multiple integrins that bind the same ligand, making it difficult to dissect the function of... antibodies in the collection are able to block ligand binding — and therefore interrupt integrin function...ascertain which integrins are responsible for specific ligand-binding functions, all key to basic biomedical ...intracellular ends and large ectodomains containing ligand-binding heads and long legs that protrude from ...

...RNAi in a wide range of cell types, tissues and organisms Luk Parijs 11619 pLB Modified pLL3.7; Genetic ...

...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...

...www.taleffectors.com) . Whether you work in a C. elegans lab and have been struggling to mutate a specific...

...variety of recombinase systems. Picking Ligands Picking the right ligand is also important for building a chemogenetic...reduced off target activation by ligands. PSAMs themselves are modified ligand binding domains. When coupled... Peripheral Nerves Produces Ligand-Dependent Analgesia, as well as Ligand-Independent Functional Changes...a method for targeting receptor expression as a ligand and receptor pair. Targeting Receptor Expression...expressed in the brain, it is necessary to use ligands capable of crossing the blood-brain barrier.  Another...  Another consideration when picking ligands for chemogenetic studies is whether or not your study is aimed...reprogrammed to respond only to inert, nonnative ligands. However, they retain their intracellular interactions...

...genetic code is used by most organisms on Earth, but different organisms have different preferences for...codons are used preferentially in which organisms - organisms have codon usage bias. You can find many...peptide of interest and produce that peptide in your organism of choice. Unfortunately, codon preferences make...and expect your sequence to express well in any organism. So what are the evolutionary constraints that... do about them? Read on to find out!   Why do organisms have different codon usage biases? The reasons...reasons for varied codon preferences among organisms aren’t completely understood, but some possible reasons... tRNAs with the appropriate amino acids. If an organism uses only a subset of codons, it only needs to...

... CRISPR-based genome editing in organoids By Ashley Waldron Organoids are three-dimensional cultures that...toolkit for performing genetics studies in organoids. The Organoid EasyTag System is a straightforward workflow...in an organoid model (Sun, et al. 2021).  Figure 1: Example schematic from the Organoid EasyTag...fetal-lung derived organoid system, the approach is intended to be amenable to organoids derived from other...find in this article: CRISPR-based editing in organoid Biosensors for detecting strigolactones The CRISPR... that mimic aspects of organ morphology and physiology in vitro and have emerged as a powerful model for...disease. In order to expand their model’s impact, organoid users have recently begun to develop CRISPR-based...

...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...

... not required to be fluorescent. A variety of organic dyes and fluorescent proteins have been developed...

... this genetic material to specific cells in an organism. Figure 1. Using viral plasmids to generate virus...

...The ligands that activate many GPCRs are still unknown. To identify some of these unknown ligands and ...site-directed mutagenesis to bind non natural ligands. These non natural ligands however exhibited off-target effects...receptors can be activated by an array of different ligands, including hormones, neurotransmitters, photons...membrane. The receptor recognizes the appropriate ligand to activate the G proteins. It is estimated that...signaling works GPCR signaling is initiated when a ligand binds to the extracellular surface of the GPCR....cascades as a response to the extracellular stimuli. Ligand dissociation from the GPCR allows for binding of...scientists decipher the signaling pathways from ligand to receptor to G-protein, and finally, to a response...

...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...

...reagents to use - organic solvents and detergents.  Organic Solvents  As mentioned above, organic solvents can... for use with this type of fixation*. Organic Solvents Organic solvents, generally acetone, methanol,...types of fixatives - chemical cross-linkers and organic solvents.  Chemical Cross-Linkers Chemical cross-linkers...quite as well as a cross-linker. This is because organic solvents can be quite harsh - lipids and soluble...disrupt your target epitope (Fig. 1). So, while organic solvents may improve antibody binding for some ...targets, they can destroy it for others. That said, organic solvents’ impact on lipids means that they are ...and permeabilize a sample. But you can also use organic solvents after fixing your sample with a cross-...

...different organisms. There are several codons that encode the same amino acid. Each organism, however,...the genetic code is universal, many different organisms actually prefer certain codons over others for... biologist who wants to express genes from one organism in another? Let’s take a look at codon usage and...optimize codons for expression in a particular organism. Why is codon usage important? Translation and...for codon-usage of 257,468 genes across 8,792 organisms (Nakamura et al., 2000).  What is codon optimization...expression by changing synonymous codons based on an organism's codon bias. The idea is that scientists can make...mutations throughout a gene of interest based on an organisms codon usage bias to increase translational efficiency...

...and study certain behavioral responses in model organisms like mice, zebrafish, and Drosophila. These tools...LAD Light-activated dimerization, using proteins GIGANTEA (GI) and the light oxygen voltage (LOV) domain...engineered zinc finger proteins, using proteins GIGANTEA (GI) and the light oxygen voltage (LOV) domain...

...institutions.  What is the UBMTA, you ask? Well, organizations used to negotiate individual MTAs every time...standardized terms makes it a lot easier for organizations to make policy decisions on research use agreements...2023, we facilitated 784,795 MTAs with 8,798 organizations in 111 countries. From 2019 to 2023, we averaged...Addgene parlance, the depositor and requestor) organizations. Over the last twenty years, we’ve developed...When you deposit a plasmid with Addgene, your organization’s tech transfer office is sent a deposit agreement...distributed. In signing this agreement, your organization agrees to let Addgene receive, store, and distribute...individual MTAs for each request, the depositing organization signs one form that can be used for every MTA...

... development. These are required for a growing organism to make the complicated transition from a clump...dynamic processes are largely absent in adult organisms, where tissues structures are more stable and ...movement from the bone marrow and other lymphoid organs to specific tissues where they can scan for any...is large scale cellular movement throughout an organism. Studying cell mobility within these complex...tools to directly follow immune cells in lymphoid organs. The development of cell sorters together with ...antibodies made it possible to track cells from one organ to another. Thus several labs were able to decipher...microscopy images of chemically coloured lymphoid organs did in the past. How do researchers track fluorescently...

...their physiological endocrine ligands. Nuclear receptors and their ligands NRs represent the largest family...depending upon a context defined by ligand, cell type, and promoter, organizing the expression of functional ...McKenna and Bert W. O’Malley. Survival of all organisms depends on efficient energy maintenance - through...nervous system signaling to peripheral effector organs, which then return information such as nutritional...processes are regulated at the cellular, tissue and organ level by nuclear receptors (NRs), their coregulators... of such signals between central and peripheral organs because of their established roles as molecular...stems in part from their ability to function as ligand-dependent sensors for a diverse set of fat-soluble...

...Chalfie expressed GFP in exogenous organisms (E. coli, and later C. elegans) for the first time. After Chalfie...proteins could be responsible and began studying these marine organisms to find the culprits. He even obtained...These molecules occur naturally in bioluminescent organisms, but their real power lies in the clever ways ...Although humans have known about bioluminescent organisms and their characteristic radiance for some time...to isolate two proteins from the jellyfish’s photoorgans; the first, which he called aequorin, gives off...localization. And as it turns out, he was right! He began to study the A. victoria gene responsible for encoding...scientists really started to take notice of GFP, and began to realize its potential as a research tool. Soon...