...production of viral particles, and transduction of a target cell line with those particles. Since the Env,...Nervous about handling viruses? That’s okay! In this article we will discuss general viral safety considerations...you’ll need to take extra steps to ensure that particles are not sprayed into the air. For lentivirus and...

... will highlight four tools for finding journal articles related to your actual interests while also staying...publication date, a relevance score, and type of article. Now, search results display a bar graph of the...PubMed entry are great places to look for more articles on your topic of interest.  Best uses Accessing... including a bot for biosensor papers, microbe articles, CRISPR papers, and more. Saving papers There...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid... polyomavirus virus-like particles (MPyV VLPs ). This artificial nanoparticle can protect proteins from...

...lentivirus, AAV requires a higher number of viral particles for efficient gene transfer. I figure it’s better...alone. If yield is important, or if the viral particles of the AAV serotype you’re working with are primarily...ethanol and dry ice bath to release the virus particles from the cells. Cell lysates are then treated ...quantifies the number of genome-containing viral particles that are present in an AAV prep. AAV samples are...

...suppressors to the accelerator and brake pedal of a vehicle, respectively. In the case of a mutation that turns...oncogene), this is analogous to the accelerator of a vehicle being stuck down. Similarly, a mutation that turns...the same result – uncontrolled movement of the vehicle or, in the case of cancer, uncontrolled cell proliferation...non-cancerous cells, such as those within hair follicles and lining the digestive system, causing discomfort...

...distributed as 100 µL solutions of purified viral particles that are suitable for in vivo use. Minimum titers...

...mCardinal 604/659 18 All mCardinal plasmids from article mNeptune 600/650 13.4 pcDNA3-mNeptune-N E2-Crimson...

...new window) Pubmed (Link opens in a new window) Article Individual plasmids can be ordered via the links...

...acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene...

...new window) PubMed (Link opens in a new window) Article . Individual plasmids can be ordered via the links...

...USA SynBio initiative Recent Synthetic Biology Articles - Peccoud lab social media tracker Synthetic Biology...

... express plasmid-based antibodies. Description Article PI One-step assembly of antibody heavy- and light-chain...

...ribonucleoprotein delivery by lentiviral capsid-based bionanoparticle Transient expression of CRISPR/Cas9 in cells...’s lab developed a lentiviral capsid-based bionanoparticle system for packaging Cas9/gRNA ribonucleoproteins...binding protein for packaging into a lentivirus-like particle. Read the paper in Nucleic Acids Research Find...

...Institute for Cell Science has produced the first publicly available collection (Link opens in a new window...

...Additionally, a concurrent Nature Biotechnology article describes the development of a mouse lentiviral...genomic screens Using lentivirus as the delivery vehicle, Neville Sanjana, also of the Broad Institute, ...M, Yusa K. Nature Biotechnology. 2013 Dec 23. (Article). Read more about CRISPRs: Using CRISPR/Cas to...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...plasmids you need. Here’s what you’ll find in this article: CRISPR-based editing in organoid Biosensors for...

...of our Hot Plasmid articles on our website. We update the our Hot Plasmids articles every quarter so be... expression. The plasmids associated with this article can be obtained through Addgene: Rapamycin-inducible...course of gene activation using this system in the article cited below. Scientists can obtain dCas9-trCIB1...trCIB1, CRY2PHR-p65, and other plasmids from this article through Addgene. Nihongaki et al., Chemistry and...TOP2 constructs; see Supplementary Note 3 in the article for more info) are available. Furthermore, there...

...days. In an open access Nucleic Acids Research article that came out in the spring of 2011, the groups...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...plasmids you need. Here's what you'll find in this article: An improved cell-free system to examine biosynthetic...

...the battery bank and the vehicle battery/alternator to make sure the vehicle battery didn't run down. ...100°C. They published their finding in a bioRxiv article and in PNAS. Two other thermostable Cas9 enzymes...