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Showing: 101 - 120 of 772 results

  2. Plant Plasmids and Resources

    Type
    Collection
    ...editing across crop species and genotypes - Zhenghe Li. A robust CRISPR/Cas9 system for convenient, high-efficiency... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help...

  3. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Transcription Factor Knockout Library 162275 Knockout Human Li 3rd ~7 11,364 Dow Lab Base Editing Sensor Libraries...UBDUB CRISPR Knockout Library 171531 Knockout Human Li 3rd ~6 9,274 MYC-CRISPR Library 173195 Knockout Human...different properties to create a custom filtered list of the CRISPR pooled libraries in Addgene's collection...Human Vakoc 3rd 6 3,051 Human Lipid Droplet and Metabolism Library 191535 Knockout Human Olzmann 3rd ~10...Yusa 3rd 5 90,230 Mouse Lipid and Sphingolipid Metabolism Knockout Libraries 206248 206249 Knockout Mouse...Inhibition Yeast Tavazoie N/A 6-12 51,681 Mouse Metabolism Library 163966 Knockout Mouse Kimmelman 3rd ~...

  4. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ..., Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS, Huang...Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, Gonzales AP, Li Z, Peterson RT, Yeh JR, Aryee MJ, Joung JK. 2015....mammalian cell culture. Five years later, PubMed lists more than 6,300 CRISPR-related publications, many...

  6. Zhang Lab CRISPR Page

    Type
    Collection
    ...DA, Weinstein JA, Ran FA, Konermann S, Agarwala V, Li Y, Fine EJ, Wu X, Shalem O, Cradick TJ, Marraffini...CRISPR-Cas9. Swiech L, Heidenreich 1, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol . ... of Cas9 in vivo and ex vivo , the Zhang Lab established Cre-dependent and constitutively expressing Cas9...

  7. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ... . https://doi.org/10.1038/mt.2009.280 Hirsch ML, Li C, Bellon I, Yin C, Chavala S, Pryadkina M, Richard...Iodice C, Polishchuk EV, Sommella A, Colecchi L, Rossi S, Simonelli F, Giunti M, Bacci ML, Polishchuk RS, Auricchio...Marrocco E, Rossi S, Giunti M, Palfi A, Farrar GJ, Polishchuk R, Auricchio A (2013) Effective delivery of large...

  8. Antibodies 101: Validation

    Type
    Blog Post
    ...Cordell JL, Cragg MS, Šerbec VČ, Jones M, Lisnic VJ, Kremer L, Li D, Koch-Nolte F, Pascual N, Rodríguez-Barbosa...unless you share the results with others. If you publish data generated using your antibody, it’s best scientific...

  9. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    .... Biol., Apr. 2016. PubMed PMID: 27075976. 4.  X. Li, E. R. Burnight, A. L. Cooney, N. Malani, T. Brady...enhanced random integration efficiencies that are accomplished with transfections and are therefore virus-free...enhance gene editing? Gene editing can now be accomplished in a more cost-effective and efficient manner...team.     Mathew Goodwin is a Technical Sales Specialist at Transposagen. As a gene editing enthusiast...Singh, V. V Adjan Steffey, T. Yeshi, and D. W. Allison, “Gene Editing in Human Pluripotent Stem Cells:... Perry, V. V. A. Steffey, K. Miller, and D. W. Allison, “Decoding the Epigenetic Heterogeneity of Human...

  10. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...science.aae0169 2. Long C, Amoasii L, Mireault AA, McAnally JR, Li H, Sanchez-Ortiz E, Bhattacharyya S, Shelton JM, ... treat disease postnatally. Now, three papers published concurrently in Science have shown CRISPR can ...

  11. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...22205518. PubMed Central PMCID: PMC3574576. Lai, Sen-Li & Tzumin Lee. “Genetic mosaic with dual binary transcription...multiple genes at the same time.  The 3 binary systems listed above are orthogonal, meaning that they do not ...doi.org/10.1038/s41598-022-07852-7 De Boer, Herman A., Lisa K. Comstock & Mark Vasser. “The tac promoter: a ...

  15. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...plasmids from this paper at Addgene. 6. Xing, Hui-Li, et al. “A CRISPR/Cas9 toolkit for multiplex genome...Originally published Jan 28, 2016 and last updated Sep 10, 2020 by Jennifer Tsang. CRISPR makes it easy...

  16. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...phosphoserine resource for phosphoproteomics. Gassaway BM, Li J, Rad R, Mintseris J, Mohler K, Levy T, Aguiar M... and a new pSerOTS. Recently the Rinehart lab published a new strain of recoded E.coli (rEcoli XpS 192872...

  17. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...PDB ID 7S4X). The end of the DNA:RNA heteroduplex lies between the RuvC (orange) and HNH (red) domains,... will destabilize the nascent heteroduplex and abolish cleavage (Pattanayak et al., 2013). As mentioned...

  18. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...Wilson, Ning Sun, Deepak M. Gupta, Mei Huang, Zongjin Li, Nicholas J. Panetta, Zhi Ying Chen, Robert C. Robbins...reported in González et al. A percentage is not listed because many variables (cell type, criteria used...Check out the links at the end of the post for a list of Tet-inducible reprogramming vectors available...

  19. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Zhao F, Luo MH, Gong L, He M, Zhou P, Paninski L, Li B. 2017. The central amygdala controls learning in...

  20. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...Primers for reference). Many sequencing cores have a list of common primers that can be requested for no additional...used by Addgene during quality control? Addgene lists the primers used to obtain each result above the...
Showing: 101 - 120 of 772 results