...limitations (Figure 1). Figure 1: Opto-katanin induces fast, localized microtubule severing. A) Schematic of... below use the following optogenetic modules: VVDfast homodimerizes under blue light iLID-SspB heterodimerizes...of Opto-katanin constructs and activity. EB3N-VVDfast-iLID (anchor) and SspB-mCherry-p60 (katanin) are... of two separate blue light-sensitive systems, VVDfast to control recruitment of an “anchor” to microtubules...anchor. So, you’ll need to express both the EB3N-VVDfast-iLID anchor module and the SspB-mCherry-p60 katanin...controlling cell dynamics. Need a refresher on VVDfast, iLID, LOV, CRY2-CIB, and other optogenetic modules...

...contribute to the popularity of GFP including (i) fast and complete maturation to functional, fluorescent...

...varies for different enzymes. Many companies now sell fast digest enzymes that can digest large amounts of ...

... find today are marketed for a specific purpose: fast growth, high-throughput cloning, routine cloning...

... image all the different colors in one session – fast and easy! Just make sure your primary antibodies...

...appropriate cell growth conditions. Note: if using fast-growing bacteria like E. coli or Salmonella, or ...

... how chaotic and random the scanning was and how fast T cells could jump from one antigen presenting cell...

... later became known as SNAP-tag. There is also a fast-reacting version of SNAP-tag called SNAPf. eDHFR...ligands (Wilhelm et al., 2021). While HaloTag reacts faster for some dyes, others, especially some nonfluorescent...

...Hideaki Mizuno, and Atsushi Miyawaki. "Regulated fast nucleocytoplasmic shuttling observed by reversible...

...gene that is shared across all CRISPR types; the fast evolution of these adaptive bacterial and archaeal...

...dependency is a severe limitation, but the tool is fast enough that, if you’re engaged in heavy CRISPR design...formatted output. Surprisingly only 2 tools provide FASTA output. A few notable tools allow the user to save...

...less time to complete. Morphology is a simple and fast to evaluate. iPSCs should form compact colonies ...reprogramming period and the plasmid will get diluted faster when cells are actively dividing. The typical drawbacks...

...actuator pair. Ideally, indicators should possess fast voltage sensing kinetics and have no spectral overlap...named CheRiff, exhibits high light sensitivity and fast kinetics. By combining both the voltage indicator...visualize the Broccoli-tagged RNA. This technique is faster and less labor intensive than northern blotting...

...optimizing protein expression and purification in a fast, efficient and affordable way. Scholz et al.., BMC...Chronos, is blue and green light-excitable and has faster kinetics than those of other channelrhodopsins....

...transgenesis Insect / Baculovirus Polyhedrin pFastBac LIC and pFastBac Dual LIC - Vectors from...

...files) or single-end reads (single file) in FASTQ format (fastq.gz files are also accepted) from a deep sequencing...

... FUS_LC_withR FUS T7 ALS Nicolas Fawzi 139211 pFastBac1-LRRK2 WD40 LRRK2 His, MBP polH Parkinson's Hao...S473D OPTN GST-TEV ALS Sascha Martens 187830 pFastBac_Dual_GST-TEV-EGFP-TBK1 TBK1 GST, TEV-mEGFP-GG Linker...6His HTRA2 His T7 Parkinson's Eric Fischer 198033 pFastBac-GST-TEV-mCherry-TBK1 TBK1 mCherry, GST, Tev P10...P10 ALS Sascha Martens 198037 pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K TBK1 GFP, GST, Tev P10 ALS Sascha ...Sascha Martens 198072 pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666) TBK1 GFP, GST, Tev P10 ALS Sascha Martens...) OPTN GFP, FKBP CMV ALS Sascha Martens 208875 pFastBac-Dual- GST-TEV-TBK1 TBK1 polH ALS Sascha Martens...

..., we can save you time and help you get results faster. Choose from one of the options below to get started...

...Speakers and Attendees Friday, September 21 9:00 AM Breakfast and Welcome Melina Fan 9:30 AM Cre and Flpo Katherine...

...TGCCCTCCAATGGCAGCAaagcccttaagcttcaATTCGGGCTTATTAATCA fastkd3 TAL3438 & TAL3439 TACCTGTCTTCTAATGAGgaccacgcttttatgaAACGCCTCTCCTCTTGTA...