...currently shared amongst the zebrafish community. Don E, et al. Zebrafish. 2017. PubMed PMID: 27631880 Kwan...Bacterial Expression Construct SpCas9 Inhibition (E. coli) Mammalian Expression Construct SpCas9 Inhibition...improve the study of specific neuronal cell types involved in neurodegenerative diseases, the Cole Lab selected...to premature aging and cancer. The Denchi lab investigated an aspect of telomere length regulation called...

...doi.org/10.21203/rs.3.rs-2941917/v1.  Ivorra-Molla, E., et al. (2023). A monomeric StayGold fluorescent ...adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene delivery in non-human primate (NHP...This new capsid will be a valuable tool for investigating NHP brains. Figure 3: Overview of library...

...Bourges D, Ersing I, Haery L, Harten DeMaio K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L...things at Addgene, each part of this new process involves a significant amount of QC. First, we perform ...

...Pubmed Central PMCID: PMC1222268. 7. Aronson, Deborah E., Lindsey M. Costantini, and Erik L. Snapp. "Superfolder...the cytoplasm. That is, unfortunately, some investigators realize too late that they have missed the impact...behaving properly, FPs enable researchers to investigate the localization and dynamics of fluorescent ...environment where it will localize [4]. Most investigators are surprised to learn that FPs are often poorly...overestimation of fusion protein activity. We encourage investigators to characterize FP-fusion proteins functionally...

... CBE Life Sciences Education. 21(4):ar74. Duboue, E., Kowalko, J., Keene, A. et al. 2022. Course-based...ownership of their work and become more actively involved in the learning process. As trainees become more... and effort and/or perceived professional risk involved in setting up a CURE, especially if it’s a new...

...strain.  Most Addgene plasmids are maintained in the E. coli lab strain DH5a, but others may require strains...? Depositing lab: The name of the Principal Investigator (PI) in whose lab the construct was made. The...

...standardized assembly of multigene constructs. Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. PLoS...that will provide a similar resource for those involved in monocot engineering projects. The MoClo tools...

...approach is to express and purify His-tagged Cas9 from E. coli using a plasmid such as pET-28b-Cas9-His from...Chen et al. 2016). For CRISPR experiments that involve HDR, electroporation can be combined with cell-...

...Repeat) sequences were initially discovered in the E. coli genome in 1987, but their function as a safeguard...Mammalian systems (Human, mouse, and rat) Bacteria ( E. coli , Streptococcus , Streptomyces , and others)...Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. 2012. A programmable dual-RNA-guided DNA endonuclease... Saunders SJ, Barrangou R, Brouns SJ, Charpentier E, Haft DH, Horvath P, Moineau S, Mojica FJ, Terns RM...9. PMID: 26355004 Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelson T, Heckl D, Ebert BL, ...as a powerful defensive strategy against viral invaders, and this system is proving to be just as useful...CRISPR-associated (Cas) genes to not only store a record of invading phages but also to destroy the phages upon re-...

...2003): 563-573. PubMed PMID:12490562. Karess, Roger E., et al. "The regulatory light chain of nonmuscle ...factors to repress the expression of genes primarily involved in development. These genes are known to play ... a comic book his daughter was reading. SHH is involved in the hedgehog signalling pathway, which is a...resemble spaghetti. We have two plasmids in our inventory from the lab of Eric Wieschaus that express sqh...Azplazu and Frasch, 1993). Tinman is specifically involved in the formation of the heart amongst other things...Transcriptional activation in Drosophila spermatogenesis involves the mutually dependent function of aly and a novel...

...for eukaryotes. At the time, bacterial systems from E. coli presented the best candidates for inducible ...silent in absence of binding tTA or rTA; has high copy E. coli origin None Either Ralser 64238 pTet-IRES-EGFP... Svensjö T, Winkler T, Lu M, Eriksson C, Eriksson E. Hum Gene Ther . 1998 Sep 1;9(13):1939-50. PubMed ...improved animal models for biomedical research. Bockamp E, Maringer M, Spangenberg C, Fees S, Fraser S, Eshkind...this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource...which can result in unintended system induction. Investigate whether or not your serum is tetracycline-free...

...grid.   References Bischof J, Duffraisse M, Furger E, Ajuria L, Giraud G, Vanderperre S, Paul R, Björklund... to be successful. A BiFC experiment generally involves three steps: fusion protein construction, cellular...

...PubMed Central PMCID: PMC6214761. Savell, Katherine E., et al. "A neuron-optimized CRISPR/dCas9 activation...singled out two Bdnf isoforms (I and IV) for investigation. Co-transduction of primary rat neurons with...

...Nanobodies are easily expressed in the cytosol of E. coli, however the nanobody proteins tend to get stuck... at small and large scale.  This new technique involves selection in the cytosol (instead of on the yeast...

...PubMed Central PMCID: PMC5529245. Deverman, Benjamin E., et al. "Cre-dependent selection yields AAV variants...Ken Y., et al. "Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous...

...Guides Plasmids 101: E. coli strains for protein expression Plasmids 101: Common Lab E. coli Strains Why ...Why Bacteria? E. coli are commonly used to store and replicate plasmids of all types, but beyond that, researchers... researchers also use bacteria like E. coli and their relatively well understood biology to answer many...expression of an N-terminally His-tagged TALEN fused to an E. coli codon optimized FokI endonuclease domain. pET-Sangamo-His...expression of a C-terminally His-tagged TALEN fused to an E. coli codon optimized FokI endonuclease domain. Return...that can be used to express a protein of interest in E. coli , B. subtilis and P. pastoris . All plasmids...this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource...

...Nilaratanakul, Voraphoj, Debra A. Hauer, and Diane E. Griffin. "Development and characterization of sindbis...2013). Since then, Spinach2 has been used to investigate intracellular viral DNA dynamics in neuronal ...developed into a split aptamer that can be used to investigate RNA assembly. The split aptamer is composed of... blog Learn more about aptamer fluorophores Investigate other fluorescent biosensors Find other uses ...

...16 33,170 Bikard Lab E. coli Genome-wide Inhibition Library v1 115927 Inhibition E. coli Bikard N/A 19 ... Bikard Lab EcoWG1: E. coli Genome-wide Inhibition Library v2 131625 Inhibition E. coli Bikard N/A ∼5 ...Chong Zhang E. coli Genome-wide Inhibition Library 113134 — 113138 113146, 113147 Inhibition E. coli Chong...Base Editing Species Human Mouse Fly Yeast T. gondii E. coli S. pneumoniae M. tuberculosis M. smegmatis Green...

...increase its specificity. 2018. Lee JK, Jeong E, Lee J, Jung M, Shin E, Kim YH, Lee K, Jung I, Kim D, Kim S, ...screens in E. coli identify essential genes and phage host factors. 2018. Rousset F, Cui L, Siouve E, Becavin...specificity and lower off-target activity. By screening E. coli cells transformed with a pooled library of SpCas9...transduce cells with the lentiviral library (panel E ). Remember - if you are using a 2-vector system, ...creating premature stop codons NHEJ N on- H omologous E nd J oining; A DNA repair mechanism that often introduces...Khedher AHY, Menoret S, Brion A, Lamribet K, Dardillac E, Boix C, Perrouault L, Tesson L, Geny S, De Cian A...of CRISPR-Cas9. 2017. Harrington LB, Doxzen KW, Ma E, Liu JJ, Knott GJ, Edraki A, Garcia B, Amrani N, Chen...

...Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez H, Hiltke T, Snyder M, Yamamoto...sample type. Sample Preparation This step can involve multiple smaller steps that all help to ensure ... Similar to Sample Preparation, this step can involve multiple smaller steps, but the aim is to preserve...