...implications of using these technologies, but also the rapidity of the technologies’ adoption by the scientific...amongst the Nobel prize winners for developing the technology. Though these case studies are “old,” they lead... learning addresses the dilemma of creating a technology that could eventually replace scientists themselves...Addgene’s role in the rapid dissemination of these technologies, proposing that “Addgene, with its easy, fast...CRISPR-Cas plasmids, has been a key driver of the technology.” As an Addgene vet of 12 years, I’m very familiar...decline in the competitiveness of U.S. science and technology.” This last section of the book, Future Science...

...in the malaria community for the better (Nat. Biotechnol., 2014). Given the timing, it shouldn’t be a ...yet. As a genome editing tool, the CRISPR/Cas9 technology has been surrounded by a whirlwind of research... sgRNAcas9. Top tools for transferring CRISPR technology to a new organism: For obvious reasons you can...developed with the purpose of applying CRISPR technologies to new organisms and will help you whether you...Report your findings to help everyone else. CRISPR technology has reached into so many different communities...falciparum using the CRISPR-Cas9 system." Nature biotechnology(2014). PubMed PMID: 24880488. 2. Hsu, Patrick...specificity of RNA-guided Cas9 nucleases." Nature biotechnology 31.9 (2013): 827-832. PubMed PMID: 23873081....

...contributed by Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans Health Administration...roles and my current work as a AAAS Science and Technology Policy Fellow (STPF) at the Veterans Health Administration...these resources compiled by the AAAS Science and Technology Policy Fellowship Program to learn more about...could understand how to make new treatments and technologies accessible to patients. And what better place...guest blogger Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans Health Administration... Guerra, PhD, is currently a AAAS Science and Technology Policy Fellow at the Veterans Health Administration...particularly interested in driving scientific and technological innovation that is accessible and equitable ...

...CRISPR-Cas nucleases in human cells." Nature biotechnology 31.9 (2013): 822-826. PubMed PMID: 23792628....on-and off-target genome editing." Trends in biotechnology 33.2 (2015): 132-140. PubMed PMID: 25595557....RNA-programmed Cas9 nuclease specificity." Nature biotechnology 31.9 (2013): 839-843. PubMed PMID: 23934178....off-target cleavage by CRISPR-Cas nucleases." Nature biotechnology 33.2 (2015): 187-197. PubMed PMID: 25513782....of zinc-finger nuclease specificity." Nature biotechnology 29.9 (2011): 816-823. PubMed PMID: 21822255....integrase-defective lentiviral vectors." Nature biotechnology 33.2 (2015): 175-178. PubMed PMID: 25599175....breaks induced by engineered nucleases." Nature biotechnology 33.2 (2015): 179-186. PubMed PMID: 25503383....

... of Rogue Bioethics. On November 25, the MIT Technology Review dropped a bombshell report. A scientist... is unethical to use scientifically unproven technologies to edit a human embryo, or have a woman carry...controversial and ethically fraught rapidly emerging technology. We could do this for CRISPR/Cas9 – or any other...germline editing, we will initially limit the technology to research centers engaged in long-term, mandatory...review and decide on the future of genome editing technology. We must stop punting decisions on scientific... inform her work on emerging issues in novel technologies, including self-driving vehicles, synthetic ...

...Gram Negative. Technology Networks (2019) https://www.technologynetworks.com/immunology/articles/gram-...

...treatment can lyse cells. Alcohols, like ethanol or methanol, fix and permeabilize in a single step by...sample (1). When combined with cell sorters, this technology can be used to isolate a specific subset of cells...and further analyze that subset of cells. An immunologist, for example, might be interested in the lymphocytes...cell into distinct populations (Figure 2). An immunologist who studies B cells, might then gate the B cell... Cytometry: An Overview. Current Protocols in Immunology 120: . https://doi.org/10.1002/cpim.40 Picot ...retrospective, fundamentals and recent instrumentation. Cytotechnology 64:109–130 . https://doi.org/10.1007/s10616...

...you want to read more content related to CRISPR technology, subscribe to our CRISPR blog content.   In this... dCas12f to VP64. Read the paper in Nature Biotechnology Find the Cas12f-GE plasmids Programmed genome...of target genes and cell types. Their Nature Biotechnology paper showcases an improved design of the guide...editing efficiency. Read the paper in Nature Biotechnology Find the epegRNA plasmids Enhanced prime editing... paired pegRNAs.  Read the paper in Nature Biotechnology Find the PRIME-del plasmids  Deletion and replacement...expression in the liver.  Read the paper in Nature Biotechnology Find the PEDAR plasmids   Additional resources...

...rapidly decode the complexity? The CombiGEM-CRISPR technology was developed with the goal of providing an easy-to-use...This blog post will introduce you to this new technology, and guide you through the basics of CombiGEM-CRISPR...particularly interested in developing and applying new technologies to interrogate and understand complex biological... off-target effects of CRISPR-Cas9." Nature biotechnology (2016). PubMed PMID: 26780180. PubMed Central...lentiviral CRISPR-guide RNA library." Nature biotechnology 32.3 (2014): 267-273. PubMed PMID: 24535568....combinatorial genetics in human cells." Nature biotechnology (2015). PubMed PMID: 26280411. PubMed Central...

...about Mammalian shRNA Tools for RNAi! Cre-Lox technology CAG-GFP-IRES-CRE (Plasmid #48201) This retroviral... and Cre-recombinase. The Cre-lox system is a technology that can be used to induce site-specific recombination...knockout, and selection marker removal. CRISPR technology pdCas9-humanized (Plasmid #44246) Expression ... the pLKO.1 vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408)   This...lentiviral prep from the Addgene viral service. CRISPR technology lentiCRISPR v2 (Plasmid #52961) This is a 3rd...Maetzig T, et al. Gammaretroviral Vectors: Biology, Technology and Application. Viruses. 2011. PubMed PMID: ...

...University in Lisbon, Portugal. She leads the Glycoimmunology group based at the Research Unit on Applied...reported that PMM2-CDG patients suffer from immunological defects. These are characterized by a predisposition...play an essential role in almost every aspect of immunology, participating in events such as cell development... J., dos Reis Ferreira, V. & Videira, P. A. Immunological aspects of congenital disorders of glycosylation...winners! Paula Videira is the team leader of the Glycoimmunology group at UCIBIO, FCT-UNL, Portugal. Her main...role of glycans. Follow her on Facebook: @glycoimmunologygroup.     Michael McMurray is an Associate Professor...

... hood with a disinfectant (most people use 70% ethanol, but you can also use Incidin wipes) before and...everything that’ll be brought under the hood with 70% ethanol, including your glove-covered hands! You should...spills do occur, they should be wiped with 70% ethanol immediately. Finally, avoid sticking pipettes into...Most cell culture media contain the pH indicator phenol red (pH range 6.0 (yellow) - 8.0 (red)). Blood ...is particularly interested in Glycobiology and Immunology. You can find Sana on LinkedIn.   References ...et al. "Gene expression profiling analysis of bisphenol A-induced perturbation in biological processes...

... covers a brief review of different basic BE technologies and their adaptation for plant genome editing...State University Kutubuddin Molla is an Ag-biotechnologist in the National Rice Research Institute, Cuttack...CRISPR-Cas9 cytidine deaminase fusion." Nature biotechnology 35.5 (2017): 441. PubMed PMID: 28346401. 6. ...with a Cas9-cytidine deaminase fusion." Nature biotechnology 35.5 (2017): 438. PubMed PMID: 28244994. 7. ...fusion of nCas9 and human APOBEC3A." Nature biotechnology (2018). PubMed PMID: 30272679. 8. Hua, Kai, ...scope in rice by using Cas9 variants." Plant biotechnology journal (2018). PubMed PMID: 30051586. 9. Hua...

...group developed the original iteration of this technology in 2007. Used to label neurons in the cerebellum...cerebellum of mice, the team appropriately named their technology Brainbow. Brainbow 1.0 used the fluorophores ... promoter. Zebrabow is an extremely versatile technology as recombination can be induced in developing...cells. Tetbow optimizes Brainbow for current technologies Tetbow (Sakaguchi et al., 2018) overcame the...multiple fields of view, whereas previous Brainbow technologies were useful for discriminating neurons within... continue to develop new iterations of these technologies, expect to see systems that use newer, more ...

...CRISPR and epigenetics As with many TALEN-based technologies, the advent of CRISPR has made targeting much...modification is certainly not the only CRISPR-based technology designed to alter gene expression. Fusing dCas9... genes from promoters and enhancers. Nature Biotechnology 33:510–517 . https://doi.org/10.1038/nbt.3199...regulatory elements in the human genome. Nature Biotechnology 35:561–568 . https://doi.org/10.1038/nbt.3853...programmable TALE-TET1 fusion proteins. Nature Biotechnology 31:1137–1142 . https://doi.org/10.1038/nbt.2726...modifications at endogenous enhancers. Nature Biotechnology 31:1133–1136 . https://doi.org/10.1038/nbt.2701...

...hours of culture, when glucose is abundant and ethanol concentrations are low, pADH1 displays low promoter...activity of the strong yeast promoter pTEF). As ethanol accumulates, it binds to the repressor, enabling...engineered pADH1 variants that are less sensitive to ethanol, including the “middle” ADH1 promoter pADH1m, which... which maintains activity during the late ethanol consumption phase of yeast culture. Repressible Binary... regulated expression in mammalian cells. BMC Biotechnol 6, 43 (2006). https://doi.org/10.1186/1472-6750...expression system for Bacillus. Appl Microbiol Biotechnol. 2019 Jan;103(1):303-313. doi: 10.1007/s00253...

...wasn’t that easy. In the 1980s, gene targeting technology was invented to introduce specific changes into...into the mouse genome. With these early technologies, a researcher would first introduce a mutation into...transgenic offspring. Although powerful, this technology is cumbersome to use, not that efficient, often...first exposure to CRISPR when he used this new technology to engineer mouse models (Wang et al., 2013, ...others around the world. Yes, finally, we have a technology, CRISPR, that is simple in concept, straightforward...for CRISPR-Cas9–mediated gene inactivation. Nat Biotechnol 32:1262–1267 . https://doi.org/10.1038/nbt.3026...

...University of Science and Technology in Shijiazhuang, defended the technology in a news article in Nature..., NgAgo. As you might expect with any nascent technology, researchers are having trouble getting NgAgo...in this informative post. Interestingly, this technology may make some uses of Site Directed Mutagenesis...scientists. You can find all of our August posts in chronological order here: Site Directed Mutagenesis by PCR...

...who recently received a Ph.D. in Biology and Biotechnology from Worcester Polytechnic Institute. What is...With the meteoric rise and diversity of CRISPR technologies, one might incorrectly relegate RNAi to history...requires the presence of dsRNA. In plants, CRISPR technologies are predominantly used to disrupt function at... throughput survival screen found that either technology was equivalently precise, but each method recovered...Orr recently earned his Ph.D. in Biology and Biotechnology from Worcester Polytechnic Institute where he...Cas9 and RNAi screens for essential genes. Nat Biotechnol 34:634–636 . https://doi.org/10.1038/nbt.3567...

...’re trying to make MTA turnaround faster with technology transfer offices (TTOs). The latest of these ...University  Broad Institute  California Institute of Technology Cold Spring Harbor Laboratory  Dana-Farber Cancer...from Addgene MTA: material transfer agreement Technology Transfer: the exchange of intellectual property...universities and other research institutions TTO: Technology Transfer Office. Some synonymous terms include...Addgene.org Addgene Help Center: Tech Transfer Technology Transfer Information Tech Transfer News: A Reintroduction...