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Showing: 1 - 20 of 125 results

  1. Biosensor AAV Preps

    Type
    Collection
    ...Constitutive 9 Li 113050 pAAV-hSyn-GRAB_DA1h Syn GRAB_DA1h none Constitutive 9, rg* Li 140553 pAAV-hsyn-GRAB_DA2m...none Constitutive 9 Li 140554 pAAV-hsyn-GRAB_DA2h Syn GRAB_DA2h none Constitutive 9 Li 140555 pAAV-hsyn-...none Constitutive 9 Li 140556 pAAV-hsyn-GRAB_rDA1m Syn GRAB_rDA1m none Constitutive 9 Li 140557 pAAV-hsyn-GRAB_rDA1h...Constitutive 9 Li 140558 pAAV-hsyn-GRAB_rDA-mut Syn GRAB_rDA-mut (control) none Constitutive 9 Li 208698 pAAV-hSyn-GRAB-gDA3m...Constitutive 9, rg* Li 123309 pAAV-hSyn-GRAB_NE1h Syn GRAB_NE1h none Constitutive 9, rg* Li 123310 pAAV-hSyn-GRAB_NEmut...Constitutive 1, 9 Li 185388 pAAV-hSyn-GRAB_OTmut Syn GRAB_OTmut (control) none Constitutive 1, 9 Li Voltage Reporter...pAAV-hSyn-GRAB_ACh3.0 Syn GRAB_ACh3.0 none Constitutive 9 Li Calcium Sensor: GCaMP8f 162376 pGP-AAV-syn-jGCaMP8f-WPRE...

  2. Brain Initiative Collection

    Type
    Collection
    ...fluorescent dopamine(DA) sensor GRAB_DA1m in neurons Yulong Li 113050-AAV9 pAAV-hSyn-GRAB_DA1h express the genetically-encoded...fluorescent dopamine(DA) sensor GRAB_DA1h in neurons Yulong Li 113050-AAVrg pAAV-hSyn-GRAB_DA1h express the genetically-encoded...fluorescent dopamine(DA) sensor GRAB_DA1h in neurons Yulong Li 115892-AAV8 pAAV-Syn-Archon1-KGC-GFP-ER2 AAV-mediated...norepinephrine(NE) sensor GRAB_NE1m in neurons Yulong Li 123308-AAVrg pAAV-hSyn-GRAB_NE1m Expresses the genetically-encoded...norepinephrine(NE) sensor GRAB_NE1m in neurons Yulong Li 123309-AAV9 pAAV-hSyn-GRAB_NE1h Expresses the genetically-encoded...norepinephrine(NE) sensor GRAB_NE1h in neurons Yulong Li 123309-AAVrg pAAV-hSyn-GRAB_NE1h Expresses the genetically-encoded...norepinephrine(NE) sensor GRAB_NE1h in neurons Yulong Li 123310-AAV9 pAAV-hSyn-GRAB_NEmut Expresses the genetically-encoded...

  3. Synthetic Biology - Metabolism

    Type
    Collection
    ... Collections Synthetic Biology Metabolism Synthetic Biology: Metabolism SynBio Resources SynBio Home Browse...Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling...Natural Product Pathways Efflux Pumps Flux Control Metabolism Plasmids Search the table by keyword or sort ... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help...

  4. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ..., (Constitutive or Cre-dependent) Li Lab GRAB_Ado Plasmids Yulong Li ADP A fluorescent, reagentless biosensor.... 2021 Dec 17. pii: S0896-6273(21)00988-0. Yulong Li ATP/ADP Monitor the ATP:ADP ratio during live-cell...studies. Nat Biotechnol. 2018 Sep;36(8):726-737. Yulong Li Acetylcholine Optimized acetylcholine sensor for ...2020 Sep 28. pii: 10.1038/s41592-020-0953-2. Yulong Li Acetylcholine Fluorescent sensor for in vivo acetylcholine...activity in vivo. Nat Methods. 2023 Nov 30. Yulong Li Dopamine Dopamine sensors GRAB_DA2 Next-generation...vivo. Nat Methods. 2020 Nov;17(11):1156-1166. Yulong Li Dopamine Ultrafast neuronal imaging of dopamine dynamics...2021 Nov 11. pii: 10.1038/s41587-021-01074-4. Yulong Li GABA GABA Sensor (iGABASnFR) (Constitutive or Cre-dependent...

  5. Retrograde AAV viral preps

    Type
    Collection
    ...fluorescent dopamine(DA) sensor GRAB_DA1h Dopamine sensor Li 123308 pAAV-hSyn-GRAB_NE1m Syn genetically-encoded...norepinephrine(NE) sensor GRAB_NE1m Norepinephrine sensor Li 123309 pAAV-hSyn-GRAB_NE1h Syn genetically-encoded...norepinephrine(NE) sensor GRAB_NE1h Norepinephrine sensor Li 123310 pAAV-hSyn-GRAB_NEmut Syn GRAB-NEmut control...sensor (does not respond to NE) Norepinephrine sensor Li 20297 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA...retrograde AAV based on the functional catagories listed below. Narrow down the items available within a...

  6. p53 Pathway

    Type
    Collection
    ...of tumors in early adulthood, a disorder known as Li-Fraumeni syndrome. Recently some impacts of p53 mutations...PubMed PMID: 19776742 . Germline TP53 mutations and Li-Fraumeni syndrome. Varley JM. Hum Mutat. 2003 Mar...pathway Background p53 Pathway Plasmids p53 Gene List References Background p53 is a transcription factor...transcriptional co-activators and modulate transcription. The list of p53 transcriptional targets is ever-expanding...available plasmids for the gene, or browse the gene list below. For multiple isoforms or subunits, individual...with the help of . Return to top p53 Pathway - Gene List Click on a name to find available plasmids for the... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help...

  7. CRISPR Guide

    Type
    Collection
    ...Bartolomei, M. S., Ferguson-Smith, A., Li, D., Yang, L., Wu, Y., & Li, J. (2015). CRISPR-Cas9-Mediated Genetic...: 25494202 Liu, X., Zhang, Y., Chen, Y., Li, M., Zhou, F., Li, K., Cao, H., Ni, M., Liu, Y., Gu, Z., Dickerson...: 23287718 Cui, Z., Tian, R., Huang, Z., Jin, Z., Li, L., Liu, J., Huang, Z., Xie, H., Liu, D., Mo, H...., J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., Cradick, T....V., Nguyen, N. T., Zheng, Z., Gonzales, A. P. W., Li, Z., Peterson, R. T., Yeh, J. J., Aryee, M. J., &...29160308 Hess, G. T., Frésard, L., Han, K., Lee, C. H., Li, A., Cimprich, K. A., Montgomery, S. B., & Bassik...., Levesque, S., Jing, Y., Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt, R., Solley, S. ...

  8. mTOR Pathway

    Type
    Collection
    ...PMID: 22500797 . Rapamycin: one drug, many effects. Li J, Kim SG, Blenis J. Cell Metab. 2014 Mar 4;19(3)...Screening Kit Background mTOR Pathway Plasmids mTOR Gene List Resources The m echanistic or m ammalian t arget...available plasmids for the gene, or browse the gene list below. For multiple isoforms or subunits, individual...David Sabatini . Return to top mTOR Pathway - Gene List Click on a name to find available plasmids for the... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help...

  9. Plant Plasmids and Resources

    Type
    Collection
    ...editing across crop species and genotypes - Zhenghe Li. A robust CRISPR/Cas9 system for convenient, high-efficiency... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help...

  10. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Transcription Factor Knockout Library 162275 Knockout Human Li 3rd ~7 11,364 Dow Lab Base Editing Sensor Libraries...UBDUB CRISPR Knockout Library 171531 Knockout Human Li 3rd ~6 9,274 MYC-CRISPR Library 173195 Knockout Human...different properties to create a custom filtered list of the CRISPR pooled libraries in Addgene's collection...Human Vakoc 3rd 6 3,051 Human Lipid Droplet and Metabolism Library 191535 Knockout Human Olzmann 3rd ~10...Yusa 3rd 5 90,230 Mouse Lipid and Sphingolipid Metabolism Knockout Libraries 206248 206249 Knockout Mouse...Inhibition Yeast Tavazoie N/A 6-12 51,681 Mouse Metabolism Library 163966 Knockout Mouse Kimmelman 3rd ~...

  11. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ..., Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS, Huang...Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, Gonzales AP, Li Z, Peterson RT, Yeh JR, Aryee MJ, Joung JK. 2015....mammalian cell culture. Five years later, PubMed lists more than 6,300 CRISPR-related publications, many...

  13. Zhang Lab CRISPR Page

    Type
    Collection
    ...DA, Weinstein JA, Ran FA, Konermann S, Agarwala V, Li Y, Fine EJ, Wu X, Shalem O, Cradick TJ, Marraffini...CRISPR-Cas9. Swiech L, Heidenreich 1, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol . ... of Cas9 in vivo and ex vivo , the Zhang Lab established Cre-dependent and constitutively expressing Cas9...

  14. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...phosphoserine resource for phosphoproteomics. Gassaway BM, Li J, Rad R, Mintseris J, Mohler K, Levy T, Aguiar M... and a new pSerOTS. Recently the Rinehart lab published a new strain of recoded E.coli (rEcoli XpS 192872...

  15. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Zhao F, Luo MH, Gong L, He M, Zhou P, Paninski L, Li B. 2017. The central amygdala controls learning in...

  16. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Ac5 Parkinson's Elisa Izaurralde 147087 pLNHA-C1-HseIF4G_O EIF4G HA CMV Parkinson's Elisa Izaurralde 147091...Ac5 Parkinson's Elisa Izaurralde 148112 pcDNA3.1-MS2-HA-HshnRNPA1_Z HNRNPA1 CMV ALS Elisa Izaurralde 148113...proteinopathy, ALS Elisa Izaurralde 148138 pT7-V5-SBP-C1-HshnRNPA1_Z HNRNPA1 CMV ALS Elisa Izaurralde 148139...proteinopathy, ALS Elisa Izaurralde 148154 pnEA-NvM-DmeIF4G_578-650-GB1_Z EIF4G Parkinson's Elisa Izaurralde ...Parkinson's Elisa Izaurralde 148504 pnEA-NpM-DmeIF4G_578-675-GB1_AD EIF4G Parkinson's Elisa Izaurralde ...Parkinson's Elisa Izaurralde 148506 pnEA-NpM-DmeIF4G_601-660-GB1_AD EIF4G1 Parkinson's Elisa Izaurralde...Parkinson's Elisa Izaurralde 148508 pnEA-NpM-DmeIF4G_617-634-GB1_AD EIF4G Parkinson's Elisa Izaurralde ...

  17. COVID-19 Resources

    Type
    Collection
    ...of spike protein mutations. Research Articles : A list of free access articles on COVID-19 research. Protocols...be made available as soon as possible. For a full list of searchable plasmids, see the table below. SARS-CoV...generating SARS-CoV-2 spike pseudotyped virus. It also lists several luciferase and fluorescent reporter plasmids...see our Plasmid Distribution to Industry page. The list of plasmids available to industry is continually... cytokine storm response in the body. For a full list of cytokines and related plasmids see our Immunology...SHERLOCK and SHERLOCKv2). The Broad Institute has published information and protocols for utilizing SHERLOCK...Trans Reporter (DETECTR). Mammoth Biosciences has published information and protocols for utilizing DETECTR...

  18. Antibody Guide

    Type
    Collection
    ...two most common types of ELISAs are direct or sandwich ELISAs. For direct ELISAs, an antigen or protein ...expression and assay efficacy. ELISA Enzyme-linked immunosorbent assay (ELISA) is a plate-based assay that...differentiate between proteins. HRP is also used in ELISAs, which only measure a single antibody signal. Here...relative amounts of protein. Includes: Western blot ELISA Capture - used to isolate specific proteins or protein...Quantification methods, such as western blots or ELISAs, are used to quantify relative amounts of protein...standard curves to quantify exact amounts of proteins. ELISAs quantify a single protein per assay and typically...signaling molecule. Figure 4: A direct sandwich ELISA, which uses a capture antibody and a primary conjugated...

  19. TALEN Plasmids and Kits

    Type
    Collection
    ...List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE...pC-GoldyTALEN Golden Gate TALEN 2.0 36033 pTAL5-BB Tom Ellis Plasmids pTAL5-BB and pTAL6-BB are designed as alternative... domain of a TALE, along with strong nuclear localisation tags and repress transcription initiation when...the pTAL3 TALEN backbone, which were initially published by Sangamo BioSciences (N153AA, C63AA) and showed...improved, truncated TALE backbone architecture as established by Miller et al. (PMID: 21179091), as well as...regulation of nearby gene expression. The vectors listed below have the EF1α promoter for efficient mammalian... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help...

  20. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...Please note that the following table lists both published and unpublished plasmids containing gRNA sequences... Design Tools CRISPR Blog Posts The table below lists gRNA plasmids designed to target various genes or...deposit process and have your plasmids added to the list. You can also email [email protected] for more information... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help...
Showing: 1 - 20 of 125 results