Even more elegant: Single injection CRISPR/Cas9 in C. elegans
Type
Blog Post
Published
July 7, 2015, 3:36 p.m.
...engineering methods for C. elegans were released, signaling a new era in C. elegans research. Homology directed... CRISPR/Cas9 editing in C. elegans:
Engineering the Caenorhabditis elegans genome using Cas9-triggered...custom genomic modifications, is very robust in C. elegans, and the methods for HDR-mediated modification ...University of North Carolina has made CRISPR in C. elegans even easier - now, one can generate a fluorescent... gene tagging approach
Multiple features of C. elegans have made CRISPR/Cas9 so successful in this species...used a transgene selection strategy for their C. elegans/CRISPR experiments, but this strategy required ...it possible fluorescently label proteins in C. elegans, they inserted the SEC into a synthetic intron ...